| Project/Area Number |
21591127
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | University of Yamanashi |
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Principal Investigator |
ENDO Toyoshi 山梨大学, 大学院・医学工学総合研究部, 准教授 (00152017)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Tetsuro 山梨大学, 大学院・医学工学総合研究部, 教授 (30113442)
KANESHIGE Masahiro 山梨大学, 医学部附属病院, 助教 (20377518)
TAKIZAWA Souichi 山梨大学, 大学院・医学工学総合研究部, 助教 (80456467)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | エネルギー / 糖質代謝異常 / 1型糖尿病 / 自己免疫性膵炎 / アミラーゼ / 自己抗体 / 劇症1型糖尿 / ELISA / IgG4 / 劇症1型糖尿病 |
|---|
| Research Abstract |
Fulminant type 1 diabetes mellitus(FT1DM) is a subtype of T1DM, which frequently induces diabetic ketoacidosis. However, no useful marker for rapid diagnosis of FT1DM exsist. We reported that patients with FT1DM have an autoantibody against Amylaseα-2A(AMY), and developed a enzyme linked immunosorbent assay(ELISA) for detecting the antibody. Because background in the system is relatively high, so, we have improved ELISA using synthetic peptide corresponding AMY as a new antigen. When we employed peptide-7 as an antigen, we obtained good results for detecting AMY autoantibody from patients with FT1DM(sensitivity 94. 3%, specificity 93. 7% by ROC analysis), which might applicable for clinical use for rapid diagnosis of FT1DM.
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