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Study of bacterial adhering mechanism onto mammalian epithelial cells

Research Project

Project/Area Number 23659225
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Bacteriology (including Mycology)
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

IKE Fumio  独立行政法人理化学研究所, 独立行政法人理化学研究所, 専任研究員 (40183157)

Co-Investigator(Kenkyū-buntansha) KOKUBO Toshiaki  独立行政法人放射線医学総合研究所, 研究基盤センター, 課長 (10425663)
Research Collaborator 松下 悟  独立行政法人放射線医学総合研究所, 研究基盤センター
HATTORI Masahira  東京大学大学院新領域創成科学研究科, 大学院・新領域創成科学研究科・附属オーミクス情報センター, 教授
OSHIMA Kenshiro  東京大学, 大学院新領域創成科学研究科・附属オーミクス情報センター, 特任助教
MURATA Takehide  独立行政法人理化学研究所, 遺伝子材料開発室, 遺伝子材料開発室
OKUBO Masato  独立行政法人理化学研究所, 遺伝子材料開発室, テクニカルスタッフ
KAJITA Ayako  独立行政法人理化学研究所, 実験動物開発室, 人材派遣
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsCARバチルス / 肺炎病原菌 / 間質性肺炎 / マウス / ゲノムシークエンシング / 培養 / 感染 / 細胞付着機構
Research Abstract

The cilia-associated respiratory bacillus (CARB), a gram-negative extracellular bacterium, causes chronic respiratory disease in rodents. In this research, we got full genome sequence of CARB (about 1.44 Mbp long with around 1,200 coding sequences). Using this information, we also designed custom DNA array chip to analyze CARB's RNA expression when CARB attached to and grown on epithelial cells in vitro.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (39 results)

All 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 4 results) Presentation (30 results) (of which Invited: 1 results) Remarks (2 results)

  • [Journal Article] Reverse Transcription-Loop-Mediated Isothermal Amplification for the De-tection of Rodent Coronaviruses2013

    • Author(s)
      Hanaki K*, Ike F, Hatakeyama R, Hirano N.
    • Journal Title

      J. Virol. Meth

      Volume: 187 (2) Pages: 222-227

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reverse transcription-loop- mediated isothermal amplification for the detection of rodent coronaviruses2013

    • Author(s)
      Hanaki K, Ike F, Hatakeyama R, Hirano N.
    • Journal Title

      J Virol Methods

      Volume: 187 Issue: 2 Pages: 222-227

    • DOI

      10.1016/j.jviromet.2012.10.008

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An On-Site Serology Monitoring System Consisting of a Multiplex Microfluidic Chip Fabricated Using the Electrospray Deposition Method for Laboratory Mice2012

    • Author(s)
      Aoki H, Kaneko A, Kajita A, Yamagata Y, Ike F*, Kase H
    • Journal Title

      J. Chem. Eng. Jap.

      Volume: 45 (7) Pages: 528-538

    • NAID

      10030928742

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 齧歯類のニューモシスチス感染症2012

    • Author(s)
      池 郁生
    • Journal Title

      実験動物ニュース

      Volume: 61(4) Pages: 47-50

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] An On-Site Serology Monitoring System Consisting of a Multiplex Microfluidic Chip Fabricated Using the Electrospray Deposition Method for Laboratory Mice2012

    • Author(s)
      Aoki H, Kaneko A, Kajita A, Yamagata Y, Ike F, Kase H.
    • Journal Title

      JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

      Volume: 45 Issue: 7 Pages: 528-538

    • DOI

      10.1252/jcej.12we017

    • NAID

      10030928742

    • ISSN
      0021-9592, 1881-1299
    • Related Report
      2012 Annual Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] リンパ球性脈絡髄膜炎ウイルス感染マウスのクリーニングは可能か?2011

    • Author(s)
      池 郁生
    • Journal Title

      日本実験動物技術者協会九州支部会報

      Volume: No.35 Pages: 11-16

    • Related Report
      2011 Research-status Report
  • [Journal Article] 放射線影響を再び考える-動物実験から分かること、放射線規制のいま-2011

    • Author(s)
      小久保年章
    • Journal Title

      アニテックス

      Volume: 23巻5号 Pages: 3-3

    • Related Report
      2011 Research-status Report
  • [Presentation] Culture Method Improvement of CAR Bacillus, Rodent Pneumonic Extracel-lular Bacteria, by Adding Methyl-cellulose2013

    • Author(s)
      Ike F
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張メッセ国際会議場, 千葉県千葉市
    • Related Report
      2012 Final Research Report
  • [Presentation] What is Rodent Pneumonic CAR bacillus? - From Full Genome Sequencing2013

    • Author(s)
      Ike F. Kajita A, Yoshiki A, Okubo M, Murata T, Oshima K, Hattori M and Kokubo T
    • Organizer
      12th FELASA SECAL Congress
    • Place of Presentation
      Barcelona's International Convention Center, Barcelona, Spain
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Culture Method Improvement of CAR Bacillus, Rodent Pneumonic Extracellular Bacteria, by Adding Methylcellulose2013

    • Author(s)
      Ike F
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張メッセ国際会議場, 千葉県千葉市
    • Related Report
      2012 Annual Research Report
  • [Presentation] CARバチルスのゲノムシークエンス解析2013

    • Author(s)
      池 郁生, 梶田亜矢子, 吉木 淳, 大久保将人, 村田武英, 大島健志朗, 服部正平, 小久保年章
    • Organizer
      第60回日本実験動物学会総会
    • Place of Presentation
      つくば国際会議場, 茨城県つくば市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Emerging microbiological contamina-tion and its detection techniques in mice; Information from the quarantine at a mouse bank2012

    • Author(s)
      Ike F
    • Organizer
      2012 KALAS International Symposium
    • Place of Presentation
      Lote Buyeo Resort, Buyeo, Korea(招待講演)
    • Related Report
      2012 Final Research Report
  • [Presentation] Draft sequencing of rodent pneumonic CAR bacillus2012

    • Author(s)
      Ike F, Kajita A, Yoshiki A, Okubo M, Murata T, Oshima K, Hattori M, Kokubo T
    • Organizer
      The 11th Awaji Interna-tional Forum on Infection and Immunity
    • Place of Presentation
      Awaji Yumebutai International Con-ference Center, Awaji, Hyogo, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] CAR Bacillus Single Culture2012

    • Author(s)
      Ike F, Kajita A, Yoshiki A, Kokubo T
    • Organizer
      5th AFLAS Congress
    • Place of Presentation
      Bangkok International Trade & Exhibition Centre, Bangkok, Thailand
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Microbiological Status of Japanese Mouse Colony: From RIKEN BRC monitoring results2012

    • Author(s)
      Ike F and Yoshiki A
    • Organizer
      5th AFLAS Congress
    • Place of Presentation
      Bangkok International Trade & Exhibition Centre, Bangkok, Thailand
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Biofilm Development by Rodent Pneu-monic Extracellular Bacteria2012

    • Author(s)
      Ike F and Kokubo T
    • Organizer
      Biofilms 5
    • Place of Presentation
      Espace Saint-Martin, Paris, France
    • Related Report
      2012 Final Research Report
  • [Presentation] CARバチルス検査系の確立2012

    • Author(s)
      池 郁生, 梶田亜矢子, 吉木 淳, 松下 悟, 小久保年章
    • Organizer
      第59回日本実験動物学会総会
    • Place of Presentation
      別府国際コンベンションセンター, 大分県別府市
    • Related Report
      2012 Annual Research Report
  • [Presentation] CARバチルスがどうやってバイオフィルムを形成するかの考察2012

    • Author(s)
      池 郁生, 小久保年章
    • Organizer
      第6回細菌学若手コロッセウム
    • Place of Presentation
      八王子セミナーハウス, 東京都八王子市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Emerging microbiological contamination and its detection techniques in mice; Information from the quarantine at a mouse bank2012

    • Author(s)
      Ike F
    • Organizer
      2012 KALAS International Symposium
    • Place of Presentation
      Lotte Buyeo Resort, Buyeo, Korea
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Draft sequencing of rodent pneumonic CAR bacillus2012

    • Author(s)
      Ike F, Kajita A, Yoshiki A, Okubo M, Murata T, Oshima K, Hattori M, Kokubo T
    • Organizer
      The 11th Awaji International Forum on Infection and Immunity
    • Place of Presentation
      Awaji Yumebutai International Conference Center, Awaji, Hyogo, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] CARバチルスはなぜ孵化鶏卵で増殖するのか(1)2012

    • Author(s)
      池 郁生, 小久保年章
    • Organizer
      第154回日本獣医学会学術集会
    • Place of Presentation
      岩手大学, 岩手県盛岡市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Biofilm Development by Rodent Pneumonic Extracellular Bacteria2012

    • Author(s)
      Ike F and Kokubo T
    • Organizer
      Biofilms 5
    • Place of Presentation
      Espace Saint-Martin, Paris, France
    • Related Report
      2012 Annual Research Report
  • [Presentation] 放射線誘発腎がんの被ばく時年齢依存性2012

    • Author(s)
      小久保年章, 柿沼志津子, 樋野興夫, 島田義也
    • Organizer
      平成23年度「個体レベルでのがん研究支援活動」ワークショップ 個体レベルのがん研究のパラダイム-動物発がんモデルと分子機構の解明
    • Place of Presentation
      琵琶湖ホテル(滋賀県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 微生物コントロールに関する最近の動きについて2012

    • Author(s)
      池 郁生
    • Organizer
      第153回日本獣医学会学術集会(招待講演)
    • Place of Presentation
      大宮ソニックホール(埼玉県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Host-derived factor induces morpho-logical change in pneumonic extra-cellular bacteria2011

    • Author(s)
      Ike F, Matsushita S, Kokubo T
    • Organizer
      IUMS 2011, XII International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      Sapporo, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Four-stage Model of CAR Bacillus infection In Vitro2011

    • Author(s)
      Ike F, Kokubo T, Matsushita S, Yoshiki A.
    • Organizer
      62nd AALAS National Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] LCMV感染マウスにおける臓器中のウイルス量の経時変化2011

    • Author(s)
      高木利一, 大沢牧子, 池 郁生, 山中仁木, 佐藤 浩, 大沢一貴
    • Organizer
      第58回日本実験動物学会総会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] CARバチルスの培養細胞感染系2011

    • Author(s)
      池 郁生, 梶田亜矢子, 吉木 淳, 松下 悟, 小久保年章
    • Organizer
      第58回日本実験動物学会総会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] LAMP法による黄色ブドウ球菌の薬剤耐性、消毒薬抵抗性の判別2011

    • Author(s)
      花木賢一, 池 郁生
    • Organizer
      第58回日本実験動物学会総会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] マウスやラットに肺炎を起こす未分類菌CARバチルス2011

    • Author(s)
      池 郁生, 小久保年章
    • Organizer
      第5回細菌学若手コロッセウム
    • Place of Presentation
      国民宿舎桂浜荘(高知県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Host-derived factor induces morphological change in pneumonic extracellular bacteria2011

    • Author(s)
      Ike F, Matsushita S, Kokubo T
    • Organizer
      IUMS 2011, XII International Congress of Bacteriology and Applied Microbiology
    • Place of Presentation
      Sapporo Convention Center (Sapporo, Japan)
    • Related Report
      2011 Research-status Report
  • [Presentation] CARバチルスの単独培養2011

    • Author(s)
      池 郁生, 吉木 淳, 松下 悟, 小久保年章
    • Organizer
      第152回日本獣医学会学術集会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス(大阪府)
    • Related Report
      2011 Research-status Report
  • [Presentation] RT-LAMP法によるマウス肝炎ウイルスの検出2011

    • Author(s)
      花木賢一, 佐藤綾子, 池 郁生, 平野紀夫
    • Organizer
      第152回日本獣医学会学術集会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス(大阪府)
    • Related Report
      2011 Research-status Report
  • [Presentation] マウスにおける自然発症肺がんの組織学的検討2011

    • Author(s)
      金井孝夫, 池 郁生
    • Organizer
      第152回日本獣医学会学術集会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス(大阪府)
    • Related Report
      2011 Research-status Report
  • [Presentation] Four-stage Model of CAR Bacillus infection In Vitro2011

    • Author(s)
      Ike F, Kokubo T, Matsushita S, Yoshiki A
    • Organizer
      62nd AALAS National Meeting
    • Place of Presentation
      San Diego Convention Center (San Diego, USA)
    • Related Report
      2011 Research-status Report
  • [Presentation] 放射線が生体に与える影響について-動物実験から分かること-2011

    • Author(s)
      小久保年章
    • Organizer
      第83回実験動物コンファレンス(招待講演)
    • Place of Presentation
      日本獣医生命科学大学(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 肺癌モデルの可能性:純系マウスの自然発症例2011

    • Author(s)
      金井孝夫, 池 郁生
    • Organizer
      第83回実験動物コンファレンス
    • Place of Presentation
      日本獣医生命科学大学(東京都)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.brc.riken.jp/lab/animal/

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.brc.riken.jp/lab/animal/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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