The study of the tumorigenic and tumor related genes for the pediatric solid tumors, especially hepatoblastoma

• Project/Area Number: 20592092
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatric surgery
• Research Institution: Nihon University
• Principal Investigator: IKEDA Taro (2009-2010) Nihon University, 医学部, 助教 (00318396); 草深 竹志 (2008) Nihon University, 医学部, 教授 (70263267)
• Co-Investigator(Kenkyū-buntansha): SUGITO Kiminobu 日本大学, 医学部, 助教 (10328750) ; NAGASE Hiroki 日本大学, 医学部, 客員教授 (90322073) ; FURUYA Takeshi 日本大学, 医学部, 専修医 (20568539) ; 池田 太郎 日本大学, 医学部, 助教 (00318396)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 小児固形腫瘍 / DNAメチル化 / 癌関連遺伝子 / MassARRAY / MassARRAY epiTYPER法 / MassARRAY epityper法

Research Abstract

We have identified that at least four (SLC16A、ZNF206、NR4A3、ZAR-1) out of 12 tDMRs showed frequent aberrant methylation in pediatric solid tumors. The methylation of these four genes confers novel candidate pediatric solid tumors-related epigenetic factors. SLC16A, ZNF206, and NR4A3 genes were related to the prognosis in neuroblastoma. Hypermethylation of the ZAR-1 non-promoter is extremely frequent in hepatoblastoma, and ZAR-1 expression plays a tumorigenic role. The pathway of these four genes was different. It could be useful the treatment of children with pediatric solid tumors to analyze the methylation level of four genes (SLC16A、ZNF206、NR4A3、ZAR-1).

Research Products

• [Journal Article] The clinical course in pediatric solid tumor patients with focal nodular hyperplasia of the liver. (2011)
  • Author(s): Sugito K, Uekusa S, Kawashima H, Furuya T, Ohashi K, Inoue M, Ikeda T, Koshinaga T, Tomita R, Mugishima H, Maebayashi T
  • Journal Title: Int J Clin Oncol. (Epub ahead of print)
  • NAID: 10030246642
  • Peer Reviewed
• [Journal Article] Mesenchymal hamartoma of the liver originating in the caudate lobe with t(11;19)(q13;q13.4) : Report of a case. (2010)
  • Author(s): Sugito K, Kawashima H, Uekusa S, Inoue M, Ikeda T, Kusafuka T
  • Journal Title: Surgery Today 40 Pages: 83-87
  • Peer Reviewed
• [Journal Article] Application of Radiofrequency Ablation for Giant Solid Pseudopapillary Tumor of the Pancreas. (2010)
  • Author(s): Sugito K, Kusafuka T, Hoshino M, Inoue M, Ikeda T, Hagiwara N, Koshinaga T, Shichino H, Chin M, Mugishima H
  • Journal Title: Pediatrics International 52
  • Peer Reviewed
• [Journal Article] 完全寛解後に緩徐な血清AFP値の上昇に伴って再発肺転移を認めた肝芽腫の1例 (2010)
  • Author(s): 杉藤公信,斉藤玲奈,花田学,吉澤信輔,植草省太,川島弘之,古屋武史,大橋研介,井上幹也,池田太郎,越永従道,草深竹志,梁尚弘,谷ヶ崎博,七野浩之,陳基明,麦島秀雄
  • Journal Title: 小児がん 47 Pages: 132-136
  • NAID: 110007575330
  • Peer Reviewed
• [Journal Article] 膵芽腫・膵腺房細胞癌の混合型腫瘍の1例 (2010)
  • Author(s): 杉藤公信,大橋研介,吉澤信輔,植草省太,川島弘之,古屋武史,井上幹也,池田太郎,越永従道,草深竹志,谷ヶ崎博,七野浩之,陳基明,麦島秀雄
  • Journal Title: 小児がん 47 Pages: 137-141
  • NAID: 110007575331
  • Peer Reviewed
• [Journal Article] Usefulness of power Doppler ultrasonography and superparamagnetic iron oxide enhanced magnetic resonance imaging for diagnosis of focal nodular hyperplasia of the liver after treatment of neuroblastoma. (2010)
  • Author(s): Sugito K, Kusafuka T, Kawashima H, Uekusa S, Furuya T, Ohashi K, Inoue M, Ikeda T, Koshinaga T, Maebayashi T
  • Journal Title: Pediatr Hematol Oncol. 27 Pages: 250-6
  • Peer Reviewed
• [Journal Article] von Hippel Lindau (VHL)遺伝子の生殖細胞変異を伴った傍神経節腫の1例 (2010)
  • Author(s): 川島弘之,杉藤公信,吉澤信輔,植草省太,古屋武史,大橋研介,池田太郎,越永従道,草深竹志,永瀬浩喜
  • Journal Title: 日本小児外科学会誌 46 Pages: 777-782
  • NAID: 110007657530
  • Peer Reviewed
• [Journal Article] Application of radiofrequency ablation for giant solid pseudopapillary tumor of the pancreas (2010)
  • Author(s): Sugito K
  • Journal Title: Pediatr Int. Volume: 52
  • Peer Reviewed
• [Journal Article] Mesenchymal hamartoma of the liver originating in the caudate lobe with t(11;19)(q13;q13.4) : report of a case. (2010)
  • Author(s): Sugito K
  • Journal Title: Surg Today Volume: 40 Pages: 83-87
  • Peer Reviewed
• [Journal Article] Usefulness of power Doppler ultrasonography and superparamagnetic iron oxide enhanced magnetic resonance imaging for diagnosis of focal nodular hyperplasia of the liver after treatment of neuroblastoma (2010)
  • Author(s): Sugito K
  • Journal Title: Pediatr Hematol Oncol. Volume: 27 Pages: 250-6
  • Peer Reviewed
• [Journal Article] 腎腫瘍(腎芽腫と腎細胞癌) (2009)
  • Author(s): 杉藤公信,川島弘之,池田太郎,越永従道,草深竹志
  • Journal Title: 小児外科 41 Pages: 1118-1123
• [Journal Article] 【知っておきたい泌尿器科の知識 その病態と治療】腎腫瘍 腎芽腫と腎細胞癌 (2009)
  • Author(s): 杉藤公信
  • Journal Title: 小児外科 41巻 Pages: 1118-1123
• [Journal Article] Usefulness of color doppler sonography and 99m Tc-RBC scintigraphy for preoperative diagnosis of a venous malformation of the small intestine in a 2-year-old child. (2008)
  • Author(s): Kiminobu Sugito Takeshi Kusafuka
  • Journal Title: Journal of Clinical Ultrasound 36 Pages: 56-58
  • Peer Reviewed
• [Presentation] 神経芽腫におけるNR4A3遺伝子のDNA低メチル化と予後 (2010)
  • Author(s): 川島弘之,杉藤公信,吉澤信輔,植草省太,古屋武史,池田太郎,越永従道,永瀬浩喜
  • Organizer: 第26回日本小児がん学会
  • Place of Presentation: 大阪
  • Year and Date: 2010-12-18
• [Presentation] INHIBITION OF MYCN GENE TRANSCRIPTION BY PYRROLE-IMIDAZOLE POLYAMIDE IN NEUROBLASTOMA CELLS. (2010)
  • Author(s): Uekusa S, Sugito K, Kawashima H, Furuya T, Inoue M, Ikeda T, Koshinaga T, Nagase H
  • Organizer: 42th International Society of Paediatric Oncology
  • Place of Presentation: Boston
  • Year and Date: 2010-10-23
• [Presentation] EXON5CPG ISLAND HYPOMETHYLATION OF THE ZNF206 GENE, ASSOSICATED WITH NEURONAL DIFFERENTIATION IN MICE AND DEVLOPMENT OF NEUROBLASTOMA IN HUMAN. (2010)
  • Author(s): Kawashima H, Sugito K, Uekusa S, Furuya T, Muroi S, Igarashi J, Ikeda T, Koshinaga T, Nagase H
  • Organizer: 42th International Society of Paediatric Oncology
  • Place of Presentation: Boston
  • Year and Date: 2010-10-23
• [Presentation] FOCAL NODULAR HYPERPLASIA OF THE LIVER IN PEDIATRIC SOLID TUMOR PATIENTS : WHAT ARE THE RISK FACTORS? (2010)
  • Author(s): Sugito K, Yoshizawa S, Uekusa S, Kawashima H, Furuya T, Ohashi K, Inoue M, Ikeda T, Koshinaga T, Maebayashi T
  • Organizer: 42th International Society of Paediatric Oncology
  • Place of Presentation: Boston
  • Year and Date: 2010-10-23
• [Presentation] DNA HYPOMETHYLATION LEVEL OF THE CPG ISLAND REGION IN THE ZNF206-EXONS IS ASSOSICATED WITH MICE NEURONAL DEVELOPMENT AND DEVELOPMENT OF HUMAN NEUROBLASTOMAS. (2010)
  • Author(s): Kawashima H
  • Organizer: 42th International Society of Paediatric Oncology
  • Place of Presentation: Boston
  • Year and Date: 2010-10-23
• [Presentation] MYCN遺伝子を標的としたピロールイミダゾール(PI)ポリアミドによる神経芽腫細胞の抗腫瘍効果の検討 (2010)
  • Author(s): 植草省太,杉藤公信,吉澤信輔,川島弘之,古屋武史,益子貴行,大橋研介,井上幹也,池田太郎,越永従道,永瀬浩喜
  • Organizer: 第47回日本小児外科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-06-19
• [Presentation] 神経芽腫におけるNR4A3遺伝子のメチル化解析 (2010)
  • Author(s): 川島弘之,杉藤公信,植草省太,古屋武史,池田太郎,越永従道,永瀬浩喜
  • Organizer: 第47回日本小児外科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-06-18
• [Presentation] MYCN遺伝子を標的としたピロールイミダゾール(PI)ポリアミドによる神経芽腫細胞の抗腫瘍効果の検討 (2010)
  • Author(s): 植草省太,杉藤公信,吉澤信輔,川島弘之,古屋武史,益子貴行,大橋研介,井上幹也,池田太郎,越永従道,草深竹志,永瀬浩喜
  • Organizer: 第110回日本外科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-09
• [Presentation] 神経芽腫におけるZNF206遺伝子のメチル化解析 (2010)
  • Author(s): 川島弘之,杉藤公信,植草省太,古屋武史,池田太郎,越永従道,草深竹志,永瀬浩喜
  • Organizer: 第110回日本外科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-04-09
• [Presentation] 神経芽腫における新規予後因子,癌関連遺伝子の探索 (2009)
  • Author(s): 川島弘之,杉藤公信,植草省太,古屋武史,大橋研介,池田太郎,越永従道,草深竹志,麦島秀雄,永瀬浩喜
  • Organizer: 第25回日本小児がん学会
  • Place of Presentation: 千葉
  • Year and Date: 2009-11-27
• [Presentation] 神経芽腫,肝芽腫における5D52領域のメチル化異常の検討 (2009)
  • Author(s): 植草省太,杉藤公信,川島弘之,吉澤信輔,古屋武史,大橋研介,池田太郎,越永従道,草深竹志,麦島秀雄,永瀬浩喜
  • Organizer: 第25回日本小児がん学会
  • Place of Presentation: 千葉
  • Year and Date: 2009-11-27
• [Presentation] MYCN遺伝子を標的としたピロールイミダゾール(PI)ポリアミドによる神経芽腫細胞の抗腫瘍効果の検討 (2009)
  • Author(s): 植草省太,杉藤公信,川島弘之,古屋武史,草深竹志,永瀬浩喜
  • Organizer: 第13回神経芽腫研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-08-22
• [Presentation] 神経芽腫におけるSLC16A5遺伝子promoter領域のメチル化の検討 (2009)
  • Author(s): 川島弘之,杉藤公信,植草省太,池田太郎,越永従道,永瀬浩喜
  • Organizer: 第46回日本小児外科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-06-03
• [Presentation] 神経芽腫における新規予後因子、癌関連遺伝子の探索 (2009)
  • Author(s): 川島弘之
  • Organizer: 第46回 日本小児外科学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2009-06-03
• [Presentation] A von Hippel-Lindau gene mutation in pediatric paraganglioma : possible involvement of its disease pathogenesis. (2009)
  • Author(s): Kawashima H, Sugito K, Uekusa S, Inoue M, Ikeda T, Hagiwara N, Koshinaga T, Kusafuka T, Nagase H
  • Organizer: 42th Pacific Association of Pediatric Surgeons
  • Place of Presentation: Hong Kong
  • Year and Date: 2009-05-14
• [Presentation] Frequent SLC16A5 promoter methylation in progressiveneuroblastomas (2008)
  • Author(s): Hiroyuki Kawashima Takeshi Kusafuka
  • Organizer: Advances in Neuroblastoma Research2008
  • Place of Presentation: Makuhari Messe Chiba Japan
  • Year and Date: 2008-05-22
• [Book] 【神経芽腫マススクリーニングの今後】マススクリーニング陽性でその後の無治療期間を経て腫瘍進展が考えられた症例 (2008)
  • Author(s): 杉藤 公信, 草深 竹志
  • Publisher: 小児外科