| Project/Area Number |
24790186
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KIMURA Shunsuke 北海道大学, 大学院医学研究科, 助教 (40444525)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 細胞間相互作用 / 膜ナノチューブ / パイエル板 / M細胞 |
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| Outline of Final Research Achievements |
M-Sec is a cytoplasmic protein expressed by macrophages and intestinal microfold cells (M cells). We have previously reported that M-Sec in macrophage induces plasma membrane deformation resulting in membrane nanotube; however function of M-Sec in M cells as well as the existence of membrane nanotubes has been largely unknown. M cells are specialized epithelial cells reside in follicle-associated epithelium of Peyer’s patches, and have uptake capacity of luminal macromolecules. Differentiation of M cells is thought to be regulated by the interaction with stromal cells underneath epithelial basement membrane. In the present study, we found that M cell had membrane protrusions on the basolateral membrane. The protrusions were elongated across basement membrane and reached to stromal cells. On the other hand, M-Sec KO mice did not show clear phenotype in the protrusion formation and M-cell differentiation, suggesting the probability that other molecules compensate the function of M-Sec.
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