Analysis of a transcriptional network for potential pluripotency in primordial germ cells

• Project/Area Number: 25650065
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cell biology
• Research Institution: Kyoto University
• Principal Investigator: Kurimoto Kazuki 京都大学, 医学(系)研究科(研究院), 准教授 (20415152)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ChIP-seq / OCT4 / 始原生殖細胞 / 多能性幹細胞 / Oct3/4 / PGC

Outline of Final Research Achievements

Primordial germ cells (PGCs), precursors of sperm and oocyte, is an embryonic cell type, which potentially possess the pluripotency, a cellular ability to give rise to all of cell types in the body. The explicit pluripotency (e.g., pluripotent stem cells, mismigrate PGCs) is a harmful property, which give rise to teratoma, but the mechanism to make pluripotency to be a “potential” one is largely elusive. Recently, I developed a method to faithfully identify genomic binding sites of transcription factors in a small number of cells (~10,000 cells). In this study, I investigated application of this method to pluripotency transcription factors expressed in PGCs, and identified the OCT4 binding pattern in PGC-like cells distinct form that of ES cells, suggesting a transcription network characteristic of potential pluripotency.

Research Products

• [Journal Article] Persistent requirement and alteration of the key targets of PRDM1 during primordial germ cell development in mice. (2015)
  • Author(s): Yamashiro C, Hirota T, Kurimoto K, Nakamura T, Yabuta Y, Nagaoka SI, Ohta H, Yamamoto T, Saitou M.
  • Journal Title: Biology of Reproduction Volume: 94 Issue: 1 Pages: 7-15
  • DOI: 10.1095/biolreprod.115.133256
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells (2015)
  • Author(s): Sasaki, K., Yokobayash, S., Nakamura, T., Okamoto, I., Yabuta, Y., Kurimoto, K., Ohta, H., Moritoki, Y., Iwatani, C., Tsuchiya, H., Nakamura, S., Sekiguchi, K., Sakuma, T., Yamamoto, T., Mori, T., Woltjen, K., Nakagawa, M., Yamamoto, T., Takahashi, K., Yamanaka, S., Saitou, M.
  • Journal Title: Cell Stem Cell Volume: 17 Issue: 2 Pages: 178-94
  • DOI: 10.1016/j.stem.2015.06.014
  • Peer Reviewed
• [Journal Article] Mechanism and Reconstitution In Vitro of Germ Cell Development in Mammals (2015)
  • Author(s): Kurimoto, K., Saitou, M.
  • Journal Title: Cold Spring Harb Symp Quant Biol Volume: LXXX Pages: 1-7
  • DOI: 10.1101/sqb.2015.80.027425
• [Journal Article] 始原生殖細胞の試験管内再構成系とその応用：エピゲノムイリプログラミング機構の解明に向けて (2015)
  • Author(s): 栗本一基, 斎藤通紀
  • Journal Title: 日本生殖内分泌学会雑誌 Volume: 20 Pages: 7-13
• [Journal Article] Quantitative Dynamics of Chromatin Remodeling during Germ Cell Specification from Mouse Embryonic Stem Cells (2015)
  • Author(s): Kurimoto, K., Yabuta, Y., Hayashi, K., Ohta, H., Kiyonari, H., Mitani, T., Moritoki, Y., Kohri, K., Kimura, H., Yamamoto, T., Katou, Y., Shirahige, K., Saitou, M.
  • Journal Title: Cell Stem Cell Volume: 16 Issue: 5 Pages: 517-532
  • DOI: 10.1016/j.stem.2015.03.002
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] SC3-seq: a method for highly parallel and quantitative measurement of single-cell gene expression (2015)
  • Author(s): Nakamura, T., Yabuta, Y., Okamoto, I., Aramaki, S., Yokobayashi, S., Kurimoto, K., Sekiguchi, K., Nakagawa, M., Yamamoto, T., Saitou, M.
  • Journal Title: Nucleic Acids Res Volume: - Issue: 9 Pages: e60-e60
  • DOI: 10.1093/nar/gkv134
  • NAID: 120005825922
  • Peer Reviewed / Open Access
• [Journal Article] Paternal nucleosomes: are they retained in developmental promoters or gene deserts? (2014)
  • Author(s): Saitou, M., Kurimoto, K.
  • Journal Title: Dev Cell Volume: 30 Issue: 1 Pages: 6-8
  • DOI: 10.1016/j.devcel.2014.06.025
• [Journal Article] CCR1-mediated accumulation of myeloid cells in the liver microenvironment promoting mouse colon cancer metastasis (2014)
  • Author(s): Hirai H, Fujishita T, Kurimoto K, Miyachi H, Kitano S, Inamoto S, Itatani Y, Saitou M, Maekawa T, Taketo MM
  • Journal Title: Clin Exp Metastasis Volume: 31 Issue: 8 Pages: 977-989
  • DOI: 10.1007/s10585-014-9684-z
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cell-to-cell expression variability followed by signal reinforcement progressively segregates early mouse lineages (2014)
  • Author(s): Ohnishi, Y., Huber, W., Tsumura, A., Kang, M., Xenopoulos, P., Kurimoto, K., Oles, A. K., Arauzo-Bravo, M. J., Saitou, M., Hadjantonakis, A. K., Hiiragi, T.
  • Journal Title: Nat Cell Biol Volume: 16 Issue: 1 Pages: 27-37
  • DOI: 10.1038/ncb2881
  • Peer Reviewed
• [Journal Article] A mesodermal factor, T, specifies mouse germ cell fate by directly activating germline determinants (2013)
  • Author(s): Aramaki, S., Hayashi, K., Kurimoto, K., Ohta, H., Yabuta, Y., Iwanari, H., Mochizuki, Y., Hamakubo, T., Kato, Y., Shirahige, K., and Saitou, M
  • Journal Title: Developmental Cell Volume: 27 Issue: 5 Pages: 516-529
  • DOI: 10.1016/j.devcel.2013.11.001
  • Peer Reviewed / Open Access
• [Journal Article] Induction of mouse germ-cell fate by transcription factors in vitro. (2013)
  • Author(s): Nakaki F, Hayashi K, Ohta H, Kurimoto K, Yabuta Y, Saitou M.
  • Journal Title: Nature Volume: 501 Issue: 7466 Pages: 222-226
  • DOI: 10.1038/nature12417
  • Peer Reviewed
• [Presentation] 単一細胞遺伝子発現解析によるマウス始原生殖細胞の発生機構の解明 (2015)
  • Author(s): 栗本一基, 薮田幸宏, 斎藤通紀
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Presentation] Quantitative Dynamics of Chromatin-State Reprogramming of Mouse Germ Cell Specification Pathway In Vitro (2015)
  • Author(s): Kurimoto, K
  • Organizer: Gordon Research Conference, Germinal Stem Cell Biology
  • Place of Presentation: The Chinese University of Hong Kong
  • Year and Date: 2015-06-03
  • Int'l Joint Research / Invited