Functions of the non-clustered protocadherins in the neurogenesis and organogenesis

• Project/Area Number: 24590231
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Gunma University
• Principal Investigator: MURAKAMI Tohru 群馬大学, 医学(系)研究科(研究院), 准教授 (10239494)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 発生学・形態形成学 / 発生生物学 / 細胞接着因子 / プロトカドヘリン / ゼブラフィッシュ / in situハイブリダイゼーション / ドミナントネガティブ変異体 / アンチセンスMorpholino / protocadherin / cadherin / cell adhesion / eye development / Danio rerio / zebrafish

Outline of Final Research Achievements

The expression and functional analyses of a protocadherin Pcdh10a in zebrafish embryos indicated that it is expressed in the lens primordium and plays a significant role in its development. Comparative evidences is not known in its isoform Pcdh10b, and in other cell-adhesion molecules in other species. This diversity was phylogenetically hypothesized to have been introduced to zebrafish by the third-round whole genome duplication specific to the teleosts. Three-dimensional analysis technology is being developed for further analysis in larva and adult fish.

Research Products

• [Journal Article] Organization of organelles and VAMP-associated vesicular transport systems in differentiating skeletal muscle cells (2015)
  • Author(s): Tajika, Y., Takahashi, M., Ueno, H., Murakami, T., & Yorifuji, H
  • Journal Title: Anatomical Science International Volume: 90 Issue: 1 Pages: 33-39
  • DOI: 10.1007/s12565-014-0266-6
  • Peer Reviewed
• [Journal Article] Anterior cruciate ligament deterioration correlates with patella osteoarthritis (2014)
  • Author(s): Iriuchishima, T., Ryu, K., Aizawa, S., Yorifuji, H., Ohyama, T., & Fu, F. H.
  • Journal Title: International Orthopaedics Volume: 38 Issue: 4 Pages: 741-746
  • DOI: 10.1007/s00264-013-2141-9
  • Peer Reviewed
• [Journal Article] Vesicular transport system in myotubes: ultrastructural study and signposting with vesicle-associated membrane proteins (2014)
  • Author(s): Yuki Tajika, Maiko Takahashi, Astrid Feinisa Khairani, Hitoshi Ueno, Tohru Murakami & Hiroshi Yorifuji
  • Journal Title: Histochemistry and Cell Biology Volume: 141 Issue: 4 Pages: 441-454
  • DOI: 10.1007/s00418-013-1164-z
  • Peer Reviewed
• [Journal Article] 人体解剖とCTの統合による先駆的医学教育 (2014)
  • Author(s): Murakami, T., Tajika, Y., Ueno, H., & Yorifuji, H.
  • Journal Title: Innervision Volume: 29 Pages: 2-5
  • NAID: 120005624187
• [Journal Article] Filamentous structures in skeletal muscle: anchors for the subsarcolemmal space (2014)
  • Author(s): Astrid Feinisa Khairani, Yuki Tajika, Maiko Takahashi, Hitoshi Ueno, Tohru Murakami, Arifin Soenggono & Hiroshi Yorifuji
  • Journal Title: Medical Molecular Morphology Volume: e-published Issue: 1 Pages: 1-12
  • DOI: 10.1007/s00795-014-0070-3
  • Peer Reviewed
• [Journal Article] An Integrated Teaching Method of Gross Anatomy and Computed Tomography Radiology (2013)
  • Author(s): Tohru Murakami, Yuki Tajika, Hitoshi Ueno, Sachiko Awata, Satoshi Hirasawa, Maki Sugimoto, Yoshihiko Kominato, Yoshito Tsushima, Keigo Endo, & Hiroshi Yorifuji
  • Journal Title: Anatomical Sciences Education Volume: (e-published) Issue: 6 Pages: 438-449
  • DOI: 10.1002/ase.1430
  • Peer Reviewed
• [Journal Article] ACL footprint size is correlated with the height and area of the lateral wall of femoral intercondylar notch. (2013)
  • Author(s): Iriuchishima, T., Shirakura, K., Yorifuji, H., Aizawa, S., Murakami, T., & Fu, F. H.
  • Journal Title: Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA Volume: 21 Issue: 4 Pages: 789-796
  • DOI: 10.1007/s00167-012-2044-0
  • Peer Reviewed
• [Journal Article] The localization of VAMP5 in skeletal and cardiac muscle (2013)
  • Author(s): Maiko Takahashi, Yuki Tajika, Astrid Feinisa Khairani, Hitoshi Ueno, Tohru Murakami & Hiroshi Yorifuji
  • Journal Title: Histochemistry and Cell Biology Volume: 139 Issue: 4 Pages: 573-582
  • DOI: 10.1007/s00418-012-1050-0
  • Peer Reviewed
• [Journal Article] Alterations of biochemical marker levels and myonuclear numbers in rat skeletal muscle after ischemia-reperfusion. (2012)
  • Author(s): Itoh M
  • Journal Title: Mol Cell Biochem. Volume: 373 Issue: 1-2 Pages: 11-18
  • DOI: 10.1007/s11010-012-1470-0
  • Peer Reviewed
• [Journal Article] OsiriXによるAiと解剖実習の統合 (2012)
  • Author(s): 村上 徹
  • Journal Title: INNERVISION Volume: 27 Pages: 20-21
• [Presentation] 3D解剖映像配信システムによる解剖学実習支援 (2015)
  • Author(s): 村上 徹, 多鹿友喜, 上野仁之, 依藤 宏
  • Organizer: 第120回日本解剖学会総会・全国学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 2015-03-23
• [Presentation] 下肢解剖の着眼点 (2015)
  • Author(s): 村上 徹
  • Organizer: 第120回日本解剖学会総会・全国学術集会
  • Place of Presentation: 神戸国際会議場・展示場（兵庫県神戸市）
  • Year and Date: 2015-03-22
  • Invited
• [Presentation] Anatomy in Clay (R)－塑像による解剖学英語教育 (2014)
  • Author(s): 村上 徹, 多鹿友喜, 上野仁之, 依藤 宏, Deanna Clause 小材
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 自治医科大学
• [Presentation] 群馬大学におけるCTを用いた解剖学教育体制と放射線科診断医としての取り組み. (2014)
  • Author(s): 粟田さち子, 依藤 宏, 村上 徹, 多鹿 友喜, 上野 仁之, 小湊慶彦, 平澤 聡, 小林 進, 島田 健裕, 徳江浩, 対馬義人
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 自治医科大学
  • Invited
• [Presentation] 群馬大学における画像教育の基礎との統合による解剖実習. (2014)
  • Author(s): 依藤 宏, 村上 徹, 多鹿 友喜, 上野 仁之, 鹿田 光明, 森村 吉博
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 自治医科大学
  • Invited
• [Presentation] 骨格筋における中間径フィラメントsyneminの役割 (2014)
  • Author(s): 上野仁之, 佐藤真人, 高橋麻衣子, Astrid F. Khairani, 多鹿友喜, 村上 徹, 依藤 宏,
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 自治医科大学
• [Presentation] 筋管細胞に発現するVAMP分布と発達過程T管との比較 (2014)
  • Author(s): 多鹿友喜, 高橋麻衣子, Astrid F. Khairani, 上野仁之, 村上 徹, 依藤 宏
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 自治医科大学
• [Presentation] 気管支におけるVAMP5の発現と分布 (2014)
  • Author(s): 高橋麻衣子, 多鹿友喜, Astrid F. Khairani, 上野仁之, 村上 徹, 依藤 宏
  • Organizer: 第119回日本解剖学会総会・全国学術集会
  • Place of Presentation: 自治医科大学
• [Presentation] 骨格筋形成過程における細胞内膜系の電子顕微鏡観察 (2013)
  • Author(s): 多鹿 友喜, 高橋 麻衣子, Khairani Astrid, 上野 仁之, 村上 徹, 依藤 宏
  • Organizer: 第118会日本解剖学会総会・全国学術集会
  • Place of Presentation: 香川県高松市
• [Presentation] 心筋におけるVAMP アイソフォームの発現と分布 (2013)
  • Author(s): 高橋 麻衣子, 多鹿 友喜, Khairani Astrid, 上野 仁之, 村上 徹, 依藤 宏
  • Organizer: 第118会日本解剖学会総会・全国学術集会
  • Place of Presentation: 香川県高松市
• [Presentation] 中間径フィラメントsyneminの分子遺伝学的解析 (2013)
  • Author(s): 上野 仁之, 佐藤 真人, 高橋 麻衣子, Astrid Khairani, 多鹿 友喜, 村上 徹, 依藤 宏
  • Organizer: 第118会日本解剖学会総会・全国学術集会
  • Place of Presentation: 香川県高松市
• [Remarks] Protocols
  • URL: http://anatomy.med.gunma-u.ac.jp/protocols/
• [Remarks] Anatomy
  • URL: http://anatomy.med.gunma-u.ac.jp