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Relationship between Soil Microbial Diversity and Priming Effects in Forest Ecosystems

Research Project

Project/Area Number 19K20434
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 63010:Environmental dynamic analysis-related
Research InstitutionNagoya University (2022)
Kyoto University (2020-2021)
Tokyo University of Agriculture and Technology (2019)

Principal Investigator

Sawada Kozue  名古屋大学, 生命農学研究科, 特任助教 (60795285)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords土壌微生物 / 土壌有機物 / プライミング効果 / 森林生態系 / 微生物群集組成 / 多様性 / アンプリコンシークエンス / 同位体プロービング法
Outline of Research at the Start

大気中炭素量の二倍以上を保持する土壌炭素量が将来どのように変動するかを予測することは、今後の気候・大気環境変動を予測する上で極めて重要である。本研究では、近年急速に発展してきた同位体分析と分子生物学的手法を応用し、日本森林生態系において、森林型(天然林と人工林)や土壌型(褐色森林土と黒ぼく土)が、炭素・窒素添加による有機物分解促進効果(プライミング効果)と土壌微生物の群集組成や多様性に与える影響を明らかにする。以上により、土壌炭素量を増加させるための森林管理法を考案する。

Outline of Final Research Achievements

This study elucidated the effects of differences in mycorrhizal types (ectomycorrhizal and arbuscular mycorrhizal fungi), soil acidity, and N and P limitation on microbial community composition and diversity and the effect of C addition to promote organic matter degradation (priming effect) in forest soils.
The results revealed that differences in mycorrhizal types significantly affected microbial community composition and diversity, but not the priming effect. The priming effect was suppressed in highly acidic soils in Japan. The priming effect through P mining occurred in P-poor tropical forest soils, and the priming effect through N mining occurred in N-poor temperate forest soils. These results are essential for accurately predicting C dynamics in forest soils.

Academic Significance and Societal Importance of the Research Achievements

プライミング効果は、菌根菌タイプの違いや微生物群集組成・多様性の違いよりも、土壌酸性度の違いや窒素・リン制限の違いの影響の方が大きいことが分かった。つまり、樹種選択による微生物群集組成の改変は、プライミング効果にはあまり影響しないかもしれない。一方、土壌酸性化は有機物分解を抑制しうること、適切な養分バランスが森林土壌の炭素蓄積に重要であることが示された。以上の成果は、森林土壌における炭素動態の予測精度の精緻化と、CO2放出量の抑制や土壌炭素量の増加に向けての適切な管理と利用法の考案につながる重要な成果である。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (11 results)

All 2023 2022 2021 2019

All Journal Article (5 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (6 results) (of which Invited: 1 results)

  • [Journal Article] Positive priming effects through microbial P-mining in tropical forest soils under N<sub>2</sub>-fixing trees2023

    • Author(s)
      Sawada Kozue、Kunito Takashi、Watanabe Tetsuhiro、Kitagawa Natsuko、Lyu Han、Nguyen Ho Lam、Toyota Koki、Funakawa Shinya
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 65 Issue: 4 Pages: 1-6

    • DOI

      10.1080/00380768.2023.2199777

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Impacts of conversion from natural forest to cedar plantation on the structure and diversity of root-associated and soil microbial communities2021

    • Author(s)
      Sawada Kozue、Inagaki Yoshiyuki、Sugihara Soh、Funakawa Shinya、Ritz Karl、Toyota Koki
    • Journal Title

      Applied Soil Ecology

      Volume: 167 Pages: 104027-104027

    • DOI

      10.1016/j.apsoil.2021.104027

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Comparison of the Structure and Diversity of Root-Associated and Soil Microbial Communities Between Acacia Plantations and Native Tropical Mountain Forests2021

    • Author(s)
      Sawada Kozue、Watanabe Shinichi、Nguyen Ho Lam、Sugihara Soh、Seki Mayuko、Kobayashi Hana、Toyota Koki、Funakawa Shinya
    • Journal Title

      Frontiers in Microbiology

      Volume: 12 Pages: 735121-735121

    • DOI

      10.3389/fmicb.2021.735121

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Soil carbon and nutrient cycles in forest and traditional agriculture ecosystems —to establish minimum loss agriculture—2021

    • Author(s)
      沢田 こずえ, 渡邉 哲弘, 舟川 晋也
    • Journal Title

      Soil Microorganisms

      Volume: 75 Issue: 2 Pages: 52-59

    • DOI

      10.18946/jssm.75.2_52

    • NAID

      130008109017

    • ISSN
      0912-2184, 2189-6518
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Priming effects induced by C and N additions in relation to microbial biomass turnover in Japanese forest soils2021

    • Author(s)
      Sawada Kozue、Inagaki Yoshiyuki、Toyota Koki
    • Journal Title

      Applied Soil Ecology

      Volume: 162 Pages: 103884-103884

    • DOI

      10.1016/j.apsoil.2021.103884

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] 菌根菌タイプが動かす森の物質循環2023

    • Author(s)
      龍見史恵、沢田こずえ
    • Organizer
      日本生態学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 熱帯林土壌におけるリンマイニングによる正のプライミング効果2022

    • Author(s)
      沢田こずえ・國頭恭・渡邉哲弘・北川夏子・Lyu Han・Nguyen Ho Lam・ 豊田剛己・舟川晋也
    • Organizer
      日本土壌肥料学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 針葉樹天然林のスギ人工林化が微生物群集組成と窒素循環に及ぼす影響2022

    • Author(s)
      沢田こずえ・稲垣善之・杉原創・國頭恭・村瀬潤・豊田剛己・舟川晋也
    • Organizer
      日本土壌肥料学会中部支部会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 菌根タイプの異なる日本森林土壌における無機態窒素と微生物群集組成の比較2021

    • Author(s)
      沢田こずえ・稲垣善之・國頭恭・杉原創・豊田剛己・舟川晋也
    • Organizer
      日本土壌肥料学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 「ミニマム・ロス」の農業の規範となる自然生態系土壌における微生物群集組成と養分循環の解明2021

    • Author(s)
      沢田こずえ
    • Organizer
      日本土壌微生物学会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 四万十川流域における天然林のスギ人工林化が土壌微生物群集組成とプライミング効果に及ぼす影響2019

    • Author(s)
      沢田こずえ・稲垣善之・渡邉哲弘・國頭恭・豊田剛己
    • Organizer
      土壌肥料学会関東支部会
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2024-01-30  

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