Analysis of highly expressed miR-629 in clear cell renal cell carcinoma

• Project/Area Number: 25893113
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Osaka University
• Principal Investigator: JINGUSHI Kentaro 大阪大学, 薬学研究科(研究院), 研究員 (80707571)
• Research Collaborator: TSUJIKAWA Kazutake ; NONOMURA Norio ; UEMURA Motohide ; FUJITA Kazutoshi
• Project Period (FY): 2013-08-30 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: miR-629 / TGF-bシグナル伝達経路 / ccRCC / EMT / TGF-β/Smadシグナル伝達経路

Outline of Final Research Achievements

This is the first study showing significant upregulation of miR-629 in ccRCC specimens by quantitative real-time PCR analysis. We demonstrate that miR-629 downregulates TRIM33 expression, leading to the association of Smad2/3 and Smad4 and then to the promotion of the TGFb/Smad signaling pathway. Moreover, we clarify that TRIM33 is significantly downregulated in ccRCC tissues compared with that in the adjacent noncancerous renal tissues, which seems to correlate with pathologic stages and grades. Our findings show that miR-629 is a potent regulator of the TGFb/Smad signaling pathway and accelerates ccRCC cell motility and invasion.

Research Products

• [Journal Article] miR-629 Targets TRIM33 to Promote TGFb/Smad Signaling and Metastatic Phenotypes in ccRCC (2015)
  • Author(s): Kentaro Jingushi, Yuko Ueda, Kaori Kitae, Hiroaki Hase, Hiroshi Egawa, Ikumi Ohshio, Ryoji Kawakami, Yuri Kashiwagi, Yohei Tsukada, Takumi Kobayashi, Wataru Nakata, Kazutoshi Fujita, Motohide Uemura, Norio Nonomura, and Kazutake Tsujikawa
  • Journal Title: Molecular Cancer Research Volume: 13 Issue: 3 Pages: 565-574
  • DOI: 10.1158/1541-7786.mcr-14-0300
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 淡明細胞型腎細胞癌においてmiR-629はTRIM33を介してTGF-b/Smadシグナルを促進する (2014)
  • Author(s): 神宮司 健太郎、江川 博、中田 渡、藤田和利、植村 元秀、野々村 祝夫、辻川 和丈
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜 (横浜)
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] MiR-629 targets TRIM33 to promote TGF-b/Smad signaling in clear cell renal cell carcinoma (2014)
  • Author(s): Kentaro Jingushi, Wataru Nakata, Yuko Ueda, Kaori Kitae, Kazutoshi Fujita, Motohide Uemura, Norio Nonomura, Kazutake Tsujikawa
  • Organizer: AACR annual meeting 2014
  • Place of Presentation: San Diego Convention Center (San Diego)
  • Year and Date: 2014-04-05 – 2014-04-09
• [Presentation] MiR-629 targets TRIM33 to promote TGF-β/Smad signaling in clear cell renal cell carcinoma (2014)
  • Author(s): Kentaro Jingushi, Wataru Nakata, Yuko Ueda, Kaori Kitae, Kazutoshi Fujita, Motohide Uemura, Norio Nonomura, Kazutake Tsujikawa
  • Organizer: American Association for Cancer Research Annual Meeting 2014
  • Place of Presentation: San Diego
• [Remarks] 大阪大学大学院薬学研究科細胞生理学分野
  • URL: http://www.phs.osaka-u.ac.jp/homepage/b008/