Cellular dynamics of the anterior second heart field during heart development

• Project/Area Number: 26860127
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology)
• Research Institution: National Cardiovascular Center Research Institute (2015); Tohoku University (2014)
• Principal Investigator: Watanabe Yusuke 国立研究開発法人国立循環器病研究センター, 研究所, 室長 (20562333)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 心臓 / 発生 / 細胞動態 / 心臓発生

Outline of Final Research Achievements

In this study, I aimed to understand cellular dynamics of anterior second heart field (aSHF) cells which contribute to the outflow tract and right ventricle of the heart.
I observed mouse embryos which expresses fluorescent proteins in cardiac mesoderm. As a result, aSHF region seemed to contribute to the outflow tract with dynamic remodeling of tissues. This suggests that the outflow tract is formed by incorporation of aSHF region to the heart with tissue remodeling of embryo.

Research Products

• [Journal Article] Pharyngeal arch artery defects and lethal malformations of the aortic arch and its branches in mice deficient for the Hrt1/Hey1 transcription factor. (2016)
  • Author(s): Fujita M, Sakabe M, Ioka T, Watanabe Y, Kinugasa-Katayama Y, Tsuchihashi T, Utset MF, Yamagishi H, Nakagawa O.
  • Journal Title: Mechanisms of Development Volume: 139 Pages: 65-73
  • DOI: 10.1016/j.mod.2015.11.002
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Reconstruction and in vivo analysis of the extinct tbx5 gene from ancient wingless moa (Aves: Dinornithiformes) (2014)
  • Author(s): Huynen L. et al.
  • Journal Title: BMC Evolutionary Biology Volume: 14 Issue: 1 Pages: 75-82
  • DOI: 10.1186/1471-2148-14-75
  • Peer Reviewed / Open Access
• [Journal Article] Prdm1 functions in the mesoderm of the second heart field, where it interacts genetically with Tbx1, during outflow tract morphogenesis in the mouse embryo. (2014)
  • Author(s): Vincent SD, Mayeuf-Louchart A, Watanabe Y, Brzezinski JA 4th, Miyagawa-Tomita S, Kelly RG, Buckingham M.
  • Journal Title: Hum Mol Genet. Volume: 23 Issue: 19 Pages: 5087-5101
  • DOI: 10.1093/hmg/ddu232
  • Peer Reviewed
• [Journal Article] New BRAF knockin mice provide a pathogenetic mechanism of developmental defects and a therapeutic approach in cardio-facio-cutaneous syndrome. (2014)
  • Author(s): Inoue S. I. et al.
  • Journal Title: Hum. Mol. Genet. Volume: 23 Issue: 24 Pages: 6553-6566
  • DOI: 10.1093/hmg/ddu376
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Transcriptional regulation by competitive DNA binding of Islet1 and Nkx2-5 (2015)
  • Author(s): Yusuke Watanabe
  • Organizer: 心血管代謝週間 ISHR Japan Section
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2015-12-10
  • Int'l Joint Research / Invited
• [Presentation] 胎生期内皮遺伝子Tmem100の心血管形態形成における意義 (2015)
  • Author(s): 片山由美、渡邉裕介、水田賢、坂部正英、井岡朋子、荒木睦、石井修平、染川智、斎藤能彦、中川修
  • Organizer: 心血管代謝週間 日本血管生物医学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2015-12-10
• [Presentation] 血管内皮細胞におけるBMP9/BMP10-ALK1シグナル下流遺伝子群の解析 (2015)
  • Author(s): 荒木睦、渡邉裕介、坂部正英、久光隆、中尾周、藤田匡秀、片山由美、井岡朋子、中川修
  • Organizer: 心血管代謝週間 日本血管生物医学会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2015-12-10
• [Book] 循環器病研究の進歩 (2015)
  • Author(s): 渡邉裕介、久光隆、片山由美、荒木睦、石井修平、藤田匡秀、坂部正英、中川修
  • Total Pages: 8
  • Publisher: 株式会社 協和企画
• [Remarks] 国立循環器病研究センター、研究所、分子生理部ホームページ
  • URL: http://www.ncvc.go.jp/res/divisions/molecular_physiology/