Pathopysiology of fuluminant type 1 diabetes and development of its new therapy
| Project/Area Number |
24591320
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Metabolomics
|
|---|
| Research Institution | University of Yamanashi |
|---|
Principal Investigator |
ENDO Toyoshi 山梨大学, 総合研究部, その他 (00152017)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KANESHIGE Masahiro 山梨大学, 総合研究部, 助教 (20377518)
KOBAYASHI Tetsuro 公益財団法人冲中記念成人病研究所, 研究室, 研究員 (30113442)
TANAKA Syoichiro 山梨大学, 総合研究部, 助教 (70377521)
TAKIZAWA Soichiro 山梨大学, 総合研究部, 助教 (80456467)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
|---|
| Keywords | アミラーゼ / 自己免疫性膵炎 / 劇症１型糖尿病 / ２型糖尿病 / ELISA / 劇症1型糖尿病 / Amylaseα-2A / 自己抗体 / 慢性膵炎 / DNA免疫 / DNA 免疫 |
|---|
| Outline of Final Research Achievements |
To study epitopes of the autoantibody against AMY, we performed an ELISA using synthetic peptides corresponding to the C-terminal of AMY as antigens, and found that peptide 7 (P7) showed antigenicity to the autoantibody.Using P7 as an antigen, we screened for the autoantibody in patients with pancreas diseases. we screened for AMY autoantibody in AIP using the P7-coated ELISA plate (P7-ELISA). Eighteen out of 32 patients with AIP from our cohort were positive, suggesting that the results of P7-ELISA were more specific than those with C-AMY-ELISA. Of the 86 T2DM patients, 14 (16%) patients were positive for AMY autoantibodies. Fasting serum C-peptide levels of antibody positive-T2DM patients were significantly lower than that of antibody negative patients.
We immunized P7 peptide into subcutaneous tissues of mouse biweekly.
|
Report
(4 results)
Research Products
(7 results)
-
[Journal Article] Distinct cell clusters touching islet cells induce islet cell replication in association with over-expression of Regenerating Gene (REG) protein in fulminant type 1 diabetes.2014
Author(s)
Aida K, Saitoh S, Nishida Y, Yokota S, Ohno S, Mao X, Akiyama D, Tanaka S, Awata T, Shimada A, Oikawa Y, Shimura H, Furuya F, Takizawa S, Ichijo M, Ichijo S, Itakura J, Fujii H, Hashiguchi A, Takasawa S, Endo T, Kobayashi T.
-
Journal Title
Related Report
-
-
-
-
-
-
