Bone regeneration procedure with tongue muscle-derived stem cell
Project/Area Number |
21390540
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Surgical dentistry
|
Research Institution | Yamaguchi University |
Principal Investigator |
UEYAMA Yoshiya 山口大学, 大学院・医学系研究科, 教授 (00168668)
|
Co-Investigator(Kenkyū-buntansha) |
HARADA Koji 山口大学, 大学院・医学系研究科, 助教 (60253217)
ITASHIKI Yasutaka 山口大学, 医学部附属病院, 医員 (70530236)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2009: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
|
Keywords | マウス舌筋由来幹細胞 / 分化誘導 / 骨再生 / β-TCPゼラチンスポンジ / 骨芽細胞 |
Research Abstract |
Tongue muscle-derived Sca-1 positive cells(TDSCs) were isolated from Jcl-ICR mice tongue muscle by magnetic cell separation system with microbeads. TDSCs differentiated to osteoblast lineage demonstrated by up-regulation of osteoblastic makers and formation of mineralized matrix with alizarin red or Von Kossa positive in vitro. The bone formation was observed in the gelatin sponges ofβ-TCP. These data suggest that TDSCs retain its osteogenic differentiation potential, and may be useful for regenerative medicine of bone.
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Report
(4 results)
Research Products
(5 results)