Molecular mechanism of networking system between bone and hematopoietic cells via Wnt signaling pathway

• Project/Area Number: 22390346
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Hokkaido University
• Principal Investigator: TAMURA Masato 北海道大学, 歯学研究科(研究院), 教授 (30236757)
• Co-Investigator(Kenkyū-buntansha): NASHIMOTO Masayuki 新潟薬科大学, 応用生物学部, 教授 (30228069) ; NEMOTO Eiji 東北大学, 大学院・歯学研究科, 准教授 (40292221) ; SATO Mari 北海道大学, 大学院・歯学研究科, 助教 平成25年度 (40546488)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 骨芽細胞 / Wntシグナル / 骨細胞 / 骨髄ストローマ細胞 / CXCL12

Research Abstract

Canonical Wnt signaling molecule such as Wnt3a down-regulated CXCL12 expression in murine ST-2 cells. The culture supernatant from Wnt3a-ST2 cells also reduced migratory activity of bone marrow-derived cells and hematopoietic cell in a migration assay. Screostin which inhibits canonical Wnt signaling is identified as a signaling molecule from osteocytes to hematopoietic cell. The expression of neuropeptide Y Y1 receptor mRNA was induced by BMP2. These results raise the possibility that NPY acts in osteoblasts and osteocytes via an autocrine mechanism. From this study, functional crosstalk of regulation between bone and the other tissue.

Research Products

• [Journal Article] A naked RNA heptamer targeting the human Bc1-2 mRNA induces apoptosis of HL60 leukemia cells. (2013)
  • Author(s): Masavuki Takahashi, Reyad A. Elbarbry, Aiko Nakashima, Mayumi Abe, Norihiro Watanabe, Miwako Narita, Masuhiro Takahashi, Masato Tamura, Tetsuo Yoshida, Masayuki Nashimoto.
  • Journal Title: Cancer Letters Volume: 328 Issue: 2 Pages: 362-368
  • DOI: 10.1016/j.canlet.2012.10.016
  • Peer Reviewed
• [Journal Article] Regulation of neuropeptide Y Y1 receptor expression by bone morphogenetic protein 2 in C2C12 myoblasts (2013)
  • Author(s): Kurebayashi N, Sato M, Fujisawa T, Fukushima K, Tamura M
  • Journal Title: Biochem Biophys Res Commun Volume: 439(4) Issue: 4 Pages: 506-510
  • DOI: 10.1016/j.bbrc.2013.09.014
  • NAID: 120005324568
  • Peer Reviewed
• [Journal Article] Canonical Wnt signaling activates miR-34 expression during osteoblastic differentiation (2013)
  • Author(s): Tamura M, Uyama M, Sugiyama Y, Sato M
  • Journal Title: Mol Med Rep Volume: 8(6) Issue: 6 Pages: 1807-1811
  • DOI: 10.3892/mmr.2013.1713
  • NAID: 120005451572
  • Peer Reviewed
• [Journal Article] Bone-buried osteocytes regulate primary lymphoid organs and fat metabolism (2013)
  • Author(s): Sato M, Asada N, Kawano Y, Minagawa K, Kawano H, Wakahashi K, Sada A, Ikeda K, Matsui T, Katayama Y
  • Journal Title: Cell Metab Volume: 18 Issue: 5 Pages: 749-758
  • DOI: 10.1016/j.cmet.2013.09.014
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Acidic bone matrix proteins and their roles in calcification (2012)
  • Author(s): Fujisawa R, Tamura M
  • Journal Title: Front Biosci Volume: 17 Issue: 1 Pages: 1891-1903
  • DOI: 10.2741/4026
  • Peer Reviewed
• [Journal Article] Wnt5a signaling is a substantial constituent in bone morphogenetic protein-2-mediated osteoblastogenesis (2012)
  • Author(s): Nemoto E, Ebe Y, Kanaya S, Tsuchiya M, Nakamura T, Tamura M, Shimauchi H
  • Journal Title: Biochem Biophys Res Commun Volume: 422(4) Issue: 4 Pages: 627-632
  • DOI: 10.1016/j.bbrc.2012.05.039
  • Peer Reviewed
• [Journal Article] Regulation of CXCL12 expression by canonical Wnt signaling in bone marrow stromal cells (2011)
  • Author(s): Tamura M, Sato MM, Nashimoto M
  • Journal Title: Int J Biochem Cell Biol Volume: 43(5) Issue: 5 Pages: 760-767
  • DOI: 10.1016/j.biocel.2011.01.021
  • NAID: 120002834793
  • Peer Reviewed
• [Journal Article] Expanding the utility of heptamer-type sgRNA for TRUE gene silencing (2011)
  • Author(s): Takako Sano, Masayuki Takahashi, Tadasuke Nozaki, Yoshiaki Takahashi, Masato Tamura, Masayuki Nashimoto
  • Journal Title: Biochem Biophys Res Commun Volume: 416 Issue: 3-4 Pages: 427-432
  • DOI: 10.1016/j.bbrc.2011.11.091
  • Peer Reviewed
• [Journal Article] Regulation of CXCL12 expression by canonical Wnt signaling in bone marrow stromal cells. (2011)
  • Author(s): Tamura M
  • Journal Title: Int J Biochem Cell Biol Volume: 印刷中
  • NAID: 120002834793
• [Journal Article] 体性幹細胞の老化と若返り (2011)
  • Author(s): 佐藤真理
  • Journal Title: 北海道歯学雑誌 Volume: 印刷中
  • NAID: 10028208757
• [Journal Article] Role of the Wnt signaling pathway in bone and tooth (2010)
  • Author(s): Tamura M, Nemoto E, Sato MM, Nakashima A, Shimauchi H
  • Journal Title: Front Biosci Volume: E2 Issue: 1 Pages: 1405-1413
  • DOI: 10.2741/201
  • Peer Reviewed
• [Journal Article] 体性幹細胞の老化と若返り (2010)
  • Author(s): 佐藤真理, 田村正人
  • Journal Title: 北海道歯学雑誌 Volume: 31 Pages: 124-126
  • NAID: 10028208757
  • URL: http://eprints.lib.hokudai.ac.jp/dspace/handle/2115/45821
  • Peer Reviewed
• [Journal Article] Role of the Wnt signaling pathway in bone and tooth. (2010)
  • Author(s): Tamura M
  • Journal Title: Front Biosci. Volume: E2 Pages: 1405-1413
  • Peer Reviewed
• [Presentation] Neuropeptide Y Y1受容体のBMP2 による発現誘導と骨芽細胞における機能 (2013)
  • Author(s): 紅林奈央子, 佐藤真理, 藤澤俊明, 福島和昭, 田村正人
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場, 兵庫県神戸市
• [Presentation] Canonical Wnt signaling activates miR-34 expression during osteoblastic differentiation (2013)
  • Author(s): Tamura M, Uyama M, Kawanami M, Sato M
  • Organizer: 2nd Joint Meeting of the International Bone and Mineral Society and the Japanese Society for Bone and Mineral Research
  • Place of Presentation: Kobe Convention Center, Kobe, Japan
• [Presentation] Neuropeptide Y Y1受容体のBMP2による発現誘導と骨芽細胞における機能 (2013)
  • Author(s): 紅林奈央子
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
• [Presentation] Actions of small molecular inhibitors on anabolic effects of intermittent PTH treatment (2012)
  • Author(s): Uyama M, Kawanami M, Tamura M
  • Organizer: 2012 Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Minneapolis Convention Center, Minneapolis, MN, USA
• [Presentation] 古典的Wntシグナルは骨髄ストローマ細胞のCXCL12発現を調節する (2011)
  • Author(s): 田村正人
  • Organizer: 第29回日本骨代謝学会学術大会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2011-07-30
• [Presentation] Regulation of both osteoblastic and myogenic differentiation by nroteoasome inhibitor bortezomib (2011)
  • Author(s): Uyama M
  • Organizer: The 4th International Symposium for Interface Oral Health Science in Sendai
  • Place of Presentation: ホテル仙台プラザ(仙台市)
  • Year and Date: 2011-03-08
• [Presentation] 古典的Wntシグナルは骨髄ストローマ細胞のCXCL12発現を調節する (2011)
  • Author(s): 田村 正人, 佐藤 真理
  • Organizer: 第29回日本骨代謝学会学術大会
  • Place of Presentation: 大阪国際会議場,大阪市
• [Presentation] プロテアソーム阻害剤による骨芽細胞分化と筋細胞分化の制御 (2010)
  • Author(s): 鵜山真紀
  • Organizer: 第33回日本分子生物学会、第83回日本生化学会大会合同大会(BMB2010)
  • Place of Presentation: 神戸国際展示場(神戸市)
  • Year and Date: 2010-12-08
• [Presentation] Canonical Wnt signaling regulates CXCL12 expression of stromal osteoblasts. (2010)
  • Author(s): Tamura M
  • Organizer: 32th Annual Meeting of the American Society for Bone and Mineral Research 2010
  • Place of Presentation: Toronto Convention Center (Toronto, Canada)
  • Year and Date: 2010-10-17
• [Presentation] Canonical Wnt signaling regulates CXCL12 expression of stromal osteoblasts (2010)
  • Author(s): Tamura M, Sato M
  • Organizer: 32th Annual Meeting of the American Society for Bone and Mineral Research 2010
  • Place of Presentation: Metro Toronto Convention Centre, Toronto, ON, Canada
• [Presentation] An Anionic Dendrimer as a Model for Acidic Matrix Proteins of Bone and Tooth (2010)
  • Author(s): Fujisawa R, Mizuno M and Tamura M
  • Organizer: General Session & Exhibition of the IADR
  • Place of Presentation: Barcelona International Convention Center, Barcelona, Spain
• [Remarks]
  • URL: http://www.den.hokudai.ac.jp/seika/SeikaPub.html