| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
The central nervous system generates spontaneous activities, which are thought to contribute to information processing; however, its function is not fully understood. In this study, to reveal its nature, we performed whole-brain imaging of C. elegans at cellular resolution by using 4D imaging system that we have designed and developed. Analysis of temporal changes in ratio of the individual neurons revealed a synchronized rhythmic activity in multiple neurons under non-stimulus condition. By frequency analysis indicated a major component is close to rhythm of switching forward-backward locomotion, implying that the rhythm component may function as a pacemakers or pattern generators for the locomotion. On the other hand, a gap-junction coding gene inx-7 mutation reduced spontaneous activity, implying gap-junctions may be involved in the pacemaker.
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