Approach to Molecular Development of the Drug that induces Mitotic Catastrophe in Cancer cells

• Project/Area Number: 20590391
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Hokkaido University
• Principal Investigator: TAKIMOTO Masato 北海道大学, 遺伝子病制御研究所, 准教授 (30179585)
• Co-Investigator(Kenkyū-buntansha): OCHIYA Takahiro 国立がんセンター研究所, 分子細胞治療研究分野, 分野長 (60192530)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 分裂破局死 / D40 / がん / 細胞死 / 増殖抑制 / RNA干渉 / p53 / D40siRNA / がん細胞 / p53 nul 細胞 / D40,癌 / 分子創薬 / 癌細胞 / 分子標的

Research Abstract

Synthetic double-stranded RNA specific to D40 gene (D40 siRNA) human cancer cell lines. D40 siRNA caused a p53 independent cell death "Mitotic Catastrophe". Further D40 siRNA treatment to tumor-burden experimental animals induced inhibitions of the tumor growth. The molecular therapy targeting to D40 protein is encouraging in human cancer treatment in future.

Research Products

• [Journal Article] Testis cancer gene D40 expression and its relationship with clinicopathological features in infertile men (2011)
  • Author(s): Takumi Sasao Masato Takimoto Naoki Itoh Toshihiro Maeda Toshiaki Tanaka Naoya Masumori and Taiji Tsukamoto
  • Journal Title: International Journal of Urology Volume: 18 Issue: 2 Pages: 175-179
  • DOI: 10.1111/j.1442-2042.2010.02692.x
  • NAID: 10030821983
  • Peer Reviewed
• [Journal Article] Testis cancer gene D40 expression and its relationship with clinicopathological features in infertile men (2011)
  • Author(s): Takumi Sasao, Masato Takimoto, Naoki Itoh, et al.
  • Journal Title: Intl. J. of Urology 18 Pages: 175-179
  • NAID: 10030821983
  • Peer Reviewed
• [Journal Article] Testis cancer gene D40 expression and its relationship with clinicopatholoaical features in infertile men (2011)
  • Author(s): T. Sasao, et al.
  • Journal Title: International Journal of Urology Volume: 18 Pages: 175-179
  • Peer Reviewed
• [Journal Article] The Involvement of c-Abl an D40 (AF15q14/CASC5) protein in the regulation and cell proliferation in cancer (2008)
  • Author(s): Bogdanov K. and Masato Takimoto
  • Journal Title: Cell and Tissue Biology Volume: 2(4) Issue: 4 Pages: 354-359
  • DOI: 10.1134/s1990519x08040020
  • Peer Reviewed
• [Journal Article] Involvement of c-Abl and D40(AF15q14/CASC5) Protein in the Regulation of cell Proliferation and Cancer (2008)
  • Author(s): Konstantin Bogdanov, Masato Takimoto
  • Journal Title: Cell and Tissue Biology 208,2 Pages: 354-359
  • Peer Reviewed
• [Journal Article] The Involvement of c-Abl an D40 (AF15q14/CASC5) protein in the regulation and cell proliferation in cancer (2008)
  • Author(s): K. Bogdanov & M. Takimoto
  • Journal Title: Cell and Tissue Biology Volume: 2 Pages: 354-359
  • Peer Reviewed
• [Presentation] Analyses on the mechanism of the cell death ofhuman cancer cell line induced by shortinhibitory RNA specific to D40 gene (2011)
  • Author(s): Masato Takimoto Hiroki Tanaka Urata Yuri
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-12-16
• [Presentation] Analyses on the mechanism of the cell deatn of human cancer cell line induced by short inhibitory RNA specific to D40 gene (2011)
  • Author(s): 瀧本将人, 他
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-12-16
• [Presentation] Apoptotic Cell Death and Growth Inhibition of Human Cancer Cell line by Depletion of D40 protein expression with siRNA (2010)
  • Author(s): Masato Takimoto Yuri N. Urata
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪リーガロイヤルホテル(大阪)
  • Year and Date: 2010-09-22
• [Presentation] Apoptotic Cell Death and Growth Inhibition of Human Cancer Cell line by Depletion of D40 protein expression with siRNA. (2010)
  • Author(s): M. Takimoto
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 大阪リーガロイヤルホテル
  • Year and Date: 2010-09-22
• [Presentation] Pre-acrosomal outer membrane-associating protein D40 is overexpressed in human cancers (2010)
  • Author(s): Masato Takimoto Takumi Sasao Naoki Itoh Hiroko Takano Satoshi Watanabe Gang Wei Peizhong Mao Taiji Tsukamoto
  • Organizer: 第30回札幌国際がんシンポジウム
  • Place of Presentation: 北海道大学学術交流会館(札幌)
  • Year and Date: 2010-06-28
• [Presentation] Pre-acrosomal outer membrane-associating protein D40 is overexpressed in human cancers (2010)
  • Author(s): M. Takimoto, T. Sasao, et al.
  • Organizer: 第30回札幌国際がんシンポジウム
  • Place of Presentation: 札幌
• [Presentation] Apoptotic Cell Death and Growth Inhibition Of Human Cancer Cell line by Depletion of D40 Protein expression with siRNA (2010)
  • Author(s): M. Takimoto, Y.N. Urata.
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
• [Presentation] Growth inhibition and Apoptotic Cell death of Cancer cells by Inhibition of D40 protein expression (2009)
  • Author(s): 瀧本将人
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜市、横浜パシフィコ
  • Year and Date: 2009-12-12
• [Presentation] Growth inhibition and Apoptotic Cell death of Cancer cells by Inhibition of D40 protein expression (2009)
  • Author(s): Urata Yuri Masato Takimoto, 他2名
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜)
• [Presentation] Growth inhibition and Apoptotic Cell death of Cancer cells by Inhibition of D40 protein expression (2009)
  • Author(s): Y.N. Urata, M. Takimoto, 他2名
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Induction of cell death and growth inhibition of cancer cell by the inhibition of expression of D40/AF15q14 gene product (2008)
  • Author(s): 瀧本将人
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋市名古屋国際会議場
  • Year and Date: 2008-10-28
• [Presentation] D40/AF14q15遺伝子産物の発現抑制による癌細胞の細胞死の誘導と増殖抑制(躍動する分子生物学ー北の大地から) (2008)
  • Author(s): 瀧本将人、Yuri Urata、田中宏樹、Konstantin Bogdanov、高岡晃教
  • Organizer: 日本分子生物学会第8回春期シンポジウム
  • Place of Presentation: 京王プラザホテル(札幌)
  • Year and Date: 2008-05-26
• [Presentation] D40/AF14q15遺伝子産物の発現抑制による癌細胞の細胞死の誘導と増殖抑制 (2008)
  • Author(s): 瀧本将人
  • Organizer: 日本分子生物学会第8回春期シンポジウム
  • Place of Presentation: 札幌市京王プラザホテル札幌
  • Year and Date: 2008-05-26
• [Presentation] Induction of cell death and growth inhibition of cancer cell by the inhibition of expression of D40/AF15q14 gene product (2008)
  • Author(s): Masato Takimoto, et al.
  • Place of Presentation: 名古屋国際会議場(名古屋)
• [Presentation] Induction of cell death and growth inhibition of cancer cell by the inhibition of expression of D40/AF15q14 gene product (2008)
  • Author(s): M. Takimoto, et al.
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] D40/AF14q15遺伝子産物の発現抑制による癌細胞の細胞死の誘導と増殖抑制 (2008)
  • Author(s): 瀧本将人, 他4名
  • Organizer: 日本分子生物学会第8回春期シンポジウム
  • Place of Presentation: 札幌
• [Patent(Industrial Property Rights)] D40あるいはCASC5、又は、該癌・精巣抗原タンパク質のスプライシングアイソフォームタンパク質をコードする遺伝子、及び、該遺伝子産物をターゲットとした癌細胞の増殖・分裂阻止及び細胞死の誘導方法、及び、該増殖・分裂阻止及び細胞死を誘導する物質のスクリーニング方法 (2011)
  • Inventor(s): 瀧本将人、ユリウラタ、葛巻暹
  • Industrial Property Rights Holder: 北海道大学
  • Acquisition Date: 2011-10-28
• [Patent(Industrial Property Rights)] 新規ヒト癌・精巣抗原及びその遺伝子 (2010)
  • Inventor(s): 瀧本将人、葛巻暹、佐藤昇志、佐原弘益
  • Industrial Property Rights Holder: 科学技術振興財団
  • Acquisition Date: 2010-04-30
• [Patent(Industrial Property Rights)] 新規ヒト癌・精巣抗原及びその遺伝子 (2010)
  • Inventor(s): 瀧本将人、葛巻暹、佐藤昇志、佐原弘益
  • Industrial Property Rights Holder: 科学技術振興財団
  • Filing Date: 2010-04-30
• [Patent(Industrial Property Rights)] D40あるいはCASC5、又は、該癌・精巣抗原タンパク質のスプライシングアイソフォームタンパク質をコードする遺伝子、及び、該遺伝子産物をターゲットとした癌細胞の増殖・分裂阻止及び細胞死の誘導方法、及び、該増殖・分裂阻止及び細胞死を誘導する物質のスクリーニング方法 (2007)
  • Inventor(s): 瀧本将人、ユリウラタ、葛巻暹
  • Industrial Property Rights Holder: 北海道大学
  • Industrial Property Number: 2005-303676
  • Filing Date: 2007-05-10