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In vivo imaging probes for visualization of activated osteoclasts

Research Project

Project/Area Number 25620133
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Bio-related chemistry
Research InstitutionOsaka University

Principal Investigator

KIKUCHI Kazuya  大阪大学, 工学(系)研究科(研究院), 教授 (70292951)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
KeywordspH感受性プローブ / 破骨細胞 / 二光子蛍光イメージング / BODIPY / ヒドロキシアパタイト / 低pH / pH感受性プローブ
Outline of Final Research Achievements

Osteoclasts are resorptive cells of bone and their activity is involved in the development of osteoporosis and rheumatoid arthritis. Elucidation of the mechanism of the bone resorption is highly important for the therapeutic applications. In this study, we developed a novel fluorescent probe “pHocas-3” that has low-pH sensitivity, high photostablity, and in vivo bone delivery efficiency. By administrating of this fluorescent probe in living mice, we achieved intravital fluorescence imaging of activated osteoclasts during a long time period using a two photon fluorescence microscopy. These results demonstrated that intravital fluorescence imaging using pHocas-3 will be a useful tool for investigating of the dynamics of activated osteoclasts in vivo.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 2013

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (3 results) (of which Invited: 3 results)

  • [Journal Article] Actavataqble 19F MRI nanoparticle probes for the detection of reducing environments2015

    • Author(s)
      Nkamura, T., Matsushita, H., Sugihara, F., Yoshioka, Y., Mizukami, S. and Kikuchi, K.
    • Journal Title

      Angewandte Chemi International Edition

      Volume: 54 Issue: 3 Pages: 1007-1010

    • DOI

      10.1002/anie.201409365

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intramolecular Long-distance Nucleophilic Reactions as a Rapid Fluorogenic Switch Applicable to Detection of Enzymatic Activity2015

    • Author(s)
      Reisuke BABA, Yuichiro HORI, Kazuya KIKUCHI
    • Journal Title

      Chem. Eur. J.

      Volume: 21 Issue: 12 Pages: 4695-4702

    • DOI

      10.1002/chem.201406093

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of a Fluorogenic Probe Based on a DNA Staining Dye for Continuous Monitoring of the Histone Deacetylase Reaction2014

    • Author(s)
      M. Minoshima, T. Matsumoto, and K. Kikuchi
    • Journal Title

      Anal. Chem.

      Volume: 86 Issue: 15 Pages: 7925-7930

    • DOI

      10.1021/ac501881s

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development of Luminescent Coelenterazine Derivatives Activatable by β-Galactosidase for Monitoring Dual Gene Expression2013

    • Author(s)
      Lindberg E, Mizukami S, Ibata K, Miyawaki A, Kikuchi K
    • Journal Title

      Chem. Eur. J.

      Volume: 19 Issue: 44 Pages: 14970-14976

    • DOI

      10.1002/chem.201302002

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Multifunctional Core-shell Silica Nanoparticles for Highly Sensitive ^<19>F MRI2013

    • Author(s)
      H. Matsushita, S. Mizukami, F. Sugihara, Y. Nakanishi, Y. Yoshioka & K. Kikuchi
    • Journal Title

      Angew. Chem. Int. Ed

      Volume: 52(in press) Issue: 4 Pages: 52-52

    • DOI

      10.1002/anie.201308500

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Intracellular Protein Labeling by Functional Probes with Tunable Chemical Switches2014

    • Author(s)
      Kazuya Kikuchi
    • Organizer
      Labeling and Nanoscopy
    • Place of Presentation
      Heidelberg, Gemany
    • Year and Date
      2014-09-24 – 2014-09-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Design, Synthesis and Biological Application of Molecular Imaging Probes with Tunable Chemical Switches2013

    • Author(s)
      Kazuya Kikuchi
    • Organizer
      AIMECS 2013
    • Place of Presentation
      Taiwan
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Design, Synthesis and Biological Application of in Vivo Imaging Probes with Tunable Chemical Switches2013

    • Author(s)
      Kazuya Kikuchi
    • Organizer
      ICMAT 2013
    • Place of Presentation
      Singapore
    • Related Report
      2013 Research-status Report
    • Invited

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Published: 2014-07-25   Modified: 2019-07-29  

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