| Project/Area Number |
25670612
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurosurgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Naoki 北海道大学, 大学院医学研究科, 講師 (40421961)
KAZUMATA Ken 北海道大学, 大学病院, 講師 (60634144)
ABUMIYA Takeo 北海道大学, 大学院医学研究科, 特任助教 (80270726)
SHICHINOHE Hideo 北海道大学, 大学病院, 助教 (80374479)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 脳微小血管 / 細胞老化 / SIRT1 |
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| Outline of Final Research Achievements |
The present study was performed with cultured senescent bovine brain endothelial cells (BMEC) with high passage numbers. P15-20 senescent BMEC contained giant cells with increase of β-galactosidase activity and reactive oxygen species. In comparison between P5 BMEC and P 15 BMEC subjected to oxygen-glucose deprivation and reoxygenation, P15 BMEC showed significantly more cell death and apoptosis. P5 BMEC showed significantly more induction of SIRT1 expression. This result suggests that SIRT1 seems to have cellular protective effects and lose its capacity of induction in senescent cells.
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