| Project/Area Number |
26460647
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Shinshu University |
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Principal Investigator |
HIDAKA Hiroya 信州大学, 学術研究院保健学系, 准教授 (10362138)
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| Co-Investigator(Kenkyū-buntansha) |
本郷 実 信州大学, 学術研究院保健学系, 教授 (40209317)
本田 孝行 信州大学, 学術研究院医学系, 教授 (80238815)
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| Research Collaborator |
YAMAMOTRO Hiroshi
NAKAMURA Tomohiko
HIDAKA Eiko
SUGANO Mitsutoshi
KAWASAKI Kenji
ISHIMINE Nau
KIDOKUCHI Shuhei
NISHIJIMA Fumie
HORI Atsushi
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 質量分析 / ヒト血液 / 脂質分子種 / 脂質分子 / リン脂質 / MALDI-TOF質量分析 / グリセロリン脂質 / スフィンゴ脂質 / 脂質代謝酵素 / クロマトグラフィ / ヒト血清 / ヒト気管支肺胞洗浄液 |
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| Outline of Final Research Achievements |
Phospholipids and their metabolites function as regulators of lipid metabolism and are associated with various diseases. Lipid molecular species in human biomedical materials, such as blood and bronchoalveolar lavage fluid, were analyzed using the methods of gel chromatography and Matrix assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS), Liquid chromatography MS, and Gas chromatography MS.Furthermore, we generated lipid species profiles of healthy subjects and then visualized these molecular species. We also showed that these methods for lipid molecular species analysis can be used in clinical laboratory tests. We are currently accumulating clinical data to identify specific lipid molecular species associated with pathological conditions.
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