Selective Development of Myogenic Mesenchymal Cells from Human Embryonic and Induced Pluripotent Stem Cells
| Project/Area Number |
24591508
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kyoto University |
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Principal Investigator |
KATO Takeo 京都大学, 医学(系)研究科(研究院), 助教 (60422945)
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| Co-Investigator(Kenkyū-buntansha) |
AWAYA Tomonari (20589593)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | iPS細胞 / 骨格筋分化誘導 / 筋ジストロフィー / 疾患特異的iPS細胞 / 骨格筋細胞 |
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| Outline of Final Research Achievements |
we developed a novel stepwise culture method for the selective expansion of mesenchymal cells from
cell aggregations called embryoid bodies. These mesenchymal cells, which were obtained by dissociation and re-cultivationof embryoid bodies, uniformly expressed CD56 and the mesenchymal markers CD73, CD105, CD166, and CD29, and finally differentiated into mature myotubes in vitro. Furthermore, these myogenic mesenchymal cells exhibited stable long-term engraftment in injured muscles of immunodeficient mice in vivo
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Report
(4 results)
Research Products
(3 results)
