Identification of new determinants for double fertilization
| Project/Area Number |
22657017
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphology/Structure
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| Research Institution | Chiba University (2011-2012)
The Institute of Physical and Chemical Research (2010) |
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Principal Investigator |
IGAWA Tomoko 千葉大学, 大学院・園芸学研究科, 助教 (00360488)
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| Research Collaborator |
YAMADA Lixy 名古屋大学, 大学院・理学研究科, 助教 (10551020)
MORI Toshiyuki 早稲田大学, 高等研究所, 助教 (00462739)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,580,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | 重複受精 / 配偶子細胞 / ライブイメージング / 配偶子膜 / 卵細胞膜 |
|---|
| Research Abstract |
Arabidopsis marker lines, where gamete membrane was specifically visualized with fluorescent protein (GFP), were produced. Membrane proteins were purified from pistil tissue of the egg cell membrane- marker line by pull-down strategy using anti-GFP antibody. Among the identified proteins with putative trans-membrane region, one protein was found to be involved in seed fertility.
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Report
(4 results)
Research Products
(4 results)
