Cytokines secreted from the liver affect bone mass.

• Project/Area Number: 26461377
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Endocrinology
• Research Institution: Kanazawa University
• Principal Investigator: Ishii Kiyoaki 金沢大学, 医学系, 特任准教授 (80419150)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 骨粗鬆症 / 糖尿病 / 老化 / 内分泌 / 肥満 / へパトカイン

Outline of Final Research Achievements

We measured the bone morphology in Lect 2 knockout mice and found that the bone mass decreased as compared with the wild type mouse. Examination of bone metabolism markers in serum showed an increase in osteoclast activation marker TRAP 5b in Lect 2 KO mice. Even at the cellular level, osteoclasts were found to inhibit the differentiation of macrophages into mature osteoclasts by addition of Lect 2. In osteoblasts, there was no significant effect on the differentiation stage. However, in the postmenopausal osteoporosis model from which the ovaries were removed, there was no difference in the extent of bone loss in wild type and Lect 2 KO mice.

Research Products

• [Journal Article] Rapid response of the steatosis-sensing hepatokine LECT2 during diet-induced weight cycling in mice. (2016)
  • Author(s): K Chikamoto, H Misu, H Takayama, A Kikuchi, KA Ishii, F Lan, N Takata, N Tajima-Shirasaki, Y Takeshita, H Tsugane, S Kaneko, S Matsugo, T Takamura.
  • Journal Title: Biochem Biophys Res Commun Volume: 478 Issue: 3 Pages: 1310-1316
  • DOI: 10.1016/j.bbrc.2016.08.117
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Crucial Role of Elovl6 in Chondrocyte Growth and Differentiation during Growth Plate Development in Mice. (2016)
  • Author(s): Kikuchi M, Shimada M, Matsuzaka T, Ishii K, Nakagawa Y, Takayanagi M, Yamada N, Shimano H.
  • Journal Title: PLoS One. Volume: 11(7) Issue: 7 Pages: e0159375-e0159375
  • DOI: 10.1371/journal.pone.0159375
  • NAID: 120007129433
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Loss of SDHB Elevates Catecholamine Synthesis and Secretion Depending on ROS Production and HIF Stabilization. (2016)
  • Author(s): Saito Y, Ishii KA, Aita Y, Ikeda T, Kawakami Y, Shimano H, Hara H, Takekoshi K.
  • Journal Title: Neurochem Res. Volume: 41(4) Issue: 4 Pages: 696-706
  • DOI: 10.1007/s11064-015-1738-3
  • Peer Reviewed
• [Journal Article] 非アルコール性脂肪性肝疾患 (NAFLD) / 非アルコール性脂質性炎（NASH）と栄養 (2016)
  • Author(s): 石井 清朗, 篁 俊成
  • Journal Title: CLINICAL CALCIUM Volume: 26-3 Pages: 312-316
• [Journal Article] Non-alcoholic fatty liver disease (NAFLD) /non-alcoholic steatohepatitis (NASH) and nutrition (2016)
  • Author(s): Ishii KA, Takamura T
  • Journal Title: Clinical calcium Volume: 26(3) Pages: 363-7
• [Journal Article] 肝臓由来のインスリン感受性制御 (2015)
  • Author(s): 石井清朗、篁俊成
  • Journal Title: Diabetes Frontier Volume: Vol.23 No.3 Pages: 312-6