Study of the movement of damaged DNA

• Project/Area Number: 25640066
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: Hiroshima University
• Principal Investigator: TASHIRO Satoshi 広島大学, 原爆放射線医科学研究所, 教授 (20243610)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 相同組換え / 細胞核構造 / 転写反応

Outline of Final Research Achievements

In this study, we examined the movement of damaged DNA. For this purpose, we have established a method to induce DNA damage at a specific DNA sites and to visualize the region by FISH technique. We are now analyzing the numerous images obtained by these experiments.
We also found that over-expressed DNA repair protein RAD51 is accumulated at bundle like nuclear compartments. Immunofluorescence analysis revealed that RPA, a single strand binding protein, moved into the bundle like nuclear zone after induction of DNA damage. We are now preparing a manuscript including these new findings obtained by this project.

Research Products

• [Journal Article] Regulation of homologous recombinational repair by lain B1 in radiation-induced DNA damage (2015)
  • Author(s): Liu NA
  • Journal Title: FASEB J Volume: 29 Pages: 1-13
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Structural basis for ubiquitin recognition by ubiquitin-binding zinc finger of FAAP20 (2015)
  • Author(s): Toma, A, Takahashi, T. S., Sato, Y., Yamagata, A., Goto-Ito, S., Nakada, S., Fukuto, A., Horikoshi, Y., Tashiro, S., Fukai, S.
  • Journal Title: PLoS One Volume: 10 Issue: 3 Pages: 1-15
  • DOI: 10.1371/journal.pone.0120887
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Reorganization of Damaged Chromatin by the Exchange of Histone Variant H2A.Z-2. (2014)
  • Author(s): Nishibuchi I
  • Journal Title: Int J Radiat Oncol Biol Phys. Volume: 89 Issue: 4 Pages: 736-744
  • DOI: 10.1016/j.ijrobp.2014.03.031
  • Peer Reviewed / Open Access
• [Journal Article] Smoking cessation reverses DNA double-strand breaks in human mononuclear cells. (2014)
  • Author(s): Ishida M
  • Journal Title: PLoS One Volume: 9 Issue: 8 Pages: 1-9
  • DOI: 10.1371/journal.pone.0103993
  • Peer Reviewed / Open Access
• [Journal Article] Nap1 stimulates homologous recombination by RAD51 and RAD54 in higher-ordered chromatin containing histone H1. (2014)
  • Author(s): Machida, S., Takaku, M., Ikura, M., Sun, J., Suzuki, H., Kobayashi, W., Kinomura, A., Osakabe, A., Tachiwana, H., Horikoshi, Y., Fukuto, A., Matsuda, R., Ura, K., Tashiro, S., Ikura, T., and Kurumizaka, H.
  • Journal Title: Scientific Rep. Volume: 4 Issue: 1 Pages: 4863-4863
  • DOI: 10.1038/srep04863
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Activation of the SUMO modification system is required for the accumulation of RAD51 at sites containing DNA damage (2013)
  • Author(s): Shima H, Suzuki H, Sun J, Kono K, Shi L, Kinomura A, Horikoshi Y, Ikura T, Ikura M, Kanaar R, Igarashi K, Saitoh H, Kurumizaka H, Tashiro S
  • Journal Title: J Cell Sci Volume: 126 Pages: 5284-5292
  • DOI: 10.1242/jcs.133744
  • Peer Reviewed
• [Journal Article] Involvement of homologous recombination in the synergism between cisplatin and poly(ADP-ribose) polymerase inhibition (2013)
  • Author(s): Sakogawa K, Aoki Y, Misumi K, Hamai Y, Emi M, Hihara J, Shi L, Kono K, Horikoshi Y, Sun J, Ikura T, Okada M, Tashiro S
  • Journal Title: Cancer Sci. 2013 Sep 5 Volume: 104 Issue: 12 Pages: 1593-1609
  • DOI: 10.1111/cas.12281
  • Peer Reviewed
• [Journal Article] Involvement of ribonucleotide reductase-M1 in 5-fluorouracil induced DNA damage in esophageal cancer cell lines. (2013)
  • Author(s): Aoki Y, Sakogawa K, Hihara J, Emi M, Hamai Y, Kono K, Shi L, Sun J, Kitao H, Ikura T, Niida H, Nakanishi M, Okada M, Tashiro S.
  • Journal Title: Int J Oncol. Volume: 42(6) Issue: 6 Pages: 1951-60
  • DOI: 10.3892/ijo.2013.1899
  • Peer Reviewed
• [Journal Article] A functional deficiency of TERA/VCP/p97 contributes to impaired DNA repair in multiple polyglutamine diseases. (2013)
  • Author(s): Fujita K, Nakamura Y, Oka T, Ito H, Tamura T, Tagawa K, Sasabe T, Katsuta A, Motoki K, Shiwaku H, Sone M, Yoshida C, Katsuno M, Eishi Y, Murata M, Taylor JP, Wanker EE, Kono K, Tashiro S, Sobue G, La Spada AR, Okazawa H.
  • Journal Title: Nat Commun. Volume: 4 Issue: 1 Pages: 1816-1816
  • DOI: 10.1038/ncomms2828
  • Peer Reviewed
• [Journal Article] A nonsynonymous polymorphism in semaphorin 3A as a risk factor for human unexplained cardiac arrest with documented ventricular fibrillation (2013)
  • Author(s): Nakano Y, Makita N, (32人中31番目) et al
  • Journal Title: PLOS Genet Volume: 9 Issue: 4 Pages: e1003364-e1003364
  • DOI: 10.1371/journal.pgen.1003364
  • NAID: 120006985866
  • Peer Reviewed / Open Access
• [Presentation] 放射線によるゲノム損傷と修復 (2014)
  • Author(s): 田代 聡
  • Organizer: 広島医学会総会シンポジウム
  • Place of Presentation: 広島医師会館、広島市
  • Year and Date: 2014-11-09
• [Presentation] 染色体ストレス応答の再構成と複合システムとしての理解 (2014)
  • Author(s): 田代 聡
  • Organizer: 第87回 日本生化学会大会シンポジウム
  • Place of Presentation: 京都国際会議場、京都市
  • Year and Date: 2014-10-15
• [Presentation] 医療放射線被ばくによるゲノム損傷評価法の確立 (2014)
  • Author(s): 田代 聡
  • Organizer: 日本放射線影響学会 第57回大会 シンポジウム
  • Place of Presentation: 鹿児島県民交流センター、鹿児島市
  • Year and Date: 2014-10-03
• [Presentation] 相同組換え修復における損傷クロマチン動態 (2014)
  • Author(s): 田代 聡
  • Organizer: 第52回 日本生物物理学会年会
  • Place of Presentation: 札幌コンベンションセンター、札幌市
  • Year and Date: 2014-09-26
  • Invited
• [Presentation] Nuclear topography of homologous recombinational repairp (2014)
  • Author(s): Tashiro S
  • Organizer: Cold Spring Harbor meeting
  • Place of Presentation: ニューヨーク（アメリカ）
  • Year and Date: 2014-08-23 – 2014-08-26
• [Presentation] 損傷クロマチンの細胞核内動態
  • Author(s): 田代 聡
  • Organizer: 日本放射線影響学会 第56回大会
  • Place of Presentation: 青森市
• [Presentation] SUMO修飾システムによるゲノム損傷部位へのRAD51集積制御
  • Author(s): 田代 聡
  • Organizer: 第72回 日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] 染色体ストレス応答ネットワーク研究の新機軸
  • Author(s): 田代 聡
  • Organizer: 第86回 日本生化学会大会
  • Place of Presentation: 横浜市