Efficacy of high density hydrogen water for the treatment of chemotherapy induced skin disease
| Project/Area Number |
26461956
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Kyoto Prefectural University of Medicine |
|---|
Principal Investigator |
Taguchi Tetsuya 京都府立医科大学, 医学(系)研究科(研究院), 教授 (80243260)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
阪口 晃一 京都府立医科大学, 医学(系)研究科(研究院), 講師 (00405284)
|
|---|
| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | 癌 / 化学療法 / 分子標的薬 / 抗癌剤 / 皮膚障害 / ヒト角化皮膚正常細胞株 / アポトーシス / ROS / 手足症候群 / afatinib / lapatinib / 5-FU / 水素水 / 有害事象 / EGFR / TKI / Lapatinib / Afatinib / Ha-cat細胞 / 分子標的剤 / 抗EGFR / ヒト角化表皮細胞 / 活性酸素 / 表皮角化上皮細胞 |
|---|
| Outline of Final Research Achievements |
First of all, inhibitory effect of proliferation by afatinib, lapatinib and 5-FU to Ha-Cat (human keratinocyte) cell line was confirmed by WST-8 assay. And according to the FACS analysis, this proliferation suppression is likely to due to apoptosis instead of the cell cycle arrest. In addition, caspase independent mechanism was revealed by zVAD added pan-caspase inhibitory assay. Finally, according to the pan-Ros indicator and pan-ROS scavenger assay, this cell death was thought to be due to ROS. But we could not identify the specific type of ROS using specific ROS indicator assay.
|
Report
(5 results)
Research Products
(2 results)
