Meiotic G2/M-phase transition in response to maturation-inducing hormone

• Project/Area Number: 25291043
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Ochanomizu University
• Principal Investigator: Kishimoto Takeo お茶の水女子大学, サイエンス&エデュケーションセンター, 客員教授 (00124222)
• Co-Investigator(Kenkyū-buntansha): OKUMURA Eiichi 東京工業大学, 大学院生命理工学研究科, 助教 (00323808) ; TACHIBANA Kazunori 東京工業大学, バイオ研究基盤支援総合センター, 准教授 (60212031)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2015: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2013: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 細胞周期 / ホルモン / 分裂期 / 卵成熟 / Cdk1 / ホルモン受容体 / 細胞内情報伝達 / 閾値

Outline of Final Research Achievements

Immature oocytes arrest at the G2/M-phase border, and the maturation-inducing hormone stimulates release of this arrest via activation of cyclin B-Cdk1, the M-phase governor. Here we investigated the intracellular signal transduction mechanism underlying this release of the cell cycle arrest. We identified molecular components of the binding protein of the maturation-inducing hormone which is located on the oocyte surface. We further revealed the presence of the self-calming system that cancels the stimulus of insufficient levels of maturation-inducing hormone, leading to prevention of an unexpected activation of cyclin B-Cdk1.

Research Products

• [Journal Article] Entry into mitosis: a solution to the decades-long enigma of MPF. (2015)
  • Author(s): Kishimoto, T.
  • Journal Title: Chromosoma Volume: 124 Issue: 4 Pages: 417-428
  • DOI: 10.1007/s00412-015-0508-y
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cyclin B-Cdk1 inhibits protein phosphatase PP2A-B55 via a Greatwall kinase-independent mechanism. (2014)
  • Author(s): Okumura, E., Morita, A., Wakai, M., Mochida, S., Hara, M., and Kishimoto, T.
  • Journal Title: J. Cell Biol. Volume: 204 Issue: 6 Pages: 881-889
  • DOI: 10.1083/jcb.201307160
  • Peer Reviewed
• [Journal Article] The Mos-MAPK pathway regulates Diaphanous-related formin activity to drive cleavage furrow closure during polar body extrusion in starfish oocytes. (2013)
  • Author(s): Ucar, H., Tachibana, K., and Kishimoto, T.
  • Journal Title: J. Cell Sci. Volume: 126 Pages: 5153-5165
  • DOI: 10.1242/jcs.130476
  • Peer Reviewed
• [Presentation] Gβγ依存的な新規経路によるAktの基質のリン酸化昂進 (2015)
  • Author(s): 平岡大作、岸本健雄
  • Organizer: 第38回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2015-12-01
• [Presentation] To activate, or not to activate cyclin B-Cdk1: that is the question of the oocyte at G2/M-phase border. (2015)
  • Author(s): Daisaku Hiraoka and Takeo Kishimoto
  • Organizer: EMBO Workshop on the Cell Cycle
  • Place of Presentation: Budapest, Hungary
  • Year and Date: 2015-09-04
  • Int'l Joint Research
• [Presentation] To resume, or not to resume meiosis: how do starfish oocytes make a decision? (2015)
  • Author(s): Daisaku Hiraoka and Takeo Kishimoto
  • Organizer: Oocyte Maturation and Fertilization Meeting IV
  • Place of Presentation: 東北大学浅虫臨海実験所
  • Year and Date: 2015-06-15
  • Int'l Joint Research / Invited
• [Presentation] Autoregulatory activation of cyclin B-Cdk1: Arpp19, but not its upstream Gwl, is essential at meiotic G2/M-phase transition in starfish oocytes. (2015)
  • Author(s): Eiichi Okumura, Atsushi Morita, Satoru Mochida, Masatoshi Hara, and Takeo Kishimoto
  • Organizer: Oocyte Maturation and Fertilization Meeting IV
  • Place of Presentation: 東北大学浅虫臨海実験所
  • Year and Date: 2015-06-15
  • Int'l Joint Research / Invited
• [Presentation] ヒトデ卵減数分裂再開時におけるAktの基質のリン酸化の制御機構 (2014)
  • Author(s): 平岡大作、森康剛、香月美穂、立花和則、岸本健雄
  • Organizer: 日本分子生物学会 第37回年会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Signalling module that generates a switch-like activation of cyclin B-Cdk1 at meiotic G2/M-phase transition. (2014)
  • Author(s): Takeo Kishimoto
  • Organizer: Jacques Monod Conference on "Cell cycle: bridging scales in cell division"
  • Place of Presentation: Roscoff, France
  • Year and Date: 2014-10-11 – 2014-10-15
• [Presentation] ヒトデ卵減数分裂再開時におけるcyclin B-Cdk1によるPP2A阻害因子Ensa/Arpp19の制御 (2014)
  • Author(s): 奥村英一、森田敦、持田悟、原昌稔、岸本健雄
  • Organizer: 日本動物学会 第85回大会
  • Place of Presentation: 東北大学、仙台
  • Year and Date: 2014-09-11 – 2014-09-14
• [Presentation] Starfish oocyte maturation (Key Note Talk) (2013)
  • Author(s): Takeo Kishimoto
  • Organizer: EMBO Workshop on “Oocyte Maturation and Fertilization: Lessons from Canonical and Emerging Models”
  • Place of Presentation: Banyuls-sur-Mer, France
  • Invited
• [Presentation] 卵細胞周期の制御機構：ＭＰＦの再考 (Plenary Lecture) (2013)
  • Author(s): 岸本健雄
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: ウインクあいち（愛知県産業労働センター）
  • Invited
• [Book] Methods in Mol. Biol., vol. 1128（Chap. 21を分担執筆） (2014)
  • Author(s): Okumura, E., Hara, M., and Kishimoto, T.
  • Total Pages: 347
  • Publisher: Springer Science+Business Media, New York
• [Remarks] 東工大・生命理工・立花研究室ホームページ（その中で岸本名誉教授として表示）
  • URL: http://www.cell-dev.bio.titech.ac.jp/home/index-j.html