Regulation of drug permeability of the blood-inner ear barrier by RNA interference method
| Project/Area Number |
24592548
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kobe University |
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Principal Investigator |
TANIMOTO Hitoshi 神戸大学, 医学(系)研究科(研究院), 研究員 (50362786)
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| Co-Investigator(Kenkyū-buntansha) |
OTSUKI Naoki 神戸大学, 医学(系)研究科(研究院), 准教授 (40343264)
YAMASHITA Daisuke 神戸大学, 医学部附属病院, 准教授 (60306785)
KATSUNUMA Sayaka 神戸大学, 医学部附属病院, 助教 (80457043)
FUJITA Takeshi 神戸大学, 医学部附属病院, 助教 (90533711)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 内耳 / 血液-内耳関門 / 感音難聴 / RNA干渉 / claudin-5 / クローディン / ドラッグデリバリーシステム / 血液ー内耳関門 / ＲＮＡ干渉 |
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| Outline of Final Research Achievements |
By RNA interference method, it is possible to suppress the transcription of protein structure of the blood-inner ear barrier, and to adjust the blood-inner ear barrier barrier mechanism. For the mouse to adjust the blood-inner ear barrier barrier mechanism, intravenous drug to the systemic administration, we want to prove that the drug has reached the inner ear. It is acoustic trauma modeling gives a huge acoustic trauma, and is administered inhibit RNA interference agent through the intravenous of which is the main protein claudin-5 transcription of the blood-inner ear barrier. Then, intravenously to be administered through the brain-derived neurotrophic factor (BDNF) as a neuroprotective agent for the purpose of inner ear disorder cell regeneration. And a control mouse morphological, we went electrophysiological comparison.
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Report
(4 results)
Research Products
(4 results)
