Analysis of male germ cell specific GPI-anchored proteins using gene-manipulated mice
| Project/Area Number |
24700430
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 雄性生殖細胞 / GPIアンカータンパク質 / 精子受精能 / 遺伝子組換えマウス / 受精 / 精子 / 遺伝子改変マウス |
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| Outline of Final Research Achievements |
Germ cells are the only cells that can carry genetic information on to the next generation. I focused on the male germ cell specific glycosylphosphatidylinositol (GPI)-anchored protein complexes, TEX101 and LY6K, and studied their physiological functions using gene-manipulated mice.
I showed that these GPI-anchored protein complexes are required for sperm migrating ability into the oviduct and subsequent male fertility (PNAS. 2013, BOR. 2014). TEX101 and LY6K protein complexes interacted with the sperm membrane protein ADAM3 in mouse testes. However, spermatozoa from LY6K-deficient mice had no aberrance in ADAM3. Thus, the no effect on ADAM3 in LY6K-deficient mice is indicative of an as yet undefined pathway in the mouse.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Induction of DNA Methylation by Artificial piRNA Production in Male Germ Cells2015
Author(s)
Itou D, Shiromoto Y, Shin-Ya Y, Ishii C, Nishimura T, Ogonuki N, Ogura A, Hasuwa H, Fujihara Y, Kuramochi-Miyagawa S, Nakano T.
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Journal Title
Current biology
Volume: 25
Issue: 7
Pages: 901-906
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes2014
Author(s)
Mashiko D, Young SA, Muto M, Kato H, Nozawa K, Ogawa M, Noda T, Kim YJ, Satouh Y, Fujihara Y, Ikawa M.
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Journal Title
Development, Growth & Differentiation
Volume: 56
Issue: 1
Pages: 122-129
DOI
Related Report
Peer Reviewed
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