Effect of valproic acid and low dose docetaxel for radiosensitivity in esophageal squamous cell carcinoma

• Project/Area Number: 22591380
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Radiation science
• Research Institution: Kanazawa University
• Principal Investigator: NINOMIYA Itasu 金沢大学, 大学病院, 准教授 (60345618)
• Co-Investigator(Kenkyū-buntansha): FUJIMURA Takashi 金沢大学, 医学系, 准教授 (50262580)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 放射線治療学 / 食道癌 / 化学放射線療法 / バルプロ酸 / DNA二重鎖切断 / ドセタキセル / HDAC阻害剤 / DNA二重鎖切断修復酵素

Research Abstract

We evaluated the Effect of valproic acid (VPA) and low dose docetaxel (DTX) for radiosensitivity in esophageal squamous cell carcinoma (ESCC). VPA induced acetylation of histones H3 and H4 in ESCC cells. VPA inhibited the DNA double strand break repair by decreasing Rad51 expression and Ku70 acetylation. VPA enhances ESCC cells radiosensitivity by chromatin decondensation and down regulation of DNA double strand break repair proteins. Low dose (5 nM) DTX induced G1 and G2-M accumulation in ESCC cell, respectively. Low dose DTX (

Research Products

• [Journal Article] Valproic acid,a histone deacetylase inhibitor,enhances radiosensitivity in esophageal squamous cell carcinoma (2012)
  • Author(s): Shoji M
  • Journal Title: Int J Oncol Volume: 40 Pages: 2140-2146
  • DOI: 10.3892/ijo.2012.1416
  • Peer Reviewed
• [Presentation] Valproic acid, a histone deacetylase inhibitor,enhances radiosensitivity in esophageal squamous cell carcinoma (2012)
  • Author(s): Ninomiya I, Shoji M, Fujimura T
  • Organizer: International Symposium on Biochemistry & Biophysics
  • Place of Presentation: Sheraton ottawa hotel (Canada)
  • Year and Date: 2012-10-25
• [Presentation] 他 食道癌細胞に対するドセタキセルの放射線感受性増強効果 (2012)
  • Author(s): 二宮 致、宮永章平、藤村 隆
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: Royton sapporo(北海道)
  • Year and Date: 2012-09-20
• [Presentation] 他バルプロ酸はヒストン脱アセチル化酵素阻害によって、食道癌細胞株の放射線感受性を増強させる (2011)
  • Author(s): 二宮 致、 正司正寿
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-11-03
• [Presentation] バルプロ酸はヒストン脱アセチル化酵素阻害によって、食道癌細胞株の放射線感受性を増強させる (2011)
  • Author(s): 二宮致
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋)
  • Year and Date: 2011-11-03
• [Presentation] 食道癌細胞に対するドセタキセルの放射線感受性増強効果
  • Author(s): 二宮 致
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: Royton sapporo(北海道）
• [Presentation] Valproic acid, a histone deacetylase inhibitor,enhances radiosensitivity in esophageal squamous cell carcinoma
  • Author(s): Ninomiya I
  • Organizer: International Symposium on Biochemistry & Biophysics
  • Place of Presentation: Sheraton Ottawa hotel（Canada）