Structural characterization of supramolecular nucleic acid structure based on IR vibrational spectroscopy and quantum chemical calculation

• Project/Area Number: 20350012
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical chemistry
• Research Institution: Yokohama City University
• Principal Investigator: SAIGUSA Hiroyuki Yokohama City University, 大学院・生命ナノシステム科学研究科, 教授 (90162180)
• Co-Investigator(Kenkyū-buntansha): TACHIKAWA MASANORI 横浜市立大学, 生命ナノシステム科学研究科, 教授 (00267410) ; OHBA MASASHI 横浜薬科大学, 薬学部, 教授 (60115219)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
• Keywords: クラスター / 核酸塩基 / 赤外振動スペクトル / 塩基ヌクレオチド / レーザー脱離 / 非調和振動計算 / 修飾塩基 / 量子化学計算 / 化学修飾

Research Abstract

We have developed laser desorption technique for generating hydrated clusters of the DNA base pairs and those of nucleoside molecules consisting of base and sugar group, and performed structural characterization based on UV and IR spectroscopy combined with quantum mechanical calculation. We have also implemented this method for vaporizing a nucleotide having sugar-phosphate backbone, and identified the formation of a supramolecular structure.

Research Products

• [Journal Article] Mass Spectrometry for Nonvolatile Functional Molecules by Laser Desorption/Vacuum-Ultraviolet Photoionization Method (2011)
  • Author(s): R. Kikkawa, Y. Inokuchi, T. Ebata, H.Saigusa
  • Journal Title: BECKNIK(ロシアオレンブルグ大学紀要)
• [Journal Article] レーザー脱離法による核酸塩基ヌクレオチドの気相孤立化-リン酸基のエステル化による非破壊性向上 (2011)
  • Author(s): 三枝洋之
  • Journal Title: 横浜市立大学叢書 61巻 Pages: 91-100
  • NAID: 120005670481
• [Journal Article] Mass Spectrometry for Nonvolatile Functional Molecules by Laser Desorption/Vacuum-Ultraviolet Photoionization Method (2011)
  • Author(s): R.Kikkawa, Y.Inokuchi, T.Ebata, H.Saigusa
  • Journal Title: BECKNIK (ロシアオレンブルグ大学紀要) Volume: 4 Pages: 111-113
  • Peer Reviewed
• [Journal Article] Mass Spectrometry for Nonvolatile Functional Molecules by Laser Desorption/Nacuum-Ultraviolet Photoionization Method (2011)
  • Author(s): 三枝洋之
  • Journal Title: 横浜市立大学叢書 Volume: 61 Pages: 91-100
• [Journal Article] Gas-phase Isolation of Diethylguanosine 5'-monophosphate and Its Conformational Assignment (2010)
  • Author(s): H. Asami, M. Tsukamoto, Y. Hayakawa, H.Saigusa
  • Journal Title: Phys.Chem.Chem.Phys.(communication) 12 Pages: 13918-13921
  • Peer Reviewed
• [Journal Article] Microhydration of the Guanine-guanine and Guanine-cytosine Base Pairs (2010)
  • Author(s): S. Urashima, H. Asami, M. Ohba, H.Saigusa
  • Journal Title: J.Phys.Chem A (invited article) 114(Most Read Articles) Pages: 11231-11237
  • Peer Reviewed
• [Journal Article] Microhydration of the guanine-guanine and guanine-cytosine base pairs (2010)
  • Author(s): S.Urashima, H.Asami, M.Ohba, H.Saigusa
  • Journal Title: Journal of Physical Chemistry A Volume: 114 Pages: 11231-11237
  • Peer Reviewed
• [Journal Article] Gas-phase isolation of diethyl guanosine 5'-monophosphate and its conformational assignment (2010)
  • Author(s): H.Asami, M.Tsukamoto, Y.Hayakawa, H.Saigusa
  • Journal Title: Physical Chemistry Chemical Physics Volume: 12 Pages: 13918-13921
  • Peer Reviewed
• [Journal Article] Microhydration of the guanine-guanine and guanine-cytosine base pairs (2010)
  • Author(s): S.Urashima, H.Asami, М.Ohba, H.Saigusa
  • Journal Title: Journal of Physical Chemistry A (accepted for publication)
  • Peer Reviewed
• [Journal Article] Hydration Structures of 2'-Deoxyguanosine Studied by IR-UV Double Resonance Spectroscopy : Comparison with Guanosine (2009)
  • Author(s): H. Asami, S. Urashima, H.Saigusa
  • Journal Title: Phys.Chem.Chem.Phys. 11 Pages: 10466-10472
  • Peer Reviewed
• [Journal Article] IR-UV Double Resonance Spectroscopy of the Hydrated Clusters of Guanosine and 9-Methylguanine : Evidence for Hydration Structures Involving the Sugar Group (2009)
  • Author(s): H.Saigusa, S. Urashima, H. Asami
  • Journal Title: J.Phys.Chem.A. 113 Pages: 3455-3462
  • Peer Reviewed
• [Journal Article] Hydration structures of 2'-deoxyguanosine studied by IR-UV double resonance spectroscopy : Comparison with guanosine (2009)
  • Author(s): H.Asami, S.Urashima, H.Saigusa
  • Journal Title: Physical Chemistry Chemical Physics 11 Pages: 10466-10472
  • Peer Reviewed
• [Journal Article] IR-UV double resonance spectroscopy of the hydrated clusters of guanosine and 9-methylguanine : Evidence for hydration structures involving the sugar group (2009)
  • Author(s): H.Saigusa, S.Urashima, H.Asami
  • Journal Title: Journal of Physical Chemistry A 113 Pages: 3455-3462
  • Peer Reviewed
• [Journal Article] IR-UV double resonance spectroscopy of the hydrated clusters of guanosine and 9-methylguanine : Evidence for hydration structures involving the sugar group (2009)
  • Author(s): H. Saigusa, S. Urashima, H. Asami
  • Journal Title: Journal of Physical Chemistry A 113 Pages: 3455-3462
  • Peer Reviewed
• [Journal Article] Ultraviolet Spectroscopy and Theoretical Investigations of Mono-and Dihydrated Clusters of the Guanine Nucleosides. Different Hydration Structures for Guanosine and 2'-Deoxyguanosine (2008)
  • Author(s): H.Saigusa, N. Mizuno, H. Asami, K. Takahashi, M. Tachikawa
  • Journal Title: Bull.Chem.Soc.Jpn. 81 Pages: 1274-1281
  • Peer Reviewed
• [Journal Article] Ultraviolet spectroscopy and theoretical calculations of mono- and dihydrated clusters of the guanine nucleosides : Possibility of different hydration structures for guanosine and 2'-deoxyguanosine (2008)
  • Author(s): H. Saigusa, N. Mizuno, H. Asami, K. Takahashi, M. Tachikawa
  • Journal Title: Bulletin of Chemical Society of Japan 81 Pages: 1274-1281
  • NAID: 10024330971
  • Peer Reviewed
• [Presentation] Conformational analysis of the guanine nucleosides based on ab initio anharmonic vibrational calculation (2010)
  • Author(s): Poster, H.Asami, H.Saigusa, K.Yagi
  • Organizer: Pacifichem 2010 Congress (865220)[日本化学会のハイライト講演に採択]
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2010-12-18
• [Presentation] Effects of hydration on the base pair structures of guanine-Stacked vs. planar base pairing (2010)
  • Author(s): Poster, H.Asami, H.Saigusa
  • Organizer: Pacifichem 2010 Congress (872314)
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2010-12-17
• [Presentation] Radiationless decay of thymine and uracil : Difference in lifetimes between the two systems (2010)
  • Author(s): Poster, Y.Ito, A. Yamaguchi, S. Koseki, Y. Fujimura, H. Kono, N.Shimakura, H.Saigusa
  • Organizer: Pacifichem 2010 Congress (656)
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2010-12-17
• [Presentation] Effects of hydration on the base pair structures of guanine-Stacked vs.planar base pairing (2010)
  • Author(s): H.Asami, H.Saigusa
  • Organizer: Pacifichem 2010 Congress (872314)
  • Place of Presentation: Honolulu, Hawai
  • Year and Date: 2010-12-17
• [Presentation] Conformational analysis of the guanine nucleosides based on ab initio anharmonic vibrational calculation (2010)
  • Author(s): H.Asami, H.Saigusa, K.Yagi
  • Organizer: Pacifichem 2010 Congress (865220)
  • Place of Presentation: Honolulu, Hawai
  • Year and Date: 2010-12-17
• [Presentation] Microhydration of the guanine nucleosides and base pairs (2010)
  • Author(s): Contributed talk, H.Saigusa
  • Organizer: Pacifichem 2010 Congress (842124)
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2010-12-16
• [Presentation] Microhydration of the guanine nucleosides and base pairs (2010)
  • Author(s): H.Saigusa
  • Organizer: Pacifichem 2010 Congress (842124)
  • Place of Presentation: Honolulu, Hawai
  • Year and Date: 2010-12-16
• [Presentation] 不揮発性分子のレーザー蒸発/レーザー分光の装置開発 (2010)
  • Author(s): 吉川竜一, 片桐勇志, 江幡孝之, 井口佳哉, 三枝洋之
  • Organizer: 分子科学討論会2010(4A16)
  • Place of Presentation: 大阪大学
  • Year and Date: 2010-09-17
• [Presentation] 揮発性分子のレーザー蒸発/レーザー分光の装置開発 (2010)
  • Author(s): 吉川竜一, 片桐勇志, 江幡孝之, 井口佳哉, 三枝洋之
  • Organizer: 分子科学討論会2010(4A16)
  • Place of Presentation: 大阪大学
  • Year and Date: 2010-09-17
• [Presentation] グアニンヌクレオチドのリン酸基エステル化による非破壊的気化と赤外振動分光 (2010)
  • Author(s): 浅見祐也, 元田彩香, 塚本眞幸, 早川芳宏, 三枝洋之
  • Organizer: 分子科学討論会2010(3A01)
  • Place of Presentation: 大阪大学
  • Year and Date: 2010-09-16
• [Presentation] 赤外-紫外二重共鳴分光法によるグアニン-シトシン及びグアニン-グアニン塩基対の微細水和構造解析 (2010)
  • Author(s): 浦島周平, 浅見祐也, 三枝洋之
  • Organizer: 分子科学討論会2010(3A02)
  • Place of Presentation: 大阪大学
  • Year and Date: 2010-09-16
• [Presentation] 9-メチルグアニン一水和物の電子状態とその性質 (2010)
  • Author(s): 伊藤悠太, 浅見祐也, 三枝洋之, 小関史朗, 河野裕彦, 島倉紀之
  • Organizer: 分子科学討論会2010(3P097)
  • Place of Presentation: 大阪大学
  • Year and Date: 2010-09-16
• [Presentation] 9-メチルグアニン-水和物の電子状態とその性質 (2010)
  • Author(s): 伊藤悠太, 浅見祐也, 三枝洋之, 小関史朗, 河野裕彦, 島倉紀之
  • Organizer: 分子科学討論会2010(3P097)
  • Place of Presentation: 大阪大学
  • Year and Date: 2010-09-16
• [Presentation] Microscopic hydration of guanine nucleosides and base pairs (2010)
  • Author(s): Poster, H.Saigusa
  • Organizer: The 2010 Molecular and Ionic Clusters Conference
  • Place of Presentation: Niigata, Japan
  • Year and Date: 2010-09-07
• [Presentation] Intact laser desorption of guanosine 5'-monophosphate facilitated by the phosphoesterification : Conformational analysis by infrared vibrational spectroscopy (2010)
  • Author(s): Poster, H.Asami, A.Motoda, M.Tsukamoto, Y.Hayakawa, H.Saigusa
  • Organizer: The 2010 Molecular and Ionic Clusters Conference
  • Place of Presentation: Niigata, Japan
  • Year and Date: 2010-09-07
• [Presentation] Infrared Spectra of Hydrated Clusters of Guanine Nucleosides Observed by IR-UV Double Resonance Spectroscopy (2010)
  • Author(s): Oral, S.Urashima, H.Asami, H.Saigusa
  • Organizer: International Symposium on Molecular Spectroscopy 65th Meeting (TB05)
  • Place of Presentation: Columbus, Ohio
  • Year and Date: 2010-06-22
• [Presentation] Infrared Spectra of Hydrated Clusters of Guanine Nucleossides Observed by IR-UV Double Resonance Spectroscopy (2010)
  • Author(s): S.Urashima, H., Asami, H.Saigusa
  • Organizer: International Symposium on Molecular Spectroscopy 65th Meeting (TB05)
  • Place of Presentation: Columbus, Ohio
  • Year and Date: 2010-06-22
• [Presentation] Microscopic hydration structure of biological molecules-In connection with their functions (2010)
  • Author(s): Invited talk, H.Saigusa
  • Organizer: Symposium on Hydration Dynamics and Molecular Processes
  • Place of Presentation: Riken, Japan
  • Year and Date: 2010-03-16
• [Presentation] Non-destructive vaporization of GMP enabled by the phosphoesterification : Conformational preference in the gas phase (2010)
  • Author(s): Poster, H.Asami, A.Motoda, M.Tsukamoto, Y.Hayakawa, H.Saigusa
  • Organizer: 37th International Symposium on Nucleic Acid Chemistry
  • Place of Presentation: Yokohama, Japan
• [Presentation] DNA塩基グアニンの電子励起ダイナミクスに及ぼす水和の影響 (2009)
  • Author(s): 浅見祐也, 浦島周平, 三枝洋之
  • Organizer: 分子科学討論会2009(4P010)
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-24
• [Presentation] DNA塩基グアニンの電子励起ダイナミクスに及ぼす水和の影響 (2009)
  • Author(s): 浅見裕也, 浦島周平, 三枝洋之
  • Organizer: 第3回分子科学討論会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-24
• [Presentation] レーザー蒸発/真空紫外イオン化を用いた不揮発分の質量分析法の開発 (2009)
  • Author(s): 吉川竜一, 江幡孝之, 井口佳哉, 三枝洋之
  • Organizer: 分子科学討論会2009(2P017)
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-22
• [Presentation] 赤外振動スペクトルとab initio非調和振動計算によるグアニンヌクレオシドの構造解析 (2009)
  • Author(s): 浅見祐也, 三枝洋之, 八木清
  • Organizer: 分子科学討論会2009(1A18)
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-21
• [Presentation] 紫外-赤外二重共鳴法による2'-デオキシグアノシンの微細水和構造の決定:グアノシンとの比較 (2009)
  • Author(s): 浦島周平, 浅見祐也, 三枝洋之
  • Organizer: 分子科学討論会2009(1A19)
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-21
• [Presentation] 赤外振動スペクトルと ab initio 非調和振動計算によるグアニンヌクレオシドの構造解析 (2009)
  • Author(s): 浅見祐也, 三枝洋之, 八木清
  • Organizer: 第3回分子科学討論会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-21
• [Presentation] 紫外-赤外二重共鳴法による2'-デオキシグアノシンの微細水和構造の決定 : グアノシンとの比較 (2009)
  • Author(s): 浦島周平, 浅見祐也, 三枝洋之
  • Organizer: 第3回分子科学討論会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-21
• [Presentation] Intact vaporization of biological molecules by laser desorption and its applications to laser spectroscopic studies (2009)
  • Author(s): 三枝洋之
  • Organizer: 特定領域「高次系分子科学」国際シンポジウム
  • Place of Presentation: 東京工業大学(大岡山)
  • Year and Date: 2009-06-05
• [Presentation] Excited-state dynamics of nucleic acid bases-Bright and dark sides (2009)
  • Author(s): Contributed talk, H.Saigusa
  • Organizer: 25^<th> Conference on Chemical Reaction Dynamics(2E09)
  • Place of Presentation: Ohmiya, Japan
  • Year and Date: 2009-06-02
• [Presentation] Excited-state dynamics of nucleic acid bases -Bright and dark sides (Invited talk) (2009)
  • Author(s): 三枝洋之
  • Organizer: 第25回化学反応討論会
  • Place of Presentation: 大宮ソニックシティ
  • Year and Date: 2009-06-02
• [Presentation] グアニンヌクレオシド水和クラスターの電子スペクトルにみられる異性体構造 (2008)
  • Author(s): 浅見裕也、浦島周平、三枝洋之
  • Organizer: 分子科学討論会2008(3B02)
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-09-26
• [Presentation] 4つの核酸塩基ヌクレオシドに対する安定水和構造の比較 (2008)
  • Author(s): 浦島周平、浅見裕也、三枝洋之
  • Organizer: 分子科学討論会2008(1P061)
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-09-26
• [Presentation] グアニンヌクレオシド水和クラスターの電子スペクトルにみられる異性体構造 (2008)
  • Author(s): 浅見裕也, 浦島周平, 三枝洋之
  • Organizer: 第2回分子科学討論会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-09-26
• [Presentation] 4つの核酸塩基ヌクレオシドに対する安定水和構造の比較 (2008)
  • Author(s): 浦島周平, 浅見裕也, 三枝洋之
  • Organizer: 第2回分子科学討論会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-09-24
• [Presentation] 核酸塩基ヌクレオシドの微視的水和構造の理論的解明 (2008)
  • Author(s): 浅見裕也、浦島周平、三枝洋之
  • Organizer: 理論化学討論会2008(1P26)
  • Place of Presentation: 慶応大学
  • Year and Date: 2008-05-22
• [Presentation] 核酸塩基ヌクレオシドの微視的水和構造の理論的解明 (2008)
  • Author(s): 浅見裕也, 浦島周平, 三枝洋之
  • Organizer: 第11回理論化学討論会
  • Place of Presentation: 慶応大学
  • Year and Date: 2008-05-22
• [Presentation] 生体分子及びそのクラスターのレーザー脱離-超音速分子線分光 (2008)
  • Author(s): 三枝洋之
  • Organizer: 第8回分子分光研究会
  • Place of Presentation: 神戸大学(招待講演)
  • Year and Date: 2008-05-17
• [Presentation] 生体分子及びそのクラスターのレーザー脱離-超音速分子線分光 (2008)
  • Author(s): 三枝 洋之
  • Organizer: 第8回分子分光研究会(招待講演)
  • Place of Presentation: 神戸大学
  • Year and Date: 2008-05-17
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