| Project/Area Number |
26850004
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 植物分子育種 / オオムギ / 形質転換 / ゲノム編集 / TALEN |
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| Outline of Final Research Achievements |
Transcription activator-like effector nuclease (TALEN)-vectors targeting for the genes encoding chalcone synthase (CHS) and dihydroflavonol 4-reductase (DFR) which were both involved in anthocyanin biosynthesis were constructed and applied to barley transformation. Although transgenic barley plants were obtained, any mutation was detected in target genes of these plants.
On the other hand, 4 promoters regulating callus-specific gene expression were isolated in barley. For expression analysis, these promoters were cloned into expression vector of the gene encoding enhanced green fluorescent protein (EGFP). The transient expression of EGFP in callus was observed by all the promoters. In addition, 2 of 4 promoters showed stable expression of EGFP in transgenic callus in barley.
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