Correlation between cell cycle and chemotherapy resistance and lung metastases - Analysis using Fucci transfected sarcoma cells

• Project/Area Number: 25861301
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Nagoya City University (2014-2015); Kanazawa University (2013)
• Principal Investigator: KIMRA HIROAKI 名古屋市立大学, 医学(系)研究科(研究院), 助教 (50608693)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 細胞周期 / イメージング / 骨肉腫 / 化学療法 / 紫外線 / 蛍光蛋白 / リアルタイムイメージング / FUCCI / Fucci

Outline of Final Research Achievements

we utilized the fluorescence ubiquitination-based cell cycle indicator (FUCCI) imaging system to investigate the correlation between cell-cycle behavior and apoptosis after treatment of osteosarcoma cells with chemotherapeutic drugs.
Osteosarcoma cell line 143B expressing FUCCI were treated with doxorubicin or cisplatinum, and we revealed that apoptosis behavior, cell cycle arrest, and chemotherapy sensitivity depending on cell cycle differs in each chemotherapy drugs.

Research Products

• [Journal Article] Heterogeneous cell-cycle behavior in response to UVB irradiation by a population of single cancer cells visualized by time-lapse FUCCI imaging. (2015)
  • Author(s): Miwa S, Yano S, Kimura H, Yamamoto M, Toneri M, Murakami T, Hayashi K, Yamamoto N, Fujiwara T, Tsuchiya H, Hoffman RM.
  • Journal Title: Cell Cycle Volume: 14 Issue: 12 Pages: 1932-1937
  • DOI: 10.1080/15384101.2015.1033598
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cell-cycle fate-monitoring distinguishes individual chemosensitive and chemoresistant cancer cells in drug-treated heterogeneous populations demonstrated by real-time FUCCI imaging. (2015)
  • Author(s): Miwa S, Yano S, Kimura H, Yamamoto M, Toneri M, Matsumoto Y, Uehara F, Hiroshima Y, Murakami T, Hayashi K, Yamamoto N, Bouvet M, Fujiwara T, Tsuchiya H, Hoffman RM.
  • Journal Title: Cell cycle Volume: 14 Issue: 4 Pages: 621-629
  • DOI: 10.4161/15384101.2014.991604
  • Peer Reviewed / Open Access / Acknowledgement Compliant