Rapid identification and quantitation of protein by using mass spectrometry
Project/Area Number |
22790607
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Legal medicine
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Research Institution | National Research Institute of Police Science |
Principal Investigator |
KANAMORI Mieko 科学警察研究所, 法科学第三部, 主任研究官 (80356203)
|
Project Period (FY) |
2010 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 蛋白質 / プロテオーム / 分析化学 / LC/MS / MALDI-TOF/MS / 生化学 / ペプチドシーケンシング / ナノLC/MS |
Research Abstract |
Ricin and bovine casein were used as model proteins for tryptic digestion and peptide identification by liquid chromatography/mass spectrometry(LC/MS) or matrix-assisted laser desorption ionization-(time-of-flight)/MS(MALDI-TOF/MS). Marker peptides for identification of each protein were selected. In case of ricin, extraction method based on lactose affinity was developed as a method for rapid and accurate determination of ricin. Lactose was immobilized on monolithic silica, was used as a capture ligand for ricin extraction from the sample solution, and the silica was supported in a disk-packed spin column. It took approximately 5 h to carry out the entire procedure through extraction, detection and identification of ricin from crude samples.
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Report
(3 results)
Research Products
(7 results)