| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Japanese radish (Raphanus sativus L.) is a seed-vernalization-type plant requiring chilling for flowering．It is also known that exposure of seeds to high temperature has a devernalization effect. The purpose of this study is to clarify the molecular aspects of vernalization and devernalization of Japanese radish by investigating the expressions of some flowering-related genes. In 'Hayabutori-syogoinn', the expression level of RsFLC decresesed with increased duration of chilling and thereafter the expression levels of RsFT and RsSOC1 increased prior to flowering. Exposure of vernalized seeds with 9 days' chilling to high temperature caused devernalization and the RsFLC level was restored a little. Close relations between vernalized or devernalized status and the expression level of RsFLC were observed in 'Hayabutori-syogoinn' and 'Wakayama', but in 'Tokinashi' the low expression levels of RsFLC after exposure to 18 days' chilling did not result in flowering.
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