Development of oral inactivated recombinant Salmonella vaccine using E.coli cell surface display technique

• Project/Area Number: 21780290
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Clinical veterinary science
• Research Institution: Osaka Prefecture University
• Principal Investigator: TANI Hiroyuki 大阪府立大学, 生命環境科学研究科, 准教授 (00305658)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: サルモネラ / 不活化経口ワクチン / 大腸菌表面発現

Research Abstract

In order to develop oral inactivated Salmonella Enteritidisvaccine, tne E. coli surface display plasmid vector was newly constructed in this study. The N-terminal domain of MisL, which is classified as Salmonella type V secretion system, was employed an anchor protein for surface display of recombinant protein. The fusion proteins of fully functional cholera toxin B subunit (CTB), which is one of the suitable candidates for mucosal adjuvant, and Salmonella Enteritidis vaccine candidate proteins were able to be displayed on the surface of E. coli. The chickens were immunized orally with the inactivated E. coli expressed fusion proteins. The CTB-specific IgA titers in intestinal mucus were enhanced. Our findings obtained in this study can be applied to development of simple and safety oral SE vaccine.

Research Products

• [Journal Article] Phylogenetic identification of Cystoisospora spp. from dogs, cats, and raccoon dogs in Japan (2011)
  • Author(s): Matsubayashi M, Carreno RA, Tani H, Yoshiuchi R, Kanai T, Kimata I, Uni S, Furuya M,Sasai K.
  • Journal Title: Vet Parasitol Volume: 176(2-3) Pages: 270-4
  • Peer Reviewed
• [Journal Article] Effect of low pH on the morphology and viability of Cryptosporidium andersoni sporozoites and histopathology in the stomachs of infected mice. (2011)
  • Author(s): Matsubayashi M, Ando H, Kimata I, Takase H, Nakagawa H, Furuya M, Tani H, Sasai K.
  • Journal Title: Int J Parasitol Volume: 41(3-4) Pages: 287-92
  • Peer Reviewed
• [Journal Article] Survey and molecular characterization of Cryptosporidium and Giardia spp. in owned companion animal, dogs and cats, in Japan. (2010)
  • Author(s): Yoshiuchi R, Matsubayashi M, Kimata I, Furuya M, Tani H, Sasai K.
  • Journal Title: Vet Parasitol Volume: 174(3-4) Pages: 313-6
  • Peer Reviewed
• [Journal Article] Morphological changes and viability of Cryptosporidium parvum sporozoites after excystation in cell-free culture media. (2010)
  • Author(s): Matsubayashi M, Ando H, Kimata I, Nakagawa H, Furuya M, Tani H, Sasai K
  • Journal Title: Parasitology. Volume: 137(13) Pages: 1861-6
  • Peer Reviewed
• [Journal Article] Coprological survey of parasitic infections in pigs and cattle in slaughterhouse in Osaka, Japan (2009)
  • Author(s): Matsubayashi M, Kita T, Narushima T, Kimata I, Tani H, Sasai K, Baba E.
  • Journal Title: J Vet Med Sci Volume: 71(8) Pages: 1079-83
  • NAID: 130000134457
  • Peer Reviewed
• [Journal Article] Importance of subunit vaccine antigen of major Fli C antigenic site of Salmonella enteritidis II: a challenge trial. (2009)
  • Author(s): Toyota-Hanatani Y, Kyoumoto Y, Baba E, Ekawa T, Ohta H, Tani H, Sasai K.
  • Journal Title: Vaccine. Volume: 27(11) Pages: 1680-4
  • Peer Reviewed