• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification and spatiotemporal dynamics of biofilm matrix component proteins in Staphylococcus aureus

Research Project

Project/Area Number 22880034
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionJikei University School of Medicine

Principal Investigator

SUGIMOTO Shinya  東京慈恵会医科大学, 医学部, 助教 (60464393)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsバイオフィルム / MRSA / セリンプロテアーゼ / プロテオーム解析 / 分子シャペロン / 細胞骨格タンパク質 / 細胞外マトリックス / 細菌間干渉 / 黄色ブドウ球菌 / 細胞外マトリクス / 分泌タンパク質 / 細胞壁アンカータンパク質 / アミロイド線維 / トランスポゾン / 細胞外プロテアーゼ
Research Abstract

Staphylococcus aureus exhibits a strong capacity to attach to the surface of implanted medical devices and forms multilayered communities of bacteria, called biofilm. S. aureus is a frequent cause of biofilm-associated infections that are a tremendous burden on our healthcare system. Here, we identified and characterized biofilm-matrix proteins which hold cells and contribute to bacterial accumulation in multiple layers. In addition, we found that extracellular serine protease Esp, secreted by a subset of commensal Staphylococcus epidermidis, inhibits nasal colonization of S. aureus via degradation of S. aureus surface proteins and host receptors that are crucial for biofilm formation and host-pathogen interactions. These findings not only provide novel insights into fundamental principles underlying S. aureus biofilm development, but may also indicate a novel direction for therapeutic intervention against biofilm infections.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (20 results)

All 2012 2011 2010 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (10 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Cloning, expression and purification of extracellular serine protease Esp, a biofilm-degrading enzyme, from Staphylococcus epidermidis2011

    • Author(s)
      Shinya Sugimoto, Tadayuki Iwase, Fumiya Sato, Akiko Tajima, Hitomi Shinji, and Yoshimitsu Mizunoe
    • Journal Title

      J. Appl. Microbiol

      Volume: 111 Pages: 1406-1415

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cloning, expression and purification of extracellular serine protease Esp, a biofilm-degrading enzyme, from Staphylococcus epidermidis2011

    • Author(s)
      Sugimoto S, Iwase T, Tajima A, Shinji H, Mizunoe Y
    • Journal Title

      J. Appl. Microbiol. vol.111

      Volume: 111 Issue: 6 Pages: 1406-1415

    • DOI

      10.1111/j.1365-2672.2011.05167.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of Fibronectin-Binding Proteins A and B in in vitro cellular and in vivo septic infections by Staphylococcus aureus2011

    • Author(s)
      Shinji H, Yoshizawa Y, Tajima A, Iwase T, Sugimoto S, Seki K, Mizunoe Y
    • Journal Title

      Infect. Immun

      Volume: 79 Issue: 6 Pages: 2215-2223

    • DOI

      10.1128/iai.00133-11

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Positive cooperativity of the p97 AAA ATPase is critical for essential functions.2011

    • Author(s)
      Nishikori, S., Esaki, M., Yamanaka, K., Sugimoto, S., and Ogura, T.
    • Journal Title

      J. Biol. Chem.

      Volume: 286 Issue: 18 Pages: 15815-15820

    • DOI

      10.1074/jbc.m110.201400

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improvement of multiple-stress tolerance and lactic acid production in Lactococcus lactis NZ9000 under conditions of thermal stress by heterologous expression of Escherichia coli dnaK2010

    • Author(s)
      Abdullah-Al-Mahin, Sugimoto S., Higashi C., Matsumoto S., Sonomoto K.
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 76 Pages: 4277-4285

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Staphylococcus epidermidis Esp degrades novel biofilm matrix proteins in Staphylococcus aureus biofilms2012

    • Author(s)
      Shinya Sugimoto, Takeo Iwamoto, Koji Takada, Fumiya Sato, Tadayuki Iwase, Akiko Tajima, Ippei Hironaka, Ken. ichi Okuda, Hitomi Shinji, and Yoshimitsu Mizunoe
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Cloning, expression and purification of extracellular serine protease Esp, a biofilm-degrading enzyme, from Staphylococcus epidermidis2012

    • Author(s)
      Ken. ichi Okuda, Shinya Sugimoto, Tadayuki Iwase, Fumiya Sato, Akiko Tajima, Hitomi Shinji, and Yoshimitsu Mizunoe
    • Organizer
      2012 Annual Conference of the Association for General and Applied Microbiology
    • Place of Presentation
      Tubingen, Germany
    • Related Report
      2011 Final Research Report
  • [Presentation] Cloning, expression and purification of extracellular serine protease Esp, a biofilm-degrading enzyme, from Staphylococcus epidermidis2012

    • Author(s)
      奥田賢一、杉本真也、岩瀬忠行、佐藤文哉、田嶌亜紀子、進士ひとみ、水之江義充
    • Organizer
      2012 Annual Conference of the Association for General and Applied Microbiology
    • Place of Presentation
      ドイツ(Tubingen)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Proteomic survey for Staphylococcus aureus biofilm matrix proteins : extracellular AAA+chaperone ClpB contributes to the biofilm formation2011

    • Author(s)
      Shinya Sugimoto, Takeo Iwamoto, Fumiya Sato, Akiko Tajima, Tadayuki Iwase, Hitomi Shinji, and Yoshimitsu Mizunoe
    • Organizer
      9th International Conference on AAA Proteins
    • Place of Presentation
      Kumamoto
    • Related Report
      2011 Final Research Report
  • [Presentation] 細胞外分子シャペロンによる黄色ブドウ球菌のバイオフィルム形成機構2011

    • Author(s)
      杉本真也, 岩本武夫, 佐藤文哉, 田嶌亜希子, 岩瀬忠行, 進士ひとみ, 水之江義充
    • Organizer
      日本細菌学会関東支部総会
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Efficient and economic extracellular production and purification of Staphylococcus epidermidis Esp using a Brevibacillus choshinensis secretion system2011

    • Author(s)
      Shinya Sugimoto, Tadayuki Iwase, Fumiya Sato, Akiko Tajima, Hitomi Shinji, Yukiko Yoshizawa, and Yoshimitsu Mizunoe
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo
    • Related Report
      2011 Final Research Report
  • [Presentation] Efficient and economic extracellular production and purfication of Staphylococcus epidermidis Esp using a Brevibacillus choshinensis secretion system2011

    • Author(s)
      杉本真也、岩瀬忠行、佐藤文哉、田嶌亜紀子、進士ひとみ、吉沢幸夫、水之江義充
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌
    • Related Report
      2011 Annual Research Report
  • [Presentation] Proteomic survey for Staphylococcus aureus biofilm matrix proteins : extracellular AAA+ chaperone ClpB contributes to the biofilm formation2011

    • Author(s)
      杉本真也、岩本武夫、佐藤文哉、田嶌亜紀子、岩瀬忠行、進士ひとみ、水之江義充
    • Organizer
      9th International Conference on AAA Proteins
    • Place of Presentation
      熊本
    • Related Report
      2011 Annual Research Report
  • [Presentation] バクテリア細胞内タンパク質のアミロイド様凝集体形成2011

    • Author(s)
      杉本真也、Fabian Seyffer、有田健一、荒木令江、山中邦俊、小椋光
    • Organizer
      第34回日本分子生物学会
    • Place of Presentation
      横浜
    • Related Report
      2011 Annual Research Report
  • [Presentation] Nucleotide-dependent formation of amyloid-like aggregates of Escherichia coli cytoplasmic proteins.2010

    • Author(s)
      Sugimoto, S., Araki, N., Mogk, A., Bukau, B., Ogura, T.
    • Organizer
      The 3rd International Symposium on Protein Community
    • Place of Presentation
      奈良
    • Related Report
      2010 Annual Research Report
  • [Book] Lactic Acid Bacteria and Bifidobacteria : Current Progress in Advanced Research(分担)2011

    • Author(s)
      杉本真也、園元謙二
    • Total Pages
      286
    • Publisher
      Caister Academic Press
    • Related Report
      2011 Annual Research Report
  • [Book] 乳酸菌とビフィズス菌のサイエンス2010

    • Author(s)
      杉本真也、園元謙二
    • Total Pages
      7
    • Publisher
      京都大学学術出版会
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://square.umin.ac.jp/saikin/index.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://square.umin.ac.jp/saikin/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://square.umin.ac.jp/saikin/

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-27   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi