Regulation of host-alternative splicing in herpesvirus-infected

• Project/Area Number: 20790353
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: NOJIMA Takayuki Tokyo Medical and Dental University, 大学院・疾患生命科学研究部, 特任助教 (80431956)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 単純ヘルペスウイルス / 選択的スプライシング / 前骨髄球性白血病(PML)遺伝子 / ICP27 / Pre-Mrna / スプライシングアイソフォーム / ミニ遺伝子 / Pre-mRNA

Research Abstract

Herpes simplex virus type-2 (HSV-2) is one of DNA viruses, which is spread world-wide and replicates own virus genome in host-cell nucleus. In this study, I examined how HSV-2 replicates own genome in the nucleus. Here I showed that HSV-2 virus protein interfered with the step of host mRNA processing. I further showed that this interference played important role of virus replication.

Research Products

• [Journal Article] Herpesvirus protein ICP27 switches PML isoform by altering mRNA splicing (2009)
  • Author(s): Nojima T, Oshiro-Ideue T, Nakanoya T, Kawamura H, Morimoto T, Kawaguchi Y, Kataoka N, Hagiwara M
  • Journal Title: Nucleic Acids Research 37 Pages: 6515-6527
  • Peer Reviewed
• [Journal Article] Herpesvirus protein ICP27 switches PML isoform by altering mRNA splicing (2009)
  • Author(s): Takayuki Nojima
  • Journal Title: Nucleic Acids Research 37 Pages: 6515-6527
  • Peer Reviewed
• [Presentation] HSV-2増殖に関わるPMLアイソフォーム発現制御 (2009)
  • Author(s): 野島孝之
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2009-10-21
• [Presentation] Herpesvirus protein ICP27 switches PML isoform by altering mRNA Splicing (2009)
  • Author(s): Takayuki Nojima
  • Organizer: RNA meeting 2009
  • Place of Presentation: Wisconsin University, USA
  • Year and Date: 2009-05-26
• [Presentation] Herpesvirus protein ICP27 switches PAM isoform by altering mRNA splicing (2009)
  • Author(s): 野島孝之
  • Organizer: RNA meeting 2009
  • Place of Presentation: Wisconsin University, USA
  • Year and Date: 2009-05-26
• [Presentation] ウイルス感染が引き起こすPMLアイソフォームの切り換え (2008)
  • Author(s): 野島孝之
  • Organizer: 第10回日本RNAミーティング
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-07-23
• [Presentation] ウイルス感染が引き起こすPMLアイソフォームの切り換え (2008)
  • Author(s): 野島孝之
  • Organizer: 第10回 日本RNAミーティング
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2008-07-23
• [Book] RNAスプライシングの可視化による創薬スクリーニング (2010)
  • Author(s): 野島孝之, 萩原正敏
  • Total Pages: 7
  • Publisher: 秀潤社
• [Book] 細胞工学(特集)RNAスプライシングの可視化による創薬スクリーニング (2010)
  • Author(s): 野島孝之
  • Total Pages: 7
  • Publisher: 秀潤社
• [Book] ウイルスによるスプライシング暗号の利用と撹乱 (2009)
  • Author(s): 野島孝之, 萩原正敏
  • Total Pages: 6
  • Publisher: 羊土社
• [Book] 実験医学(増刊)ウイルスによるスプライシング暗号の利用と撹乱 (2009)
  • Author(s): 野島孝之
  • Total Pages: 6
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.tmd.ac.jp/mri/mri-end/index.html
• [Remarks]
  • URL: http://www.tmd.ac.jp/mri/mri-end/index.html
• [Remarks]
  • URL: http://www.tmd.ac.jp/mri/mri-end/index.html