Analysis of p53 72SNP in stress responce

• Project/Area Number: 23501267
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: Kyoto University
• Principal Investigator: HABU Toshiyuki 京都大学, 放射線生物研究センター, 助教 (70346071)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: p53 / SNP / HPV / がん抑制遺伝子 / 遺伝子改変 / 外的要因ストレス / パピローマウイルス

Research Abstract

Single nucleotide polymorphism(SNP) affect disease and stress responses. To address the relationship between SNP and stress response, p53 72SNP was introduced in human cell line using genetic recombination method. These cells showed the different responses under DNA damage agents although these cells have same genetic background. These results indicated that p53 SNP affect physiological output and these approach are useful for SNP study.

Research Products

• [Journal Article] Pcf1, a large subunit of CAF-1, required for maintenance of checkpoint kinase Cds1 activity (2014)
  • Author(s): Kunoh T and Habu T
  • Journal Title: Springer Plus Volume: 3 Pages: 30-30
• [Journal Article] 1. Pcf1, a large subunit of CAF-1, required for maintenance of checkpoint kinase Cds1 activity (2014)
  • Author(s): Kunoh T and Habu T.
  • Journal Title: Springer Plus Volume: 3:30 Issue: 1 Pages: 1-8
  • DOI: 10.1186/2193-1801-3-30
  • Peer Reviewed
• [Journal Article] The p31^<comet> inactivates the chemically induced Mad2-dependent spindle assembly checkpoint and leads to resistance to anti-mitotic drugs (2013)
  • Author(s): Habu T and Matsumoto T
  • Journal Title: Springer Plus Volume: 2 Pages: 562-562
• [Journal Article] The moyamoya disease susceptibility variant RNF213 1 R4810K induces genomic instability by mitotic abnormality (2013)
  • Author(s): Hitomi T, Habu T, Kobayashi H, Okuda H, Harada HK, Osafune K, Taura D, Sone M, Asaka I, Ameku T, Watanabe A, Kasahara T, Sudo T, Shiota F, Hashikata H, Takagi Y, Morito D, Miyamoto S, Nakao K, Koizumi A
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 439(4) Pages: 419-426
• [Journal Article] Downregulation of Securin by the variant RNF213 R4810K (rs112735431, G>A) reduces angiogenic activity of induced pluripotent stem cell-derived vascular endothelial cells from moyamoya patients (2013)
  • Author(s): Hitomi T, Habu T, Kobayashi H, Okuda H, Harada KH, Osafune K, Taura D, Sone M, Asaka I, Ameku T, Watanabe A, Kasahara T, Sudo T, Shiota F, Hashikata H, Takagi Y, Morito D, Miyamoto S, Nakao K, Koizumi A
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 438(1) Pages: 13-19
• [Journal Article] An E2 enzyme Ubc11 is required for ubiquitination of Slp1/Cdc20 and spindle checkpoint silencing in fission yeast (2013)
  • Author(s): Horikoshi Y, Habu T, Matsumoto T
  • Journal Title: Cell Cycle Volume: 12(6) Pages: 961-971
• [Journal Article] p31comet inactivates the chemically induced Mad2-dependent spindle assembly checkpoint and leads to resistance to anti-mitotic drugs (2013)
  • Author(s): Habu T and Matsumoto T.
  • Journal Title: Springer Plus Volume: 2:562 Issue: 1 Pages: 1-15
  • DOI: 10.1186/2193-1801-2-562
  • Peer Reviewed
• [Journal Article] The moyamoya disease susceptibility variant RNF213 R4810K (rs112735431) (2013)
  • Author(s): Hitomi T, Habu T, Kobayashi H, Okuda H, Harada KH, Osafune K, Taura D, Sone M, Asaka I, Ameku T, Watanabe A, Kasahara T, Sudo T, Shiota F, Hashikata H, Takagi Y, Morito D, Miyamoto S, Nakao K, Koizumi A
  • Journal Title: Biochem Biophys Res Commun. Volume: 439(4) Issue: 4 Pages: 419-426
  • DOI: 10.1016/j.bbrc.2013.08.067
  • Peer Reviewed
• [Journal Article] Downregulation of Securin by the variant RNF213 R4810K (rs112735431, G>A) reduces angiogenic activity of induced pluripotent stem cell-derived vascular endothelial cells from moyamoya patients. (2013)
  • Author(s): Hitomi T, Habu T, Kobayashi H, Okuda H, Harada KH, Osafune K, Taura D, Sone M, Asaka I, Ameku T, Watanabe A, Kasahara T, Sudo T, Shiota F, Hashikata H, Takagi Y, Morito D, Miyamoto S, Nakao K, Koizumi A.
  • Journal Title: Biochem Biophys Res Commun. Volume: 438(1): Issue: 1 Pages: 13-19
  • DOI: 10.1016/j.bbrc.2013.07.004
  • Peer Reviewed
• [Journal Article] An E2 enzyme Ubc11 is required for ubiquitination of Slp1/Cdc20 and spindle checkpoint silencing in fission yeast. (2013)
  • Author(s): Horikoshi Y, Habu T, Matsumoto T.
  • Journal Title: Cell Cycle Volume: 12:6 Issue: 6 Pages: 961-971
  • DOI: 10.4161/cc.23946
  • Peer Reviewed
• [Journal Article] Ablation of Rnf213 retards progression of diabetes in the Akita mouse (2013)
  • Author(s): Kobayashi H, Yamazaki S, Takashima S, Liu W, Okuda H, Yan J, Fujii Y, Hitomi T, Harada KH, Habu T, Koizumi A
  • Journal Title: Biochem Biophys Res Commun Volume: 432 Issue: 3 Pages: 519-525
  • DOI: 10.1016/j.bbrc.2013.02.015
  • Peer Reviewed
• [Journal Article] A mutation of the fission yeast EB1 overcomes negative regulation by phosphorylation and stabilizes microtubules (2012)
  • Author(s): Iimori M, Ozaki K, Chikashige Y, Habu T, Hiraoka Y, Maki T, Hayashi I, Obuse C, Matsumoto T
  • Journal Title: Exp. Cell Res Volume: 318(3) Pages: 262-275
  • NAID: 120003874262
• [Journal Article] A mutation of the fission yeast EB1 overcomes negative regulation by phosphorylation and stabilizes microtubules (2012)
  • Author(s): Iimori, M.
  • Journal Title: Exp Cell Res Volume: 318 Issue: 3 Pages: 262-275
  • DOI: 10.1016/j.yexcr.2011.11.006
  • Peer Reviewed
• [Journal Article] Genistein, abundant isoflavonoids in soybeans, prevents the formation of excess radiation-induced centrosomes via p21 up-regulation (2011)
  • Author(s): Shimada M, Kato A, Habu T and Komatsu K
  • Journal Title: Mutat Res Volume: 716(1-2) Pages: 27-32
• [Journal Article] Expansion of Intronic GGCCTG Hexanucleotide Repeat in NOP56 Causes a Type of Spinocerebellar Ataxia (SCA36) Accompanied by Motor Neuron Involvement (2011)
  • Author(s): Kobayashi H, Abe K, Matsuura T, Ikeda Y, Hitomi T, Akechi Y, Habu T, Yang WL, Okuda H, and Koizumi A
  • Journal Title: Am. J. Hum. Genet Volume: 89(1) Pages: 121-130
• [Journal Article] Expansion of Intronic GGCCTG Hexanucleotide Repeat in NOP56 Causes a Type of Spinocerebellar Ataxia (SCA36) Accompanied by Motor Neuron Involvement (2011)
  • Author(s): Hatasu Kobayashi, Koji Abe, Tohru Matsuura, Yoshio Ikeda, Toshiaki Hitomi, Yuji Akechi, Toshiyuki Habu, Wangyang Liu, Hiroko Okuda, Akio Koizumi
  • Journal Title: Am J Hum Genet Volume: 89(1) Issue: 1 Pages: 121-130
  • DOI: 10.1016/j.ajhg.2011.05.015
  • Peer Reviewed
• [Journal Article] Genistein, isoflavonoids in soybeans, prevents the formation of excess radiation-induced centrosomes via p21up-regulation (2011)
  • Author(s): Shimada M, Kato A, Habu T, Komatsu K
  • Journal Title: Mutatation Research Volume: Volume 716 Issue: 1-2 Pages: 27-32
  • DOI: 10.1016/j.mrfmmm.2011.07.017
  • Peer Reviewed
• [Presentation] Mysterin 遺伝子のR4810K 多型はもやもや病特異的iPS 細胞由来血管内皮細胞においてSecurin の発現抑制を介して血管形成能を低下させる (2014)
  • Author(s): 小林果、人見敏明、土生敏行、原田浩二、小泉昭夫
  • Organizer: 第13回 分子予防環境医学研究会
  • Place of Presentation: 和歌山県民文化会館
• [Presentation] もやもや病感受性変異体Mysterin R4810K は有糸分裂異常によりゲノム不安定性を誘導する (2014)
  • Author(s): 人見敏明、小林果、土生敏行、原田浩二、小泉昭夫
  • Organizer: 第13回 分子予防環境医学研究会
  • Place of Presentation: 和歌山県民文化会館
• [Presentation] Mysterin遺伝子のR4810K多型はもやもや病特異的iPS細胞由来血管内皮細胞においてSecurinの発現抑制を介して血管形成能を低下させる (2014)
  • Author(s): 小林果、人見敏明、土生敏行、原田浩二、小泉昭夫
  • Organizer: 第13回 分子予防環境医学研究会
  • Place of Presentation: 和歌山県民文化会館
• [Presentation] もやもや病感受性変異体Mysterin R4810Kは有糸分裂異常によりゲノム不安定性を誘導する (2014)
  • Author(s): 人見敏明、小林果、土生敏行、原田浩二、小泉昭夫
  • Organizer: 第13回 分子予防環境医学研究会
  • Place of Presentation: 和歌山県民文化会館
• [Presentation] NOP56 遺伝子イントロンにおける6塩基リピート拡張は脊髄小脳変性症36型を引き起こす (2012)
  • Author(s): 小林果, 阿部康二, 松浦徹, 池田佳生,人見敏明, 土生敏行, 劉万洋, 奥田裕子, 原田浩二, 小泉昭夫
  • Organizer: 第82回日本衛生学会学術総会
  • Place of Presentation: 京都大学吉田キャンパス
• [Presentation] ALS 様運動神経障害を伴う新規脊髄小脳変性症(SCA36)の原因遺伝子発見 (2012)
  • Author(s): 小林果、阿部康二、松浦徹、池田佳生、人見敏明、明地雄司、土生敏行、劉万洋、奥田裕子、小泉昭夫
  • Organizer: 第11回 分子予防環境医学研究会
  • Place of Presentation: 川崎医科大学
• [Presentation] Stress response controlled by differential binding of p31comet to p53 (2012)
  • Author(s): Toshiyuki Habu, Tsuyoshi Ikura, Tomohiro Matsumoto
  • Organizer: The Sugahara menorial international symposium "Prospective Topics and charm of Radiation Biology"
  • Place of Presentation: 京都大学
• [Presentation] p53codon72 アリルpolymorphism とp53-p31comet 複合体関連解析 (2012)
  • Author(s): 土生 敏行
  • Organizer: 第16回 日本がん分子標的治療学会
  • Place of Presentation: 北九州 (ポスター発表)
• [Presentation] p53codon72アリルpolymorphismとp53-p31comet複合体関連解析 (2012)
  • Author(s): 土生敏行
  • Organizer: 日本がん分子標的治療学会 第１６回学術集会
  • Place of Presentation: 北九州市
• [Presentation] ストレス誘導型p53-p31comet 複合体形成調節機構の解析 (2011)
  • Author(s): 土生 敏行
  • Organizer: 第15回 日本がん分子標的治療学会
  • Place of Presentation: 東京
• [Presentation] ストレス誘導型p53-p31comet 複合体形成調節機構の解析 (2011)
  • Author(s): 土生 敏行
  • Organizer: 第70回 日本癌学会学術総会
  • Place of Presentation: 名古屋 (ポスター発表)
• [Presentation] ストレス誘導型p53-p31comet複合体形成調節機構の解析 (2011)
  • Author(s): 土生 敏行
  • Organizer: 第15回 日本がん分子標的治療学会
  • Place of Presentation: 東京 ホテル日航東京
• [Presentation] p53-p31comet複合体による細胞死制御機構の解析 (2011)
  • Author(s): 土生 敏行
  • Organizer: 第54回大会 日本癌学会
  • Place of Presentation: 名古屋 名古屋国際会議場
• [Presentation] Moyamoya meets mitosis?
  • Author(s): 土生敏行
  • Organizer: 生命科学シンポジウム
  • Place of Presentation: 京都大学しらん会館