| Project/Area Number |
25462767
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | National Center for Child Health and Development |
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Principal Investigator |
Nishina Sachiko 国立研究開発法人国立成育医療研究センター, 感覚器・形態外科部・眼科, 医員 (40237954)
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| Co-Investigator(Renkei-kenkyūsha) |
AZUMA Noriyuki 国立成育医療研究センター, 感覚器・形態・外科部眼科, 医長 (10159395)
NISHINA Hiroshi 東京医科歯科大学, 難治疾患研究所, 教授 (60212122)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 網膜神経節細胞 / 神経細胞 / iPS細胞 / ES細胞 / 先天眼異常 / 眼疾患 / 小児 / 再生医療 / 網膜神経細胞 / 網膜芽細胞腫 / 低分子化合物 / ケミカルバイオロジー |
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| Outline of Final Research Achievements |
In this study, we tried to establish to generate neuronal cells including self-induced retinal ganglion cells (RGCs). Results: 1) After development of the optic vesicle from the induced stem cell embryoid body in three-dimensional culture, conversion to two-dimensional culture, achieved by supplementation with BDNF, resulted in differentiation of RGCs; 2) We utilised a mouse embryonic stem cell differentiation system and a library of well-characterised drugs to identify these metabolic factors. We found that statins, which inhibit the mevalonate metabolic pathway, suppressed primitive streak formation in vitro and in vivo. These results may contribute to both basic and clinical science, including embryology, neurology, pathognomy, and treatment of various optic nerve diseases that threaten vision.
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