Structural and functional diversities of mammalian cilia
| Project/Area Number |
24770183
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | University of Yamanashi |
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Principal Investigator |
NARITA Keishi 山梨大学, 医学工学総合研究部, 講師 (50452131)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 繊毛 / 多様性 / TRPV4 / イメージング / 脈絡叢 / 上衣細胞 / 脳脊髄液 / 上皮細胞 / ライブセルイメージング / シグナル伝達 |
|---|
| Research Abstract |
1) We have investigated the molecular mechanism of ciliary number regulation in mammalian cells. Based on transciptome analysis and the following knockdown experiments, we identified one novel gene of interest. We have constructed CRISPR/Cas9 plasmid to generate knockout mouse of this gene. 2) To monitor the cellular event of multiciliogenesis, we have generated lentiviral constructs to label the ciliary shaft and the basal body by fluorescent proteins. 3) We have investigated the unique functional transition of cilia in choroid plexus epithelial cells (CPECs) from the prenatal period. 4) We have analyzed the ciliary localization and functional significance of TRPV4 in CPECs.
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Report
(3 results)
Research Products
(15 results)
