Pathogenesis of insufficient adrenocortical response in premature sheep fetus
| Project/Area Number |
26461627
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
齋藤 昌利 東北大学, 大学病院, 講師 (00451584)
埴田 卓志 東北大学, 大学病院, 助手 (30400360)
松田 直 東北大学, 大学病院, 准教授 (50361100)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 胎児 / 早産児 / 子宮内炎症 / 副腎皮質ホルモン / CRH負荷試験 / ヒツジ / 胎仔 / ACTH / cortisol / cortisone / 人工胎盤 / 虚血 / 炎症 / コーチゾル分泌能 / ポンプレス人工胎盤システム / ヒツジ胎仔 |
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| Outline of Final Research Achievements |
We made a plan to clarify the effect of intrauterine inflammation on the cortisol secretion and its circulatory response in premature lamb because the fetal endocrine potential to adapt the postnatal environment might have been already deteriorated by the inflammatory stress before birth. In this study, we separated sheep fetuses into two groups: control group (n=6) and inflammation group (n=6), and we could store the plasma samples for 31 times of the corticotrophin releasing hormone (CRH) test. In consequence, we could measured the concentrations of ACTH, cortisol, cortisone, Aldosterone and Dehydroepiandrosterone sulfate (DHEA-S) by the method of steroid profiling analysis using the LC-MS/MS. In the inflammation group than the control, production of cortisol was promoted during the CRH test. These findings suggest the intrauterine inflammation stress might induce the development of transitional zones where 3β-hydroxysteroid dehydrogenase is expressed.
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Report
(4 results)
Research Products
(1 results)
