| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Human dental pulp stem cells (hDPSCs) isolated by plastic adherence, frequently contain contaminating hematopoietic cells and mature cells, which has limited our ability to investigate their basic biology and regenerative properties. In the present study, we prospectively isolated a pure population of hDPSCs from human dental pulp by flow cytometry. Prospectively isolated, dental pulp-derived LNGFR Low+/THY-1 High+ cells represent a highly enriched population of clonogenic cells; notably, the isolated cells exhibited high proliferation and multi-lineage differentiation potential in vitro. The cells also promoted new bone formation to heal critical-size calvarial defects in vivo. These findings suggest that LNGFR Low+/THY-1 High+ dental pulp-derived cells provide an excellent source of material for bone regenerative strategies.
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