The analysis of cell death mechanism that depended on newly isolated nucleolar GTP binding protein.

• Project/Area Number: 21658020
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Applied entomology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: TSUNEIZUMI Kazuhide 独立行政法人理化学研究所, 松本分子昆虫学研究室, 専任研究員 (40280953)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,370,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,100,000 (Direct Cost: ¥1,100,000); Fiscal Year 2009: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: nucleostemin / apoptosis / cell death / ribosomal biogenesis / GTP binding protein / Drosophila / nucleolus

Research Abstract

In this study, I made clear that an intracellular signal-transducing pathway of dNS1 to cell death was independent from p53 and there was big difference in intracellular mechanism between vertebrate and Drosophila. I succeeded in the isolation of interactive factors to dNS1 and the screening for small molecular compounds that directly modulate the activity of dNS1.

Research Products

• [Journal Article] Establishment of Sf9 transformants constitutively expressing PBAN receptor variants : application to functional evaluation (2012)
  • Author(s): J. M. Lee, J. J. Hull, T. Kawai, K. Tsuneizumi, M. Kurihara, M. Tanokura, K. Nagata, H. Nagasawa and S. Matsumoto
  • Journal Title: Frontiers in Experimental Endocrinology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Drosophila GTPase Nucleostemin 2 changes cellular distribution during larval development and GTP-binding motif is essential for the nucleoplasmic localization (2011)
  • Author(s): E. Matsuo, T. Nagamine, S. Matsumoto and K. Tsuneizumi
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 75(8) Pages: 1511-1515
  • Peer Reviewed
• [Journal Article] Hormone signaling linked to silkmoth sex pheromone biosynthesis involves Ca^<2+>/calmodulin-dependent protein kinase II(CaMKII)-mediated phosphorylation of the insect PAT family protein Bombyx mori lipid storage droplet protein-1(BmLSD1) (2011)
  • Author(s): A. Ohnishi, J. J. Hull, M. Kaji, K. Hashimoto, J. M. Lee, K. Tsuneizumi, T. Suzuki, N. Dohmae, and S. Matsumoto
  • Journal Title: The Journal of Biological Chemistry Volume: 286(27) Pages: 24101-24112
  • Peer Reviewed
• [Journal Article] Robustness of the Dpp morphogen activity gradient depends on negative feedback regulation by the inhibitory Smad, Dad (2011)
  • Author(s): Y. Ogiso, K. Tsuneizumi, N. Masuda, M. Sato and T. Tabata
  • Journal Title: Development Growth and Differentiation Volume: 53(5) Pages: 668-678
  • NAID: 10029006163
  • Peer Reviewed
• [Journal Article] Drosophila GTPase Nucleostemin 2 Changes Cellular Distribution during Larval Development and the GTP-Binding Motif Is Essential to Nucleoplasmic Localization (2011)
  • Author(s): E.Matsuo, T.Nagamine, S.Matsumoto, K.Tsuneizumi
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 8 Pages: 1511-1515
  • DOI: 10.1271/bbb.110212
  • NAID: 10029591473
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Hormone signaling linked to silkmoth sex pheromone biosynthesis involves Ca^<2+>/calmodulin-dependent protein kinase II (CaMKII)-mediated phosphorylation of the insect PAT family protein Bomhyx mori lipid storage droplet protein-1 (BmLSD1) (2011)
  • Author(s): A.Ohnishi, J.J.Hull, M.Kaji, K.Hashimoto, J.M.Lee, K.Tsuneizumi, T.Suzuki, N.Dohmae, S.Matsumoto
  • Journal Title: The Journal of Biological Chemistry Volume: 286 Issue: 27 Pages: 24101-24112
  • DOI: 10.1074/jbc.m111.250555
  • Peer Reviewed
• [Journal Article] Drosophila nucleostemin 2 proved essential for early eye development and cell survival (2010)
  • Author(s): E. Matsuo, S. Kanno, S. Matsumoto and K. Tsuneizumi
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 74(10) Pages: 2120-2123
  • NAID: 10027560856
  • Peer Reviewed
• [Journal Article] Drosophila Nucleostemin 2 Proved Essential for Early Eye Development and Cell Survival. (2010)
  • Author(s): 松尾恵倫子
  • Journal Title: Biosci.Biotechnol.Biochem. Volume: 74 Pages: 2120-2123
  • Peer Reviewed
• [Journal Article] Robustness of the Dpp morphogen activity gradient depends on negative feedback regulation by the inhibitory Smad, Dad.
  • Author(s): 小木曽由梨
  • Journal Title: Development Growth and Differentiation Volume: (in press)
  • Peer Reviewed
• [Presentation] ショウジョウバエ新規遺伝子Drosophila nucleostemin 2の機能解析 (2011)
  • Author(s): 松尾恵倫子、永峰俊弘、松本正吾、常泉和秀
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都(東山区)
  • Year and Date: 2011-03-27
• [Presentation] ショウジョウバエ新規遺伝子Drosophila nucleostemin 2の機能解析 (2011)
  • Author(s): 松尾恵倫子
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都府京都市
• [Presentation] Functional analysis of Drosophila nucleolar GTP binding protein 1(dngbp1) in response to stress (2010)
  • Author(s): K. Tsuneizumi and S. Matsumoto
  • Organizer: RIKEN Symposium on 2010 RIKEN Chemical Biology International Symposium
  • Place of Presentation: Saitama(Wako)
  • Year and Date: 2010-10-27
• [Presentation] ショウジョウバエ新規遺伝子Drosophila nucleolar GTP binding protein 2の機能解析 (2010)
  • Author(s): 松尾恵倫子、常泉和秀、松本正吾
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京(文京区)
  • Year and Date: 2010-03-30
• [Presentation] ショウジョウバエ新規遺伝子 Drosophila nucleolar GTP binding protein 2の機能解析 (2010)
  • Author(s): 松尾恵倫子
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京都目黒区
  • Year and Date: 2010-03-30
• [Presentation] Functional analysis of Drosophila nucleolar GTP binding protein 1 (dngbp1) in response to stress. (2010)
  • Author(s): 常泉和秀
  • Organizer: 2010 RIKEN Chemical Biology International Symposium
  • Place of Presentation: 埼玉県和光市