Study of ncRNA for the establishment of a new biomarker in radiation therapy

• Project/Area Number: 26830096
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: The University of Tokyo
• Principal Investigator: Miyagawa Ryu 東京大学, 医学部附属病院, 助教 (20721939)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 非コードRNA / 放射線治療 / がん / バイオマーカー / 放射線 / ncRNA / lncRNA

Outline of Final Research Achievements

The purpose of this study is to elucidate the new mechanisms of radiation-responsive non-coding RNA (ncRNA) for the establishment of a new biomarker in radiation therapy. I used X-ray-irradiated cancer-derived cultured cells. First, I identified the expressoion change of some ncRNAs by qPCR. Concurrently, I also identified the localization change of some ncRNAs and some proteins by FISH and IF methods. But I was not able to identify the interaction of those ncRNAs with proteins.

Research Products

• [Journal Article] Formation of Klotho Granules in Oxidative Stress-induced Human Cancer Cells (2014)
  • Author(s): Ryu Miyagawa, Rie Mizuno, Kenichi Ijiri, Satoshi Fujii
  • Journal Title: Bioimages. Volume: 12 Pages: 17-21
  • NAID: 130005086188
  • Peer Reviewed / Open Access
• [Journal Article] 長塩基長非コードRNAを切り口にした放射線生物研究 (2014)
  • Author(s): 宮川隆、小林伶子、片野厚人、中川恵一
  • Journal Title: Radiation Biology Research Communications Volume: 49 Pages: 303-317
  • NAID: 40020222424
  • Peer Reviewed
• [Presentation] A Study on the Specific tRNA Granule Aiming for Erection of New Biomarker in Radiation Therapy. (2015)
  • Author(s): Ryu Miyagawa, Atsuto Katano, Reiko Kobayashi, Hideomi Yamashita, Teppei Morikawa, Masashi Fukayama, Kuni Ohtomo, Keiichi Nakagawa
  • Organizer: 15th International Congress of Radiation Research
  • Place of Presentation: 京都
  • Year and Date: 2015-05-27
  • Int'l Joint Research
• [Presentation] long non-coding RNA XIST の高発現は、子宮頸癌CRT後の予後良好因子である (2014)
  • Author(s): 小林伶子、宮川隆、山下英臣、森川鉄平、大熊加恵、深山正久、大友邦、中川恵一
  • Organizer: 日本放射線腫瘍学会第27回学術大会
  • Place of Presentation: 横浜
  • Year and Date: 2014-12-11 – 2014-12-13
• [Remarks] 文部科学省科学研究費新学術領域研究 がん研究分野の特性等を踏まえた支援活動
  • URL: http://ganshien.umin.jp/research/spotlight/miyagawa/index.html