| Project/Area Number |
26460474
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
Mitaka Toshihiro 札幌医科大学, 医学部, 教授 (50231618)
Tanimizu Naoki 札幌医科大学, 医学部, 准教授 (00333386)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 細胞・組織 / 再生医学 / 肝幹・前駆細胞 / 細胞移植 / 生体組織工学 / 骨髄間葉系細胞 / IL17B/IL25 / Extracellular vesicles / 肝再生 |
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| Outline of Final Research Achievements |
In retrorsine (Ret)/70% partial hepatectomy (PH) model rat livers small hepatocyte-like progenitor cells (SHPCs) transiently appear to form clusters. However, the molecular mechanisms regulating their proliferation remain unknown. We found that transplantation of Thy1+ cells isolated from galactosamine-treated rat livers transiently increased the liver mass by expanding SHPCs in Ret/PH model livers. In this study, we examined the mechanism of the enhanced liver regeneration. Extracellular vesicles secreted from Thy1+ cells induced interleukin 17 b (IL17b) and IL25 expression in sinusoidal endothelial cells and Kupffer cells, respectively, whereas they induced IL17 receptor b (IL17rb) in SHPCs. In conclusion, Thy1+ cells transplantation enhanced the growth of intrinsic hepatic progenitor cells via IL17RB signals.
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