Elucidating the basis of plant cells based on the organelle division mechanism of microalgae
Project/Area Number |
19H03260
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 44040:Morphology and anatomical structure-related
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Research Institution | Japan Women's University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
永田 典子 日本女子大学, 理学部, 教授 (40311352)
八木沢 芙美 琉球大学, 研究基盤センター, 准教授 (70757658)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Project Status |
Completed (Fiscal Year 2021)
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Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2021: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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Keywords | オルガネラ分裂同調化 / オルガネラ分裂装置 / 細胞質分裂 / 細胞核分裂 / ミトコンドリア分裂 / 葉緑体分裂 / ペルオキシソーム分裂 / TOP / オルガネラ分裂高度同調化 / オルガネラ分裂・細胞質分裂 / ESCRT-III / VPS4 / オルガネラ / ゲノム形態学 / 分裂マシン / 超微細藻類 / シゾン / メダカモ / 高度同調培養系 / オルガネラ分裂 / DYNAMO-II / ミトコンドリア分裂装置 / DYNAMO1 / オーロラキナーゼ / ESCRT / 超微細構造 / ダイナミン |
Outline of Research at the Start |
本研究の概要は、細胞質分裂に先立つオルガネラの分裂の共通機構を解明し、それを基盤に細胞分裂の全貌を明らかにすることである。ミトコンドリアと色素体(葉緑体)が核(核様体;DNAとタンパク質複合体)を持ち、核分裂後、分裂リング(PDリング, MDリング)とダイナミン等の複合体(分裂マシン)を使って分裂/増殖していることを発見した。最近単膜系のペルオキシソームでも分裂マシン(PODリング)を見出した。こうしたオルガネラの分裂/増殖の機構を基盤に、細胞質分裂に至る分裂系の全体像を解明することである。
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Outline of Final Research Achievements |
Dynamics of organelle division / proliferation, etc. in eukaryotic cell proliferation, and the mechanism that leads the information to the cell nucleus and cytokinesis was studied using the primitive red alga C. merolae as a material. Each organelle divides sequentially using a division device, and each division. The constituents of the device were confirmed. It was revealed that TOP (kinesin-like substance) acts on the distribution of divided organelles and then is involved in cell fission. Subsequently, the cell membrane contractile substance (EF1α) functioned, and then molecules involved in central cell membrane disruption (ESCRT-III, ALIX, VPS4, etc.) appeared and divided. ESCR-III was presumed to form a complex with an "electron-dense ring" and cause final fragmentation, and similar rings were also observed in Medakamo. In addition, a metaphase chromosomal structure was confirmed in Schyzon.
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Academic Significance and Societal Importance of the Research Achievements |
多様な真核生物を形成する真核細胞の基本構造と機能を解明することにより、細胞増殖のしくみが求められる医学(病気や癌の治療等)、農学(食糧の増産等)に利用される。既に報告したように、本研究の研究協力者の井元祐太博士によってシゾンで見出されたDYNAMO1が、ジョンズホプキンス大学から、癌の転移の阻止に有効との研究が公表されている。
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Smooth Loop-Like Mitochondrial Nucleus in the Primitive Red Alga <i>Cyanidioschyzon merolae</i> Revealed by Drying Treatment2021
Author(s)
Kuroiwa, T., Yagisawa, F., Fujiwara, T., Misumi, O., Nagata, N., Imoto, Y., Yoshida, Y., Mogi, Y., Miyagishima, SY. and Kuroiwa, H.
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Journal Title
CYTOLOGIA
Volume: 86
Issue: 1
Pages: 89-96
DOI
NAID
ISSN
0011-4545, 1348-7019
Year and Date
2021-03-25
Related Report
Peer Reviewed
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[Journal Article] Existence of giant mitochondria-containing sheet structures lacking cristae and matrix in etiolated cotyledon of Arabidopsis thaliana2021
Author(s)
Fukushima, S., Akita, K., Takagi, T., Kobayashi, K., Moritoki, N., Arimura, S., Kuroiwa, H., Kuroiwa, T. and Nagata, N.
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Journal Title
Protoplasma
Volume: 259
Issue: 3
Pages: 731-742
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Recent insights into peroxisome biogenesis and associated diseases2020
Author(s)
*Fujiki, Y., Abe, Y., Imoto, Y., Tanaka, A.J., Okumoto, K., Honsho, M., Tamura, S., Miyata, N., Yamashita, T., Chung, W.K., and Kuroiwa, T.
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Journal Title
J. Cell Sci.
Volume: 133
Issue: 9
Pages: 236943-236943
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Mitotic karyotype of the primitive red alga Cyanidioschyzon merolae 10D2020
Author(s)
Kuroiwa, T., Yagisawa, F., Fujiwara, t., Inui, Y., Matsunaga, S., Kato, S., Matsunaga, S., Nagata, N., Imoto, Y. and Kuroiwa, H.
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Journal Title
Cytologia
Volume: 85
Pages: 107-113
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Efficient open cultivation of Efficient open cultivation of Cyanidialean red algae in acidified seawater2020
Author(s)
Hirooka, S., Tomita, R., Fujiwara, T., Ohnuma, M., Kuroiwa, H., Kuroiwa. T. and Miyagishima, S.
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Journal Title
Sci. Rep. nature research
Volume: 10 13794
Pages: 1-14
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Molecular basis of local energy generation during mitochondrial and peroxisomal division2020
Author(s)
Imoto, Y., Abe, Y., Honsho, M., Okumoto, K., Ohnuma, M., Kuroiwa, H., Kuroiwa, T. and Fujiki, Y.
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Journal Title
Plant Morph
Volume: 32
Pages: 59-73
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] ESCRT machinery mediates cytokinetic abscission in the unicellular red alga Cyanidioschyzon merolae.2020
Author(s)
Yagisawa F., Fujiwara T., Takemura T., Kobayashi Y., Sumiya N., Miyagishima S. Y., Nakamura S., Imoto Y., Misumi O., Tanaka K., Kuroiwa H., Kuroiwa T.
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Journal Title
Frontiers in Cell and Developmental Biology
Volume: 8
Pages: 169-169
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Evolutionary significance of the ring-like plastid nucleus in the primitive red alga Cyanidioschyzon merolae as revealed by drying.2020
Author(s)
Kuroiwa, T., Ohnuma, M., Imoto, Y., Yagisawa, Y., Misumi, O., Nagata, N. and Kuroiwa, H.
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Journal Title
Protoplasma
Volume: 257
Pages: 1-12
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Dynamics of the nucleoside diphosphate kinase protein DYNAMO2 correlates with the changes in the global GTP level during the cell cycle of <i>Cyanidioschyzon merolae</i>2019
Author(s)
Imoto, Y., Abe, Y., Okumoto, K., Ohnuma, M., Kuroiwa, H., Kuroiwa, T., and Fujiki, Y.
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Journal Title
Proceedings of the Japan Academy, Series B
Volume: 95
Issue: 2
Pages: 75-85
DOI
NAID
ISSN
0386-2208, 1349-2896
Year and Date
2019-02-08
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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