| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
Streptococcal disease (streptococcosis) caused by Streptococcus iniae is a serious bacterial disease in rainbow trout (Oncorhynchus mykiss). Developing streptococcosis-resistant rainbow trout will reduce the number of outbreaks of this disease as well as reduce the need for medicines and vaccines. Genetic linkage analysis is an effective method for identifying quantitative trait loci (QTL) associated with resistance to a disease. In this study, microsatellite markers selected from genetic linkage maps of rainbow trout and BC_1 progeny from crosses between disease-resistant and disease-susceptible parents were used for detection of QTL associated with resistance to this disease. The immune-related genes on the same linkage group are correlated with significant QTL about disease resistance. Especially some studies have reported that QTLs are located on the same linkage group with the MHC class I, II or toll-like receptor regions. Linkage groups on QTL region were specifically targeted in these studies because they carry the classical MHC class I and II genes previously linked to differences in susceptibility to viral, bacterial, and parasitic diseases. Some loci associated with disease resistance were found in the RT-3、RT-16、RT-2、RT-29、RT-27、RT-31、RT-17and RT-22 linkage groups. These QTL regions are candidates for disease resistance against streptococcal infection.
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