Basic research for chronotherapy focused on liver regeneration andliver steatosis by circadian clock

• Project/Area Number: 23659398
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Yamaguchi University
• Principal Investigator: TERAI Shuji 山口大学, 大学院・医学系研究科, 准教授 (00332809)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Naoki 山口大学, 大学教育機構, 講師 (90448283) ; TAKAMI Taro 山口大学, 大学院・医学系研究科, 講師 (60511251) ; FUJISAWA Koichi 山口大学, 大学院・医学系研究科, 助教 (00448284) ; SAKAIDA Isao 山口大学, 大学院・医学系研究科, 教授 (80263763)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: NASH / 肝障害 / サーカディアンリズム / 脂肪肝 / 肝再生 / サーカデイアンリズム

Research Abstract

Medaka with HFD showed high NASH activity score (NAS), suggesting that circadian rhythm change is related to NASH progression. The light system is closely related with chronic inflammation by light stimulation. Collaboration with Oklahoma university, we found that NASH medaka developed diabetic nephropathy. These data showedmedaka fish model is useful to analyze the mechanism of NASH and nephropathyand to develop a new chronotherapy.

Research Products

• [Journal Article] Medaka fish, Oryzias latipes, as a model for human obesity-related glomerulopathy. (2013)
  • Author(s): Ichimura K, Kawashima Y, Nakamura T, Powell R, Hidoh Y, Terai S, Sakaida I, Kodera Y, Tsuji T, Ma JX, Sakai T, Matsumoto H, Obara T
  • Journal Title: Biochem Biophys Res Commun Volume: 22;431(4) Pages: 712-7
  • Peer Reviewed
• [Journal Article] Medaka fish, Oryzias latipes, as a model for human obesity-related glomerulopathy (2013)
  • Author(s): Ichimura K, Kawashima Y, Nakamura T, Powell R, Hidoh Y, Terai S, Sakaida l, Kodera Y, T suji T, Ma JX, Sakai T, Matsumoto H, Obara T
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 431 Issue: 4 Pages: 712-717
  • DOI: 10.1016/j.bbrc.2013.01.053
  • Peer Reviewed
• [Journal Article] Ezetimibe reduces fatty acid quantity in liver and decreased inflammatory cell infiltration and improved NASH in medaka model (2012)
  • Author(s): Oishi T, Terai S, Kuwashiro S, Fujisawa K, Matsumoto T, Nishina H, Sakaida I
  • Journal Title: Biochem Biophys Res Commun Volume: 25;422(1) Pages: 22-7
  • Peer Reviewed
• [Journal Article] Ezetimibe reduces fatty acid quantity in liver and decreased inflammatory cell infiltration and improved NASH in medaka model. (2012)
  • Author(s): Oishi Toshiyuki
  • Journal Title: Biochem Biophys Res Commun. Volume: 422(1) Issue: 1 Pages: 22-27
  • DOI: 10.1016/j.bbrc.2012.04.087
  • Peer Reviewed
• [Journal Article] Ezetimibe reduces fatty acid quantity in liver and decreased inflammatory cell infiltration and improved NASH in medaka model (2012)
  • Author(s): Oishi T, Terai S, Kuwashiro S, Fujisawa K, Matsumoto T, Nishina H, Sakaida I
  • Journal Title: Biochem Biophys Res Commun. Volume: in press
  • Peer Reviewed
• [Journal Article] Senescence marker protein 30 (SMP30)/regucalcin (RGN) expression decreases with aging, acute liver injuries and tumors in zebrafish. (2011)
  • Author(s): Fujisawa K, Terai S, Hirose Y, Takami T, Yamamoto N, Sakaida I.
  • Journal Title: Biochem Biophys Res Commun. Volume: 414 Pages: 331-336
  • Peer Reviewed
• [Presentation] メダカおよびマウスを用いた非アルコール性脂肪肝炎の病態研究および薬物スクリーニング (2013)
  • Author(s): 寺井 崇二
  • Organizer: 第60回日本実験動物学会総会シンポジウム(複数のリソースを効率的に用いた研究への招待 NBRP実験動物リソースシンポジウム)
  • Place of Presentation: つくば国際会議場(茨城)
  • Year and Date: 2013-05-15
• [Presentation] メダカおよびマウスを用いた非アルコール性脂肪肝炎の病態研究および薬物スクリーニング (2013)
  • Author(s): 寺井 崇二
  • Organizer: 第60回日本実験動物学会総会シンポジウム（複数のリソースを効率的に用いた研究への招待 NBRP実験動物リソースシンポジウム）
  • Place of Presentation: つくば国際会議場(茨城）
  • Invited
• [Presentation] SMP30/Regucalcin is a useful marker for liver tumor (2011)
  • Author(s): Koichi Fujisawa, Shuji Terai, Taro Takami, Naoki Yamamoto, Isao Sakaida
  • Organizer: ISHSR
  • Place of Presentation: イタリア フィッレンッエ
• [Presentation] HHM(Maid) is a specific regulator to inhibit the generation of liver tumor and progression of liver fibrosis-From the studyof CCl4 induced Maid KO mice and DEN exposure HHM Tg medaka fish- (2011)
  • Author(s): Shuji Terai, Naoki Yamamoto, Koichi Fujisawa, Tomoaki Murata, Isao Sakaida
  • Organizer: The Liver Meeting 2011
  • Place of Presentation: アメリカ サンフランシスコ