| Project/Area Number |
26670341
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hygiene and public health
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| Research Institution | National Institute for Environmental Studies |
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Principal Investigator |
Hirano Seishiro 国立研究開発法人国立環境研究所, 環境リスク研究センター, 室長 (20150162)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 粒子状物質 / マクロファージ / エンドサイトーシス / 貪食レセプター / インフラマゾーム / 貪食 / ピノサイトーシス / エンドサートーシス |
|---|
| Outline of Final Research Achievements |
The aim of this study was to reveal how fibrous particulate substances induce inflammasome and inflammation including IL-1beta production in macrophages. This author first investigated the internalization of fibrous particles via a phagocytic receptor and found that the fibers were taken up by the cells via micropinocytosis as well as phagocytosis. Co-exposure to lipopolysaccharide and fibrous titanium dioxide nano-fibers significantly increased release of IL-1beta in macrophages. When, lysosomal function was artificially deteriorated by chemicals such as ammonium carbonate, bafilomycin, and chloroquine, the amount of LC3-II and the LC3-II/LC3-I ratio were increased and this increase was strongly associated with that of p62/SQSTM1, suggesting that autophagy is involved in the inflammasome formation.
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