Identification and spatiotemporal dynamics of biofilm matrix component proteins in Staphylococcus aureus
| Project/Area Number |
22880034
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | バイオフィルム / MRSA / セリンプロテアーゼ / プロテオーム解析 / 分子シャペロン / 細胞骨格タンパク質 / 細胞外マトリックス / 細菌間干渉 / 黄色ブドウ球菌 / 細胞外マトリクス / 分泌タンパク質 / 細胞壁アンカータンパク質 / アミロイド線維 / トランスポゾン / 細胞外プロテアーゼ |
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| Research Abstract |
Staphylococcus aureus exhibits a strong capacity to attach to the surface of implanted medical devices and forms multilayered communities of bacteria, called biofilm. S. aureus is a frequent cause of biofilm-associated infections that are a tremendous burden on our healthcare system. Here, we identified and characterized biofilm-matrix proteins which hold cells and contribute to bacterial accumulation in multiple layers. In addition, we found that extracellular serine protease Esp, secreted by a subset of commensal Staphylococcus epidermidis, inhibits nasal colonization of S. aureus via degradation of S. aureus surface proteins and host receptors that are crucial for biofilm formation and host-pathogen interactions. These findings not only provide novel insights into fundamental principles underlying S. aureus biofilm development, but may also indicate a novel direction for therapeutic intervention against biofilm infections.
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Report
(3 results)
Research Products
(20 results)
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[Presentation] Cloning, expression and purification of extracellular serine protease Esp, a biofilm-degrading enzyme, from Staphylococcus epidermidis2012
Author(s)
Ken. ichi Okuda, Shinya Sugimoto, Tadayuki Iwase, Fumiya Sato, Akiko Tajima, Hitomi Shinji, and Yoshimitsu Mizunoe
Organizer
2012 Annual Conference of the Association for General and Applied Microbiology
Place of Presentation
Tubingen, Germany
Related Report
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