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Elucidation of the mechanism for radioresistance from the viewpoint of gap junction using 3D-cultured cancer cells

Research Project

Project/Area Number 20591500
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionYokohama City University

Principal Investigator

TORIGOE Soichiro  横浜市立大学, 医学研究科, 客員研究員 (20264658)

Co-Investigator(Kenkyū-buntansha) MITSUDOU Kenji  横浜市立大学, 医学部, 准教授 (70303641)
CHIKUMARU Hiroshi  横浜市立大学, 医学部, 助教 (80217231)
OHTA Shinsuke  横浜市立大学, 医学研究科, 客員研究員 (30336581)
ADACHI Makoto  横浜市立大学, 附属病院, 指導診療医 (10468192)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsがん / 培養細胞 / ギャップ結合 / 放射線 / 抵抗性 / 3次元培養細胞 / 放射線感受性 / メカニズム / ギャップ結合阻害 / lindane / scrape loading assay / コロニー形成法 / RT-PCR / Immunoblot
Research Abstract

Since both HSC4 and CAL27, head and neck cancer cell lines, expressed connexin43 mRNA but exhibit no expression at protein level, hypofunction of gap junction was speculated. In another experiment, OSC-19 and HSC-3 cell lines showed decreased IR sensitivity under hypoxic culture condition, but no significant change in connexin43 protein. No obvious relationship was revealed between connexin43 and IR sensitivity in used cell lines.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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