Identification of HOX gene which suppresses hepatic metastasis of colon cancer

• Project/Area Number: 23590451
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Hokkaido University
• Principal Investigator: HAMADA Jun-ichi 北海道大学, 遺伝子病制御研究所, 准教授 (50192703)
• Co-Investigator(Renkei-kenkyūsha): IIZASA Hisashi 北海道大学, 遺伝子病制御研究所, 助教 (80306662)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 転移 / HOX / 浸潤 / 大腸癌 / 肝転移 / ホメオボックス遺伝子 / がん転移 / 大腸がん / 位置情報

Research Abstract

In this study, we examined the roles of HOXD8 in three steps of hepatic metastasis of human colon cancer to demonstrate anti-metastatic function of HOXD8. We found the following: the cells highly invasive of subendothelial basement membrane showed higher expressions of HOXD8 than those weakly invasive; there was no correlation between adhesiveness of the cells to hepatic sinusoidal endothelial cells and the expression levels of HOXD8; the cells growing in the liver of nude mice expressed HOXD8 at higher levels than those growing in the cecum. These results suggested that HOXD8 functioned as a metastasis-enhancing or –suppressive gene depending on the step where the cells were during hepatic metastasis.

Research Products

• [Journal Article] Hypoxia affects in vitro growth of newly established cell lines from patients with malignant pleural mesothelioma (2013)
  • Author(s): Goudarzi1, H., Hida, Y., Takano, H., Teramae, H., Iizasa, H. and Hamada, J.
  • Journal Title: Biomedical Research Volume: 34 Issue: 1 Pages: 13-21
  • DOI: 10.2220/biomedres.34.13
  • NAID: 130004470830
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed
• [Journal Article] Isolation and characterization of human breast cancer cells with SOX2 promoter activity. (2013)
  • Author(s): Liang S, Furuhashi M, Nakane R, Nakazawa S, Goudarzi H, Hamada J, Iizasa H.
  • Journal Title: Biochem Biophys Res Commun Volume: 437 Issue: 2 Pages: 205-211
  • DOI: 10.1016/j.bbrc.2013.06.038
  • NAID: 120005324564
  • Peer Reviewed
• [Journal Article] Enhancement of in vitro cell motility and invasiveness of human malignant pleural mesothelioma cells through the HIF-1α-MUC1 pathway. (2013)
  • Author(s): Goudarzi H, Iizasa H, Furuhashi M, Nakazawa S, Nakane R, Liang S, Hida Y, Yanagihara K, Kubo T, Nakagawa K, Kobayashi M, Irimura T, Hamada J.
  • Journal Title: Cancer Lett Volume: 339 Issue: 1 Pages: 82-91
  • DOI: 10.1016/j.canlet.2013.07.020
  • Peer Reviewed
• [Journal Article] Mutant p53 gain-of-function induces epithelial-mesenchymal transition through modulation of the miR-130b-ZEB1 axis (2013)
  • Author(s): Dong, P., Karaayvaz, M., Jia, N., Kaneuchi,M., Hamada, J., Watari, H., Sudo, S., Ju, J. and Sakuragi, N.
  • Journal Title: Oncogene Volume: 印刷集 Issue: 27 Pages: 3286-3295
  • DOI: 10.1038/onc.2012.334
  • NAID: 10031131106
  • Peer Reviewed
• [Journal Article] Differential expressions of matrix metalloproteinases, a disintegrin and metalloproteinases, and a disintegrin and metalloproteinases with thrombospondin motifs and their endogenous inhibitors among histologic subtypes of lung cancers (2012)
  • Author(s): Hida, Y. and Hamada, J.
  • Journal Title: Anticancer Agents Med Chem. Volume: 12 Pages: 744-752
  • Peer Reviewed
• [Journal Article] Differential expressions of matrix metalloproteinases, a disintegrin and metalloproteinases, and a disintegrin and metalloproteinases with thrombospondin motifs and their endogenous inhibitors among histologic subtypes of lung cancers (2012)
  • Author(s): Hida, Y. and Hamada, J.
  • Journal Title: Anticancer Agents Med. Chem. Volume: 12 Issue: 7 Pages: 744-752
  • DOI: 10.2174/187152012802650156
  • Peer Reviewed
• [Journal Article] Differential expressions of matrix metalloproteinases, a disintegrin and metalloproteinases, and a disintegrin and metalloproteinases with thrombospondin motifs and their endogenous inhibitors among histologic subtypes of lung cancers (2012)
  • Author(s): Hida, Y. and Hamada, J.
  • Journal Title: Anticancer Agents Med Chem Volume: 印刷中
  • Peer Reviewed
• [Presentation] ホメオボックス遺伝子HOXD8のヒト大腸癌の肝転移における役割について (2013)
  • Author(s): 古橋昌子・浜田淳一
  • Organizer: 第3回IGMリサーチワークショップ
  • Place of Presentation: 札幌
  • Year and Date: 2013-03-12
• [Presentation] ホメオボックス遺伝子HOXD8のヒト大腸癌の肝転移における役割について (2013)
  • Author(s): 古橋昌子・浜田淳一
  • Organizer: 第3回IGMリサーチワークショップ
  • Place of Presentation: 北海道大学医学部フラテホール（札幌）
• [Presentation] ヒト悪性胸膜中皮腫細胞の低酸素培養状態における細胞運動・浸潤能の増強 (2012)
  • Author(s): ゴウダルジホウマヌ・飯笹久・古橋昌子・中澤誠多朗・中根梨恵・柳原五吉・久保貴紀・浜田淳一
  • Organizer: 第21回日本がん転移学会学術集会
  • Place of Presentation: オリエンタルホテル広島（広島県）
• [Presentation] Enhacement of cell motility and invasion of malignant pleural mesothelioma cells through HIF1alpha-MUC1 pathway (2012)
  • Author(s): Houman Goudarzi, Hisashi Iizasa, Masako Furuhashi, Rie Nakane, Seitaro Nakazawa, Yasuhiro Hida, Kazuyoshi Yanagihara, Takanori Kubo and Jun-ichi Hamada
  • Organizer: 71st Annual meeting of the Japanese Cancer Association
  • Place of Presentation: ロイトン札幌（札幌）
• [Presentation] Regulatory mechanisms of SOX2 expression in human breast carcinoma cells (2012)
  • Author(s): Shanshan Liang, Hisashi Iizasa, Masako Furuhashi, Seitaro Nakazawa, Rie Nakane and Jun-ichi Hamada
  • Organizer: 71st Annual meeting of the Japanese Cancer Association
  • Place of Presentation: ロイトン札幌（札幌）
• [Book] Oral Cancer InTech (2012)
  • Author(s): Hassan NMM, Tada M, Hamada J, Shindoh M, Kashiwazaki H, Yamazaki Y, Ashikaga Y, Moriuchi T, Inoue N and Sasaki A.
  • Total Pages: 22
  • Publisher: p53 Mutation and Multiple Primary Oral Squamous Cell Carcinomas
• [Remarks]
  • URL: http://www.igm.hokudai.ac.jp/crg/