Bone regeneration procedure with tongue muscle-derived stem cell

• Project/Area Number: 21390540
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Yamaguchi University
• Principal Investigator: UEYAMA Yoshiya 山口大学, 大学院・医学系研究科, 教授 (00168668)
• Co-Investigator(Kenkyū-buntansha): HARADA Koji 山口大学, 大学院・医学系研究科, 助教 (60253217) ; ITASHIKI Yasutaka 山口大学, 医学部附属病院, 医員 (70530236)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2009: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: マウス舌筋由来幹細胞 / 分化誘導 / 骨再生 / β-TCPゼラチンスポンジ / 骨芽細胞

Research Abstract

Tongue muscle-derived Sca-1 positive cells(TDSCs) were isolated from Jcl-ICR mice tongue muscle by magnetic cell separation system with microbeads. TDSCs differentiated to osteoblast lineage demonstrated by up-regulation of osteoblastic makers and formation of mineralized matrix with alizarin red or Von Kossa positive in vitro. The bone formation was observed in the gelatin sponges ofβ-TCP. These data suggest that TDSCs retain its osteogenic differentiation potential, and may be useful for regenerative medicine of bone.

Research Products

• [Presentation] Basic investigation of regenerative therapy for bone using Sca-1 positive cells derived from mouse tongue muscle (2010)
  • Author(s): Koji Harada, Yasutaka Itashiki, Takanori Takenawa, Toyoko Harada, Yoshiya Ueyama
  • Organizer: 20^<th> Congress of the European Association for Cranio-Maxillo-Facial Surgery
  • Place of Presentation: Bruges, Belgium
  • Year and Date: 2010-09-14
• [Presentation] Basic investigation of regenerative therapy for bone using Sca-1 positive cells derived from mouse tongue muscle (2010)
  • Author(s): Koji Harada
  • Organizer: 20th Congress of the European Association for Cranio-Maxillo-Facial Surgery
  • Place of Presentation: Bruges, Belgium
  • Year and Date: 2010-09-14
• [Presentation] マウス舌筋由来幹細胞を用いた骨再生医療に関する基礎的研究 (2010)
  • Author(s): 板敷康隆, 原田耕志, 竹縄隆徳, 加藤芳明.上山吉哉
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島国際会議場,広島
  • Year and Date: 2010-03-19
• [Presentation] マウス舌筋由来幹細胞を用いた骨再生医療に関する基礎的研究 (2010)
  • Author(s): 板敷康隆
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島国際会議場(広島県)
  • Year and Date: 2010-03-19
• [Presentation] マウス舌筋由来幹細胞を用いた骨再生医療に関する基礎的研究 (2009)
  • Author(s): 原田耕志, 板敷康隆, 竹縄隆徳, 加藤芳明.上山吉哉
  • Organizer: 第56回日本口腔外科学会総会
  • Place of Presentation: 札幌コンベンションセンター,札幌
  • Year and Date: 2009-10-11