Effects and molecular mechanisms of glioblastoma cell motility in carbon ion beam irradiation

• Project/Area Number: 24791275
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Gunma University
• Principal Investigator: YOSHIDA Yukari 群馬大学, 重粒子線医学推進機構, 助教 (90431717)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 遊走 / 脳腫瘍 / 炭素イオン線 / 重粒子線 / 悪性脳腫瘍

Outline of Final Research Achievements

We investigated the relationship between cell invasion and irradiation. Human glioblastoma (GBM) cell lines were irradiated with X-rays or carbon ion beams(C-ions). Not only X-rays but C-ions irradiation increased cell motility at 24 hours after irradiation. C-ions irradiated cells were more migrative than X-rays irradiated cells. Increase of cell migration by irradiation was linear energy transfer (LET)-independent manner.
Suppression of O6-methylguanine-DNA methyltransferase (MGMT) expression increased migration of GBM cells. These results suggest that depletion of MGMT is associated with increased cell migration.

Research Products

• [Journal Article] Radiosensitizing effect of carboplatin and paclitaxel to carbon-ion beam irradiation in the non-small-cell lung cancer cell line H460. (2015)
  • Author(s): Kubo N, Noda SE, Takahashi A, Yoshida Y, Oike T, Murata K, Musha A, Suzuki Y, Ohno T, Takahashi T, Nakano T.
  • Journal Title: J Radiat Res. Volume: 56 Issue: 2 Pages: 229-238
  • DOI: 10.1093/jrr/rru085
  • Peer Reviewed
• [Journal Article] Nonhomologous end-joining repair plays a more important role than homologous recombination repair in defining radiosensitivity after exposure to high-LET radiation. (2014)
  • Author(s): Takahashi A, Kubo M, Ma H, Nakagawa A, Yoshida Y, Isono M, Kanai T, Ohno T, Furusawa Y, Funayama T, Kobayashi Y, Nakano T.
  • Journal Title: Radiat Res. Volume: 182 Issue: 3 Pages: 338-344
  • DOI: 10.1667/rr13782.1
  • Peer Reviewed
• [Journal Article] Carbon-Ion Beam Irradiation Kills X-Ray-Resistant p53-Null Cancer Cells by Inducing Mitotic Catastrophe (2014)
  • Author(s): Amornwichet N, Oike T, Shibata A, Ogiwara H, Tsuchiya N, Yamauchi M, Saitoh Y, Sekine R, Isono M, Yoshida Y, Ohno T, Kohno T, Nakano T
  • Journal Title: PLoS One Volume: 9 Issue: 12 Pages: e115121-e115121
  • DOI: 10.1371/journal.pone.0115121
  • NAID: 120006986291
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Comparison of the radiosensitivities of neurons and glial cells derived from the same rat brain. (2014)
  • Author(s): Kudo S, Suzuki Y, Noda SE, Mizui T, Shirai K, Okamoto M, Kaminuma T, Yoshida Y, Shirao T, Nakano T.
  • Journal Title: Exp Ther Med. Volume: 8 Pages: 754-758
  • Peer Reviewed
• [Journal Article] Increase in cell motility by carbon ion irradiation via the Rho signaling pathway and its inhibition by the ROCK inhibitor Y-27632 in lungadenocarcinoma A549 cells. (2014)
  • Author(s): Kazutoshi Murata, Shin-ei Noda, Takahiro Oike, Akihisa Takahashi, Yukari Yoshida, Yoshiyuki Suzuki, Tatsuya Ohno, Tomoo Funayama, Yasuhiko Kobayashi, Takeo Takahashi, Takashi Nakano
  • Journal Title: J Radiat Res. Volume: 55 Issue: 4 Pages: 658-664
  • DOI: 10.1093/jrr/rru002
  • Peer Reviewed / Open Access
• [Journal Article] Long-term pathological and immunohistochemical features in the liver after intraoperative whole-liver irradiation in rats. (2014)
  • Author(s): Imaeda M, Ishikawa H, Yoshida Y, Takahashi T, Ohkubo Y, Musha A, Komachi M, Nakazato Y, Nakano T.
  • Journal Title: J Radiat Res. Volume: 55 Issue: 4 Pages: 665-673
  • DOI: 10.1093/jrr/rru005
  • Peer Reviewed / Open Access
• [Journal Article] Increase in cell motility by carbon ion irradiation via the Rho signaling pathway and its inhibition by the ROCK inhibitor Y-27632 in lung adenocarcinoma A549 cells. (2014)
  • Author(s): Murata K, Noda SE, Oike T, Takahashi A, Yoshida Y, Suzuki Y, Ohno T, Funayama T, Kobayashi Y, Takahashi T, Nakano T
  • Journal Title: J Radiat Res Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Long-term pathological and immunohistochemical features in the liver after intraoperative whole-liver irradiation in rats. (2014)
  • Author(s): Imaeda M, Ishikawa H, Yoshida Y, Takahashi T, Ohkubo Y, Musha A, Komachi M, Nakazato Y, Nakano T.
  • Journal Title: J Radiat Res Volume: 印刷中
  • Peer Reviewed
• [Journal Article] X irradiation changes dendritic spine morphology and density through reduction of cytoskeletal proteins in mature neurons. (2013)
  • Author(s): Shirai K, Mizui T, Suzuki Y, Okamoto M, Hanamura K, Yoshida Y, Hino M, Noda SE, Al-jahdari WS, Chakravarti A, Shirao T, Nakano T.
  • Journal Title: Radiat Res. Volume: 179 Issue: 6 Pages: 630-6
  • DOI: 10.1667/rr3098.1
  • Peer Reviewed
• [Journal Article] Evaluation of the relative biological effectiveness of carbon ion beams in the cerebellum using the rat organotypic slice culture system (2012)
  • Author(s): Yoshida Y, Suzuki Y, Al-Jahdari WS, Hamada N, Funayama T, Shirai K, Katoh H, Sakashita T, Kobayashi Y, Nakano T
  • Journal Title: J Radiat Res Volume: 53 Pages: 87-92
  • NAID: 10030023646
  • Peer Reviewed
• [Journal Article] Synergistic effect of heat shock protein 90 inhibitor, 17-allylamino-17-demethoxygeldanamycin and X-rays, but not carbon-ion beams, on lethality in human oral squamous cell carcinoma cells (2012)
  • Author(s): Musha A, Yoshida Y, Takahashi T, Ando K, Funayama T, Kobayashi Y, Negishi A, Yokoo S, Nakano T
  • Journal Title: J Radiat Res Volume: 53 Pages: 545-550
  • Peer Reviewed
• [Presentation] 重粒子線がん治療のための生物学的基礎研究 (2015)
  • Author(s): 吉田由香里
  • Organizer: 茨城大学Quantum Medicine研究会
  • Place of Presentation: 茨城大学、水戸
  • Year and Date: 2015-03-01
  • Invited
• [Presentation] X-irradiation induces acute depolymerization of microfilaments and structural changes in neurites of cultured hippocampal neurons. (2015)
  • Author(s): Yukari Yoshida, Mizuki Hino, Yoshiyuki Suzuki, Kenji Hanamura, Katsuyuki Shirai, Tomoaki Shirao, Takashi Nakano
  • Organizer: 第5回国際放射線神経生物学会大会
  • Place of Presentation: 高崎シティーギャラリー、高崎
  • Year and Date: 2015-02-21
• [Presentation] 重粒子線がん治療の至適分割照射法開発の為の基礎研究 (2014)
  • Author(s): 吉田由香里、安藤興一、小池幸子、鵜澤玲子、磯野真由、池田裕子、溝端健亮、金井達明、大野達也、中野隆史
  • Organizer: 日本放射線影響学会第57回大会
  • Place of Presentation: 鹿児島県民交流センター、鹿児島
  • Year and Date: 2014-10-01 – 2014-10-03
  • Invited
• [Presentation] LET dependence of cell survival parameters in normal human skin cells (2014)
  • Author(s): Yukari Yoshida, Mayu Isono, Kensuke Mizohata, Tomoko Yakoh, Koichi Ando, Tatsuya Ohno, Takashi Nakano, Tatsuaki Kanai
  • Organizer: PTCOG53
  • Place of Presentation: Shanghai, China
  • Year and Date: 2014-06-12 – 2014-06-14
• [Presentation] 放射線感受性におよぼすDNA二本鎖切断修復のLET依存的な効果. (2013)
  • Author(s): 高橋昭久, 吉田由香里, 古澤佳也, 大野達也, 中野隆史
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 神経膠芽腫細胞に対する炭素線とtemozolomide併用に関する生物学的効果の検討 (2013)
  • Author(s): 吉田由香里, 石内勝吾, 鈴木義行, 高橋昭久, 大野達也, 中野隆史
  • Organizer: 第3回国際放射線神経生物学学会大会
  • Place of Presentation: 沖縄
• [Presentation] Biological gain of fractionated carbon-ion radiotherapy for tumor growth delay and crypt survival (2012)
  • Author(s): Y Yoshida, K Ando, S Koike, A Uzawa, K Ando, K Murata, Y Yoshimoto, A Musha, N Kubo, H Kawamura, Y Matsumoto, R Hirayama, Y Suzuki, T Takahashi, T Ohno, T Nakano
  • Organizer: Particle Therapy Co-Operative Group (PTCOG) 51
  • Place of Presentation: 韓国
• [Presentation] 正常組織と腫瘍の炭素線分割照射効果の比較 (2012)
  • Author(s): 吉田由香里, 安藤興一, 小池幸子, 鵜澤玲子, 安藤謙, 村田和俊, 吉本由哉, 武者篤, 久保亘輝, 河村英将, 松本孔貴, 平山亮一,古澤佳也, 高橋健夫, 大野達也, 中野隆史
  • Organizer: 第47回群馬放射線腫瘍研究会
  • Place of Presentation: 群馬
• [Presentation] 炭素イオン線における正常組織と腫瘍の分割照射効果の比較 (2012)
  • Author(s): 吉田由香里, 安藤興一, 小池幸子, 鵜澤玲子, 安藤謙, 村田和俊, 吉本由哉, 古澤佳也, 大野達也, 中野隆史
  • Organizer: 第55回日本放射線影響学会
  • Place of Presentation: 仙台
• [Presentation] 炭素イオン線分割照射効果に関する正常組織と腫瘍の比較 (2012)
  • Author(s): 吉田由香里, 安藤興一, 小池幸子, 鵜澤玲子, 安藤謙, 村田和俊, 吉本由哉, 武者篤, 久保亘輝, 河村英将, 松本孔貴, 平山亮一, 鈴木義行, 古澤佳也, 高橋健夫, 大野達也, 中野隆史
  • Organizer: 第50回日本放射線腫瘍学会生物部会学術大会
  • Place of Presentation: 沖縄
• [Presentation] Biophysical quality assurance for Treatment Planning of Carbon-Ion Therapy (2012)
  • Author(s): Y Yohida
  • Organizer: KHIMA seminar on heavy-ion radiotherapy
  • Place of Presentation: 韓国
  • Invited
• [Presentation] 非小細胞肺癌に対する炭素線照射とカルボプラチン、パクリタキセル併用の基礎的検討
  • Author(s): 久保亘輝、野田真永、高橋昭久、村田和俊、武者篤、永島潤、吉田由香里、大野達也、高橋健夫、中野隆史
  • Organizer: 第51回日本癌治療学会学術集会
  • Place of Presentation: 国立京都国際会館・グランドプリンスホテル京都
• [Presentation] Commissioning Status of Layer-Stacking Irradiation Method at Gunma University
  • Author(s): Yusa K, Yoshida Y, et al.
  • Organizer: PTCOG52
  • Place of Presentation: Essen, Germany
• [Presentation] Report of a biological experiment with scanning beam in GHMC
  • Author(s): A Takeshita, Y Yoshida, et al
  • Organizer: 105JSMP
  • Place of Presentation: パシフィコ横浜
• [Book] 臨床放射線生物学の基礎 原著４版 (2013)
  • Author(s): 監訳 安藤興一 中野隆史 （8, 13, 22章吉田由香里 担当）
  • Total Pages: 375
  • Publisher: NPO法人 放射線医療国際協力推進機構