• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidating the basis of plant cells based on the organelle division mechanism of microalgae

Research Project

Project/Area Number 19H03260
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44040:Morphology and anatomical structure-related
Research InstitutionJapan Women's University

Principal Investigator

KUROIWA Tsuneyoshi  日本女子大学, 理学部, 研究員 (50033353)

Co-Investigator(Kenkyū-buntansha) 永田 典子  日本女子大学, 理学部, 教授 (40311352)
八木沢 芙美  琉球大学, 研究基盤センター, 准教授 (70757658)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2021: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywordsオルガネラ分裂同調化 / オルガネラ分裂装置 / 細胞質分裂 / 細胞核分裂 / ミトコンドリア分裂 / 葉緑体分裂 / ペルオキシソーム分裂 / TOP / オルガネラ分裂高度同調化 / オルガネラ分裂・細胞質分裂 / ESCRT-III / VPS4 / オルガネラ / ゲノム形態学 / 分裂マシン / 超微細藻類 / シゾン / メダカモ / 高度同調培養系 / オルガネラ分裂 / DYNAMO-II / ミトコンドリア分裂装置 / DYNAMO1 / オーロラキナーゼ / ESCRT / 超微細構造 / ダイナミン
Outline of Research at the Start

本研究の概要は、細胞質分裂に先立つオルガネラの分裂の共通機構を解明し、それを基盤に細胞分裂の全貌を明らかにすることである。ミトコンドリアと色素体(葉緑体)が核(核様体;DNAとタンパク質複合体)を持ち、核分裂後、分裂リング(PDリング, MDリング)とダイナミン等の複合体(分裂マシン)を使って分裂/増殖していることを発見した。最近単膜系のペルオキシソームでも分裂マシン(PODリング)を見出した。こうしたオルガネラの分裂/増殖の機構を基盤に、細胞質分裂に至る分裂系の全体像を解明することである。

Outline of Final Research Achievements

Dynamics of organelle division / proliferation, etc. in eukaryotic cell proliferation, and the mechanism that leads the information to the cell nucleus and cytokinesis was studied using the primitive red alga C. merolae as a material. Each organelle divides sequentially using a division device, and each division. The constituents of the device were confirmed. It was revealed that TOP (kinesin-like substance) acts on the distribution of divided organelles and then is involved in cell fission. Subsequently, the cell membrane contractile substance (EF1α) functioned, and then molecules involved in central cell membrane disruption (ESCRT-III, ALIX, VPS4, etc.) appeared and divided. ESCR-III was presumed to form a complex with an "electron-dense ring" and cause final fragmentation, and similar rings were also observed in Medakamo. In addition, a metaphase chromosomal structure was confirmed in Schyzon.

Academic Significance and Societal Importance of the Research Achievements

多様な真核生物を形成する真核細胞の基本構造と機能を解明することにより、細胞増殖のしくみが求められる医学(病気や癌の治療等)、農学(食糧の増産等)に利用される。既に報告したように、本研究の研究協力者の井元祐太博士によってシゾンで見出されたDYNAMO1が、ジョンズホプキンス大学から、癌の転移の阻止に有効との研究が公表されている。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (24 results)

All 2021 2020 2019 Other

All Int'l Joint Research (2 results) Journal Article (11 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 11 results,  Open Access: 10 results) Presentation (11 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Int'l Joint Research] ジョンズホプキンス大学医学部(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] ジョンズ・ホプキンス大学医学部(米国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Smooth Loop-Like Mitochondrial Nucleus in the Primitive Red Alga <i>Cyanidioschyzon merolae</i> Revealed by Drying Treatment2021

    • Author(s)
      Kuroiwa, T., Yagisawa, F., Fujiwara, T., Misumi, O., Nagata, N., Imoto, Y., Yoshida, Y., Mogi, Y., Miyagishima, SY. and Kuroiwa, H.
    • Journal Title

      CYTOLOGIA

      Volume: 86 Issue: 1 Pages: 89-96

    • DOI

      10.1508/cytologia.86.89

    • NAID

      130008002729

    • ISSN
      0011-4545, 1348-7019
    • Year and Date
      2021-03-25
    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Existence of giant mitochondria-containing sheet structures lacking cristae and matrix in etiolated cotyledon of Arabidopsis thaliana2021

    • Author(s)
      Fukushima, S., Akita, K., Takagi, T., Kobayashi, K., Moritoki, N., Arimura, S., Kuroiwa, H., Kuroiwa, T. and Nagata, N.
    • Journal Title

      Protoplasma

      Volume: 259 Issue: 3 Pages: 731-742

    • DOI

      10.1007/s00709-021-01696-0

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ultrastructural characterization of microlipophagy induced by the interaction of vacuoles and lipid bodies around generative and sperm cells in Arabidopsis pollen.2021

    • Author(s)
      K. Akita, T. Takagi, K. Kobayashi, K. Kuchitsu, T. Kuroiwa, N. Nagata
    • Journal Title

      Protoplasma

      Volume: 258 Issue: 1 Pages: 129-138

    • DOI

      10.1007/s00709-020-01557-2

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recent insights into peroxisome biogenesis and associated diseases2020

    • Author(s)
      *Fujiki, Y., Abe, Y., Imoto, Y., Tanaka, A.J., Okumoto, K., Honsho, M., Tamura, S., Miyata, N., Yamashita, T., Chung, W.K., and Kuroiwa, T.
    • Journal Title

      J. Cell Sci.

      Volume: 133 Issue: 9 Pages: 236943-236943

    • DOI

      10.1242/jcs.236943

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mitotic karyotype of the primitive red alga Cyanidioschyzon merolae 10D2020

    • Author(s)
      Kuroiwa, T., Yagisawa, F., Fujiwara, t., Inui, Y., Matsunaga, S., Kato, S., Matsunaga, S., Nagata, N., Imoto, Y. and Kuroiwa, H.
    • Journal Title

      Cytologia

      Volume: 85 Pages: 107-113

    • NAID

      130007864023

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Efficient open cultivation of Efficient open cultivation of Cyanidialean red algae in acidified seawater2020

    • Author(s)
      Hirooka, S., Tomita, R., Fujiwara, T., Ohnuma, M., Kuroiwa, H., Kuroiwa. T. and Miyagishima, S.
    • Journal Title

      Sci. Rep. nature research

      Volume: 10 13794 Pages: 1-14

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular basis of local energy generation during mitochondrial and peroxisomal division2020

    • Author(s)
      Imoto, Y., Abe, Y., Honsho, M., Okumoto, K., Ohnuma, M., Kuroiwa, H., Kuroiwa, T. and Fujiki, Y.
    • Journal Title

      Plant Morph

      Volume: 32 Pages: 59-73

    • NAID

      130008006485

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ESCRT machinery mediates cytokinetic abscission in the unicellular red alga Cyanidioschyzon merolae.2020

    • Author(s)
      Yagisawa F., Fujiwara T., Takemura T., Kobayashi Y., Sumiya N., Miyagishima S. Y., Nakamura S., Imoto Y., Misumi O., Tanaka K., Kuroiwa H., Kuroiwa T.
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 8 Pages: 169-169

    • DOI

      10.3389/fcell.2020.00169

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evolutionary significance of the ring-like plastid nucleus in the primitive red alga Cyanidioschyzon merolae as revealed by drying.2020

    • Author(s)
      Kuroiwa, T., Ohnuma, M., Imoto, Y., Yagisawa, Y., Misumi, O., Nagata, N. and Kuroiwa, H.
    • Journal Title

      Protoplasma

      Volume: 257 Pages: 1-12

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dynamics of the nucleoside diphosphate kinase protein DYNAMO2 correlates with the changes in the global GTP level during the cell cycle of <i>Cyanidioschyzon merolae</i>2019

    • Author(s)
      Imoto, Y., Abe, Y., Okumoto, K., Ohnuma, M., Kuroiwa, H., Kuroiwa, T., and Fujiki, Y.
    • Journal Title

      Proceedings of the Japan Academy, Series B

      Volume: 95 Issue: 2 Pages: 75-85

    • DOI

      10.2183/pjab.95.007

    • NAID

      130007591938

    • ISSN
      0386-2208, 1349-2896
    • Year and Date
      2019-02-08
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cyanidioschyzon merolae aurora kinase phosphorylates evolutionarily conserved sites on its target to regulate mitochondrial division2019

    • Author(s)
      Kato Shoichi、Okamura Erika、Matsunaga Tomoko M.、Nakayama Minami、Kawanishi Yuki、Ichinose Takako、Iwane Atsuko H.、Sakamoto Takuya、Imoto Yuuta、Ohnuma Mio、Nomura Yuko、Nakagami Hirofumi、Kuroiwa Haruko、Kuroiwa Tsuneyoshi、Matsunaga Sachihiro
    • Journal Title

      Communications Biology

      Volume: 2 Issue: 1 Pages: 477-477

    • DOI

      10.1038/s42003-019-0714-x

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 真核細胞の細胞小器官の分裂情報を細胞質分裂に伝達する機構のゲノム形態学による解明2021

    • Author(s)
      黒岩常祥 黒岩晴子 永田典子 八木沢芙美 三角修己 藤原崇之 乾弥生 松永朋子 加藤翔一 井元祐太 吉田大和 田草川真理 松永幸大
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] フウロソウ科植物の胚発生過程における細胞内オルガネラの変動について―22021

    • Author(s)
      黒岩晴子 永田典子 黒岩常祥
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 単細胞紅藻イデユコゴメ類の海水を用いた培養技術開発2021

    • Author(s)
      廣岡俊亮 富田麗子 藤原嵩之 大沼みお 黒岩晴子 黒岩常祥 宮城島進也
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] シゾンの中期染色体の発見から見えてきたメダカモでの真核生物増殖の原理の解明2020

    • Author(s)
      黒岩常祥、八木沢芙美、 永田典子、三角修己、藤原崇之、乾弥生、松永朋子、加藤翔一、井元祐太、黒岩晴子、田草川真理、松永幸大
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 高等植物の卵細胞内オルガネラの胚発生過程における変動2020

    • Author(s)
      黒岩晴子、永田典子、黒岩常祥
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロイヌナズナ花粉のリポファジーに関する電子顕微鏡観察2020

    • Author(s)
      秋田佳恵、高木智子、小林啓子、朽津和幸、黒岩常祥、永田典子
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 真核植物の誕生と進化をシゾンとメダカモのオルガネラ核と分裂装置から解く2019

    • Author(s)
      黒岩常祥 黒岩晴子 永田典子 三角修己 田草川真理 松永幸大 加藤翔一
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高等植物の重複受精前後における胚嚢内細胞の動態について―22019

    • Author(s)
      黒岩晴子 永田典子 黒岩常祥
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 原始紅藻Cyanidioschyzon merolaeにおけるポリリン酸キナーゼPPK1の解析2019

    • Author(s)
      八木沢芙美 藤原貴之 宮城島進也 三角修己 中村宗一 黒岩晴子 黒岩常祥
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 愛媛県鈍川温泉由来の油脂生産藻類とその脂肪滴2019

    • Author(s)
      大沼みお 黒岩晴子 黒岩常祥 広岡俊亮 藤原貴之 宮城島進也 眞田宣明
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 私たちの真核細胞はどのように生まれたか?-オルガネラの分裂装置と遺伝に基づく解析2019

    • Author(s)
      黒岩常祥
    • Organizer
      第14回 日韓学術フォーラム(韓国ソウル大学)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited

URL: 

Published: 2019-04-18   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi