| Project/Area Number |
25620133
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Bio-related chemistry
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
KIKUCHI Kazuya 大阪大学, 工学(系)研究科(研究院), 教授 (70292951)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
|
|---|
| Keywords | ｐH感受性プローブ / 破骨細胞 / 二光子蛍光イメージング / BODIPY / ヒドロキシアパタイト / 低pH / pH感受性プローブ |
|---|
| Outline of Final Research Achievements |
Osteoclasts are resorptive cells of bone and their activity is involved in the development of osteoporosis and rheumatoid arthritis. Elucidation of the mechanism of the bone resorption is highly important for the therapeutic applications. In this study, we developed a novel fluorescent probe “pHocas-3” that has low-pH sensitivity, high photostablity, and in vivo bone delivery efficiency. By administrating of this fluorescent probe in living mice, we achieved intravital fluorescence imaging of activated osteoclasts during a long time period using a two photon fluorescence microscopy. These results demonstrated that intravital fluorescence imaging using pHocas-3 will be a useful tool for investigating of the dynamics of activated osteoclasts in vivo.
|
